##################################
# NO CUT ENZYMES ( 34 enzymes) #
##################################
Acc65I AclI AscI AseI AsnI
Asp718I AviII BsaI BsiWI BspDI
BstEII BstPI ClaI DraI EcoO65I
FseI FspI KpnI NdeI NheI
NotI NruI PacI PmeI PshBI
Psp1406I SbfI SexAI SfiI SgrAI
SpeI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 2547 GACGTC GACGT^C
AflII 5481 CTTAAG C^TTAAG
AgeI 3856 ACCGGT A^CCGGT
Alw44I 2561 GTGCAC G^TGCAC
ApaLI 2561 GTGCAC G^TGCAC
AspI 6387 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 6684 CCTAGG C^CTAGG
BbrPI 7427 CACGTG CAC^GTG
BbvCI 7760 GCTGAGG CCTCAGC(-5/-2)
BfrI 5481 CTTAAG C^TTAAG
BglII 1524 AGATCT A^GATCT
BlnI 6684 CCTAGG C^CTAGG
BlpI 2330 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2330 GCTAAGC GC^T[ACGT]AGC
BssHII 343 GCGCGC G^CGCGC
BssSI 2564 CACGAG CACGAG(-5/-1)
Bst1107I 1450 GTATAC GTA^TAC
BstBI 3444 TTCGAA TT^CGAA
BstZ17I 1450 GTATAC GTA^TAC
CelII 2330 GCTAAGC GC^T[ACGT]AGC
CpoI 2476 CGGTCCG CG^G[AT]CCG
DraIII 4910 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3306 GACGAGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 100 CGGCCG C^GGCCG
EclXI 100 CGGCCG C^GGCCG
Eco52I 100 CGGCCG C^GGCCG
EcoNI 2816 CCTGAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4629 GAATTC G^AATTC
EcoRV 4989 GATATC GAT^ATC
EcoT22I 4410 ATGCAT ATGCA^T
HpaI 2076 GTTAAC GTT^AAC
MluI 4381 ACGCGT A^CGCGT
NaeI 820 GCCGGC GCC^GGC
NgoMIV 820 GCCGGC G^CCGGC
NsiI 4410 ATGCAT ATGCA^T
NspV 3444 TTCGAA TT^CGAA
PflFI 6387 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3856 ACCGGT A^CCGGT
PmaCI 7427 CACGTG CAC^GTG
PmlI 7427 CACGTG CAC^GTG
PvuI 4957 CGATCG CGAT^CG
RsrII 2476 CGGTCCG CG^G[AT]CCG
SalI 5706 GTCGAC G^TCGAC
SapI 3053 GAAGAGC GCTCTTC(1/4)
SfuI 3444 TTCGAA TT^CGAA
SmaI 1218 CCCGGG CCC^GGG
SnaBI 6479 TACGTA TAC^GTA
SspI 1155 AATATT AAT^ATT
StuI 5648 AGGCCT AGG^CCT
Tth111I 6387 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1218 CCCGGG C^CCGGG
XmaI 1218 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AhdI 654 GACATGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1731 GACTTCAAGTC
ApaI 93 GGGCCC GGGCC^C
2704 GGGCCC
Asp700I 4820 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5427 GAACAGTTTC
AspEI 654 GACATGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1731 GACTTCAAGTC
BamHI 6141 GGATCC G^GATCC
6778 GGATCC
BbeI 361 GGCGCC GGCGC^C
823 GGCGCC
BcgI 2553 CGAGGGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2566 CGAGCCTGCTGC
BciVI 2246 GGATAC GTATCC(6/5)
5419 GGATAC
BclI 4516 TGATCA T^GATCA
6292 TGATCA
BsaBI 4289 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5594 GATCACCATC
BsmBI 6392 CGTCTC CGTCTC(1/5)
7695 GAGACG
BspHI 6550 TCATGA T^CATGA
7266 TCATGA
Eam1105I 654 GACATGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1731 GACTTCAAGTC
FbaI 4516 TGATCA T^GATCA
6292 TGATCA
KasI 361 GGCGCC G^GCGCC
823 GGCGCC
MamI 4289 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5594 GATCACCATC
MfeI 2883 CAATTG C^AATTG
7350 CAATTG
MunI 2883 CAATTG C^AATTG
7350 CAATTG
NarI 361 GGCGCC GG^CGCC
823 GGCGCC
RcaI 6550 TCATGA T^CATGA
7266 TCATGA
ScaI 519 AGTACT AGT^ACT
1834 AGTACT
SfoI 361 GGCGCC GGC^GCC
823 GGCGCC
XmnI 4820 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5427 GAACAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI AocI Aor51HI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PaeR7I PflMI
PleI PpuMI PshAI PstI PvuII
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoI XhoII