##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccI AccIII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AviII AvrII BalI
BanI BbeI BbrPI BclI BglI
BlnI BsaAI BsaI BsaWI BseAI
BsiWI BspEI BspHI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
CpoI DraIII Eco47III EcoRV EcoT22I
FbaI FseI FspI HaeII KasI
KpnI MluI MluNI MroI MscI
NarI NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI PvuI RcaI RsrII
SacI SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AflII 2406 CTTAAG C^TTAAG
AhdI 3464 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2256 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3464 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4917 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 5044 GCTGAGG CCTCAGC(-5/-2)
BfrI 2406 CTTAAG C^TTAAG
BsgI 1021 CTGCAC GTGCAG(16/14)
BsiEI 286 CGGCCG CG[GA][CT]^CG
BsmI 1985 GCATTC GAATGC(1/-1)
BspDI 2194 ATCGAT AT^CGAT
BsrBI 219 GAGCGG CCGCTC(-3/-3)
CfoI 265 GCGC GCG^C
ClaI 2194 ATCGAT AT^CGAT
EaeI 286 CGGCCG [CT]^GGCC[GA]
EagI 286 CGGCCG C^GGCCG
Eam1105I 3464 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 286 CGGCCG C^GGCCG
Eco52I 286 CGGCCG C^GGCCG
EcoRI 4108 GAATTC G^AATTC
HhaI 265 GCGC GCG^C
HinP1I 265 GCGC G^CGC
NcoI 3146 CCATGG C^CATGG
NdeI 1857 CATATG CA^TATG
PaeR7I 2170 CTCGAG C^TCGAG
PflFI 4917 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 318 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2370 GAAGAGC GCTCTTC(1/4)
SexAI 794 ACCTGGT A^CC[AT]GGT
SmaI 310 CCCGGG CCC^GGG
Tth111I 4917 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4987 TCTAGA T^CTAGA
XhoI 2170 CTCGAG C^TCGAG
XmaCI 310 CCCGGG C^CCGGG
XmaI 310 CCCGGG C^CCGGG
XmnI 2256 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 281 GACGCC G[GA]^CG[CT]C
318 GACGCC
AflIII 1037 ACATGT A^C[GA][CT]GT
5810 ACATGT
AocI 3206 CCTTAGG CC^T[ACGT]AGG
4035 CCTAAGG
BamHI 1154 GGATCC G^GATCC
1928 GGATCC
BsaHI 281 GACGCC G[GA]^CG[CT]C
318 GACGCC
BsmBI 431 CGTCTC CGTCTC(1/5)
83 GAGACG
BspLU11I 1037 ACATGT A^CATGT
5810 ACATGT
BsrDI 267 GCAATG GCAATG(2/0)
3688 GCAATG
Bsu36I 3206 CCTTAGG CC^T[ACGT]AGG
4035 CCTAAGG
Eco81I 3206 CCTTAGG CC^T[ACGT]AGG
4035 CCTAAGG
EcoNI 4031 CCTCCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5390 CCTTCAAAAGG
HgaI 281 GACGC GACGC(5/10)
318 GACGC
Hin1I 281 GACGCC G[GA]^CG[CT]C
318 GACGCC
HpaI 2234 GTTAAC GTT^AAC
5749 GTTAAC
KspI 138 CCGCGG CCGC^GG
260 CCGCGG
MfeI 835 CAATTG C^AATTG
2779 CAATTG
MunI 835 CAATTG C^AATTG
2779 CAATTG
NaeI 284 GCCGGC GCC^GGC
2469 GCCGGC
NgoMIV 284 GCCGGC G^CCGGC
2469 GCCGGC
PvuII 234 CAGCTG CAG^CTG
4576 CAGCTG
SacII 138 CCGCGG CCGC^GG
260 CCGCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AclI AcsI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BanII BbsI
BbvI BcgI BciVI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrFI BsrGI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstYI Cac8I CelII
Cfr10I Cfr13I DdeI DpnII DraI
DraII DrdI DsaI EarI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI Psp1406I
PstI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII