##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BglI BglII BlnI BsaHI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
BsoBI BspDI BspEI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstYI Bsu36I ClaI CpoI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRV FauI FseI FspI
HaeII Hin1I HpaI KasI KpnI
KspI MflI MluI MluNI MroI
MscI MspA1I MvnI NarI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI Psp1406I
PvuI PvuII RsrII SacI SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI XbaI
XcmI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AflII 2765 CTTAAG C^TTAAG
AflIII 370 ACATGT A^C[GA][CT]GT
AlwNI 3125 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 3462 GTGCTC G[AT]GC[AT]^C
AspI 1365 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 3089 GGGCTC G[GA]GC[CT]^C
BbvCI 1101 CCTCAGC CCTCAGC(-5/-2)
BciVI 2959 GTATCC GTATCC(6/5)
BclI 152 TGATCA T^GATCA
BfrI 2765 CTTAAG C^TTAAG
BlpI 1592 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1592 GCTGAGC GC^T[ACGT]AGC
BsaAI 2283 CACGTA [CT]AC^GT[GA]
BsaBI 2556 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 49 CGGCCG CG[GA][CT]^CG
BsiHKAI 3462 GTGCTC G[AT]GC[AT]^C
BsmFI 192 GGGAC GGGAC(10/14)
BsmI 1204 GCATTC GAATGC(1/-1)
Bsp1407I 1827 TGTACA T^GTACA
BspLU11I 370 ACATGT A^CATGT
BspMI 2059 ACCTGC ACCTGC(4/8)
BsrBI 59 GAGCGG CCGCTC(-3/-3)
BsrFI 51 GCCGGC [GA]^CCGG[CT]
BsrGI 1827 TGTACA T^GTACA
Bst1107I 1390 GTATAC GTA^TAC
BstXI 2467 CCAGTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1390 GTATAC GTA^TAC
CelII 1592 GCTGAGC GC^T[ACGT]AGC
CfoI 70 GCGC GCG^C
Cfr10I 51 GCCGGC [GA]^CCGG[CT]
DsaI 8 CCATGG C^C[GA][CT]GG
EagI 49 CGGCCG C^GGCCG
EclXI 49 CGGCCG C^GGCCG
Eco52I 49 CGGCCG C^GGCCG
EcoT22I 4062 ATGCAT ATGCA^T
FbaI 152 TGATCA T^GATCA
HgaI 2151 GACGC GACGC(5/10)
HhaI 70 GCGC GCG^C
HinP1I 70 GCGC G^CGC
HincII 2835 GTCGAC GT[CT]^[GA]AC
HindII 2835 GTCGAC GT[CT]^[GA]AC
MamI 2556 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 51 GCCGGC GCC^GGC
NcoI 8 CCATGG C^CATGG
NgoMIV 51 GCCGGC G^CCGGC
NsiI 4062 ATGCAT ATGCA^T
PflFI 1365 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1137 CCATCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2056 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 829 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2903 CTGCAG CTGCA^G
SalI 2835 GTCGAC G^TCGAC
SspBI 1827 TGTACA T^GTACA
SwaI 4226 ATTTAAAT ATTT^AAAT
Tth111I 1365 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1137 CCATCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Asp700I 885 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3064 GAAGTTTTTC
BcnI 2278 CCCGG CC^[GC]GG
2364 CCCGG
BfaI 103 CTAG C^TAG
1464 CTAG
BmyI 3089 GGGCTC G[AGT]GC[ACT]^C
3462 GTGCTC
Bsp1286I 3089 GGGCTC G[AGT]GC[ACT]^C
3462 GTGCTC
BspHI 155 TCATGA T^CATGA
2219 TCATGA
BsrDI 1237 CATTGC GCAATG(2/0)
2538 CATTGC
Cac8I 51 GCCGGC GC[ACGT]^[ACGT]GC
1106 GCCTGC
DraII 2056 AGGACCT [GA]G^G[ACGT]CC[CT]
2984 AGGCCCT
EaeI 5 CGGCCA [CT]^GGCC[GA]
49 CGGCCG
EcoO109I 2056 AGGACCT [GA]G^G[ACGT]CC[CT]
2984 AGGCCCT
EcoT14I 8 CCATGG C^C[AT][AT]GG
584 CCAAGG
HindIII 94 AAGCTT A^AGCTT
3613 AAGCTT
HphI 288 GGTGA GGTGA(8/7)
2799 TCACC
MaeI 103 CTAG C^TAG
1464 CTAG
MfeI 358 CAATTG C^AATTG
895 CAATTG
MunI 358 CAATTG C^AATTG
895 CAATTG
NciI 2278 CCCGG CC^[GC]GG
2364 CCCGG
NdeI 307 CATATG CA^TATG
2603 CATATG
NspI 370 ACATGT [GA]CATG^[CT]
1119 GCATGT
RcaI 155 TCATGA T^CATGA
2219 TCATGA
SmlI 1318 CTCAAG C^T[CT][GA]AG
2765 CTTAAG
StyI 8 CCATGG C^C[AT][AT]GG
584 CCAAGG
XmnI 885 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3064 GAAGTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AvaII
BbsI BbvI BpmI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraI EarI EcoRI EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I