##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AclI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI BbeI BcgI BciVI BlpI
Bpu1102I BsgI BsiWI BsmBI BssHII
BstEII BstPI CelII CpoI DraII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoO65I FseI HgaI HpaI
KasI KpnI KspI MluI NaeI
NarI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PinAI PpuMI
PshAI Psp1406I PvuI RsrII SacII
SbfI ScaI SexAI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 1427 GACGTC GACGT^C
AccIII 3190 TCCGGA T^CCGGA
AcyI 1427 GACGTC G[GA]^CG[CT]C
AflII 2151 CTTAAG C^TTAAG
AflIII 5316 ACATGT A^C[GA][CT]GT
AspI 4266 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4366 CCCGAG C^[CT]CG[GA]G
AviII 2554 TGCGCA TGC^GCA
AvrII 2328 CCTAGG C^CTAGG
BalI 4481 TGGCCA TGG^CCA
BanI 4426 GGTGCC G^G[CT][GA]CC
BbrPI 1486 CACGTG CAC^GTG
BfrI 2151 CTTAAG C^TTAAG
BglI 4393 GCCATCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2328 CCTAGG C^CTAGG
BsaHI 1427 GACGTC G[GA]^CG[CT]C
BseAI 3190 TCCGGA T^CCGGA
BsiEI 4352 CGACCG CG[GA][CT]^CG
BsoBI 4366 CCCGAG C^[CT]CG[GA]G
BspDI 3148 ATCGAT AT^CGAT
BspEI 3190 TCCGGA T^CCGGA
BspLU11I 5316 ACATGT A^CATGT
BsrBI 4071 GAGCGG CCGCTC(-3/-3)
BsrFI 1126 ACCGGC [GA]^CCGG[CT]
BssSI 3933 CACGAG CACGAG(-5/-1)
BstAPI 2935 GCATGCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 320 CCATGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1126 ACCGGC [GA]^CCGG[CT]
ClaI 3148 ATCGAT AT^CGAT
DrdI 1421 GACATCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 4481 TGGCCA [CT]^GGCC[GA]
FauI 2104 GCGGG CCCGC(4/6)
FspI 2554 TGCGCA TGC^GCA
Hin1I 1427 GACGTC G[GA]^CG[CT]C
MfeI 2558 CAATTG C^AATTG
MluNI 4481 TGGCCA TGG^CCA
MroI 3190 TCCGGA T^CCGGA
MscI 4481 TGGCCA TGG^CCA
MunI 2558 CAATTG C^AATTG
PflFI 4266 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1486 CACGTG CAC^GTG
PmeI 4906 GTTTAAAC GTTT^AAAC
PmlI 1486 CACGTG CAC^GTG
SalI 4350 GTCGAC G^TCGAC
SfiI 4392 GGCCATCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2935 GCATGC GCATG^C
StuI 893 AGGCCT AGG^CCT
Tth111I 4266 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 4439 CCACCCACTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AocI 4111 CCTCAGG CC^T[ACGT]AGG
4614 CCTGAGG
BamHI 1959 GGATCC G^GATCC
3711 GGATCC
BclI 1482 TGATCA T^GATCA
2765 TGATCA
BcnI 4355 CCGGG CC^[GC]GG
4398 CCGGG
BglII 1003 AGATCT A^GATCT
1638 AGATCT
BsaAI 1486 CACGTG [CT]AC^GT[GA]
3379 TACGTG
BsaI 41 GAGACC GGTCTC(1/5)
1463 GGTCTC
BsmI 569 GAATGC GAATGC(1/-1)
771 GCATTC
Bsp1407I 1184 TGTACA T^GTACA
3606 TGTACA
BspHI 1527 TCATGA T^CATGA
3371 TCATGA
BsrGI 1184 TGTACA T^GTACA
3606 TGTACA
Bst1107I 370 GTATAC GTA^TAC
2658 GTATAC
BstBI 3295 TTCGAA TT^CGAA
3487 TTCGAA
BstZ17I 370 GTATAC GTA^TAC
2658 GTATAC
Bsu36I 4111 CCTCAGG CC^T[ACGT]AGG
4614 CCTGAGG
DraIII 721 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2895 CACAGTGTG
Eco81I 4111 CCTCAGG CC^T[ACGT]AGG
4614 CCTGAGG
EcoRV 2144 GATATC GAT^ATC
5393 GATATC
FbaI 1482 TGATCA T^GATCA
2765 TGATCA
HaeII 1545 AGCGCC [GA]GCGC^[CT]
3225 GGCGCT
MspA1I 3864 CAGCTG C[AC]G^C[GT]G
4246 CAGCTG
NciI 4355 CCGGG CC^[GC]GG
4398 CCGGG
NcoI 320 CCATGG C^CATGG
2663 CCATGG
NspV 3295 TTCGAA TT^CGAA
3487 TTCGAA
PstI 4237 CTGCAG CTGCA^G
5099 CTGCAG
PvuII 3864 CAGCTG CAG^CTG
4246 CAGCTG
RcaI 1527 TCATGA T^CATGA
3371 TCATGA
SfcI 4237 CTGCAG C^T[GA][CT]AG
5099 CTGCAG
SfuI 3295 TTCGAA TT^CGAA
3487 TTCGAA
SmlI 2151 CTTAAG C^T[CT][GA]AG
4231 CTCAAG
SspBI 1184 TGTACA T^GTACA
3606 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BanII
BbsI BbvCI BbvI BfaI BmyI
BpmI BpuAI BsaBI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EarI EcoNI EcoRI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PshBI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XhoII XmnI