##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AspEI AviII
AvrII BamHI BbeI BbrPI BcgI
BlnI BsaHI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrBI BsrFI
BssHII Bst1107I BstBI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI FseI FspI
HaeII HgaI Hin1I HpaI KasI
KspI MfeI MluI MroI MspA1I
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SexAI SfoI SfuI SgrAI SnaBI
Sse8387I SspI StuI SwaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 3737 GGTACC G^GTACC
AflII 4341 CTTAAG C^TTAAG
ApaI 288 GGGCCC GGGCC^C
AseI 2708 ATTAAT AT^TAAT
AsnI 2708 ATTAAT AT^TAAT
Asp718I 3737 GGTACC G^GTACC
AspHI 2580 GTGCTC G[AT]GC[AT]^C
AspI 2453 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4112 CCCGGG C^[CT]CG[GA]G
BanII 288 GGGCCC G[GA]GC[CT]^C
BfrI 4341 CTTAAG C^TTAAG
BglI 4087 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3855 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3855 GCTTAGC GC^T[ACGT]AGC
BsaAI 826 CACGTA [CT]AC^GT[GA]
BsaBI 466 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3237 GAGACC GGTCTC(1/5)
BsiHKAI 2580 GTGCTC G[AT]GC[AT]^C
BsmBI 4065 GAGACG CGTCTC(1/5)
BsmFI 15 GTCCC GGGAC(10/14)
BsoBI 4112 CCCGGG C^[CT]CG[GA]G
BspMI 341 ACCTGC ACCTGC(4/8)
BssSI 3025 CACGAG CACGAG(-5/-1)
BstEII 2417 GGTAACC G^GT[ACGT]ACC
BstPI 2417 GGTAACC G^GT[ACGT]ACC
CelII 3855 GCTTAGC GC^T[ACGT]AGC
CfoI 3393 GCGC GCG^C
EcoO65I 2417 GGTAACC G^GT[ACGT]ACC
EcoRV 3504 GATATC GAT^ATC
HhaI 3393 GCGC GCG^C
HinP1I 3393 GCGC G^CGC
HindIII 4302 AAGCTT A^AGCTT
KpnI 3737 GGTACC GGTAC^C
MamI 466 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 2453 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 181 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2708 ATTAAT AT^TAAT
SfiI 4086 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 4112 CCCGGG CCC^GGG
SpeI 2692 ACTAGT A^CTAGT
Tth111I 2453 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 181 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3803 TCTAGA T^CTAGA
XmaCI 4112 CCCGGG C^CCGGG
XmaI 4112 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BalI 189 TGGCCA TGG^CCA
4085 TGGCCA
BanI 3737 GGTACC G^G[CT][GA]CC
4023 GGCACC
BbsI 2643 GAAGAC GAAGAC(2/6)
1018 GTCTTC
BbvCI 3994 CCTCAGC CCTCAGC(-5/-2)
4129 CCTCAGC
BciVI 176 GTATCC GTATCC(6/5)
4432 GTATCC
BglII 329 AGATCT A^GATCT
4345 AGATCT
BmyI 288 GGGCCC G[AGT]GC[ACT]^C
2580 GTGCTC
BpuAI 2643 GAAGAC GAAGAC(2/6)
1018 GTCTTC
BsgI 1671 GTGCAG GTGCAG(16/14)
1839 GTGCAG
Bsp1286I 288 GGGCCC G[AGT]GC[ACT]^C
2580 GTGCTC
BstAPI 2681 GCAGAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3522 GCATACCTTGC
BstXI 3187 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4139 CCAAAGTGCTGG
DraI 3100 TTTAAA TTT^AAA
4407 TTTAAA
EaeI 189 TGGCCA [CT]^GGCC[GA]
4085 TGGCCA
EcoT22I 536 ATGCAT ATGCA^T
2595 ATGCAT
HincII 643 GTCAAC GT[CT]^[GA]AC
2432 GTCAAC
HindII 643 GTCAAC GT[CT]^[GA]AC
2432 GTCAAC
MluNI 189 TGGCCA TGG^CCA
4085 TGGCCA
MscI 189 TGGCCA TGG^CCA
4085 TGGCCA
NcoI 409 CCATGG C^CATGG
4226 CCATGG
NsiI 536 ATGCAT ATGCA^T
2595 ATGCAT
PpuMI 27 AGGTCCT [GA]G^G[AT]CC[CT]
83 AGGACCT
PstI 475 CTGCAG CTGCA^G
737 CTGCAG
SmlI 3220 CTTGAG C^T[CT][GA]AG
4341 CTTAAG
SphI 1202 GCATGC GCATG^C
1538 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AvaII
BbvI BclI BcnI BfaI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmI Bsp1407I BspHI BspLU11I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRI
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PleI RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI