##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AclI AcsI AocI ApoI AscI
AseI AsnI Asp700I BamHI BciVI
BclI BsiWI BsmBI Bsp1407I BsrGI
BstBI Bsu36I DraI Eco81I EcoRI
EcoRV EcoT22I FbaI HindIII HpaI
MluI NdeI NotI NruI NsiI
NspV PacI PflMI PmeI PshBI
Psp1406I PvuI SapI ScaI SexAI
SfuI SnaBI SspBI SspI SwaI
Van91I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 5421 GGTACC G^GTACC
AccIII 1895 TCCGGA T^CCGGA
AflII 422 CTTAAG C^TTAAG
AhdI 5253 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 5325 GGATC GGATC(4/5)
Asp718I 5421 GGTACC G^GTACC
AspEI 5253 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3740 TGCGCA TGC^GCA
AvrII 5434 CCTAGG C^CTAGG
BbsI 2526 GAAGAC GAAGAC(2/6)
BbvCI 5120 GCTGAGG CCTCAGC(-5/-2)
BfrI 422 CTTAAG C^TTAAG
BglII 2578 AGATCT A^GATCT
BlnI 5434 CCTAGG C^CTAGG
BpuAI 2526 GAAGAC GAAGAC(2/6)
BsaBI 1211 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1895 TCCGGA T^CCGGA
BspDI 1338 ATCGAT AT^CGAT
BspEI 1895 TCCGGA T^CCGGA
BspHI 482 TCATGA T^CATGA
BspLU11I 1293 ACATGT A^CATGT
BssSI 1560 CACGAG CACGAG(-5/-1)
Bst1107I 524 GTATAC GTA^TAC
BstEII 4914 GGTGACC G^GT[ACGT]ACC
BstPI 4914 GGTGACC G^GT[ACGT]ACC
BstYI 2578 AGATCT [GA]^GATC[CT]
BstZ17I 524 GTATAC GTA^TAC
ClaI 1338 ATCGAT AT^CGAT
DrdI 691 GACCCCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 5253 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4914 GGTGACC G^GT[ACGT]ACC
FseI 3428 GGCCGGCC GGCCGG^CC
FspI 3740 TGCGCA TGC^GCA
KpnI 5421 GGTACC GGTAC^C
MamI 1211 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3218 CAATTG C^AATTG
MflI 2578 AGATCT [GA]^GATC[CT]
MroI 1895 TCCGGA T^CCGGA
MunI 3218 CAATTG C^AATTG
NheI 1052 GCTAGC G^CTAGC
PaeR7I 2447 CTCGAG C^TCGAG
RcaI 482 TCATGA T^CATGA
SalI 1344 GTCGAC G^TCGAC
SpeI 4850 ACTAGT A^CTAGT
TfiI 5375 GATTC G^A[AT]TC
Tsp509I 3219 AATT ^AATT
XhoI 2447 CTCGAG C^TCGAG
XhoII 2578 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AatII 1692 GACGTC GACGT^C
3465 GACGTC
Alw44I 1289 GTGCAC G^TGCAC
1382 GTGCAC
ApaLI 1289 GTGCAC G^TGCAC
1382 GTGCAC
BssHII 219 GCGCGC G^CGCGC
260 GCGCGC
CpoI 112 CGGACCG CG^G[AT]CCG
540 CGGACCG
HincII 1344 GTCGAC GT[CT]^[GA]AC
4405 GTCAAC
HindII 1344 GTCGAC GT[CT]^[GA]AC
4405 GTCAAC
NcoI 1118 CCATGG C^CATGG
1256 CCATGG
PshAI 3345 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3564 GACAAGTGTC
RsrII 112 CGGACCG CG^G[AT]CCG
540 CGGACCG
SacI 2450 GAGCTC GAGCT^C
4947 GAGCTC
SbfI 15 CCTGCAGG CCTGCA^GG
3314 CCTGCAGG
SgrAI 214 CACCGGCG C[GA]^CCGG[CT]G
747 CACCGGTG
Sse8387I 15 CCTGCAGG CCTGCA^GG
3314 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AgeI AluI AlwNI Aor51HI
ApaI AspHI AspI AvaI AvaII
BalI BanI BanII BbeI BbrPI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PinAI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SfoI SmaI SmlI
SphI StuI StyI TaqI Tru9I
TseI Tsp45I TspRI Tth111I TthHB8I
XcmI XmaCI XmaI