##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I Aor51HI ApaLI Asp718I AviII
AvrII BfrI BlnI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraI
Eco47III EcoRI EcoRV EcoT22I FseI
FspI HpaI KpnI MfeI MroI
MunI NheI NotI NruI NsiI
NspI NspV PinAI PmeI PshAI
Psp1406I PvuI RsrII SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 740 GACGTC GACGT^C
AccI 324 GTCGAC GT^[AC][GT]AC
AcsI 3077 GAATTT [GA]^AATT[CT]
AfaI 3350 GTAC GT^AC
AhdI 452 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1617 CCTTAGG CC^T[ACGT]AGG
ApoI 3077 GAATTT [GA]^AATT[CT]
AscI 1683 GGCGCGCC GG^CGCGCC
Asp700I 1890 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 452 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1934 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 831 GCAAGGGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1752 GTATCC GTATCC(6/5)
BsaBI 2537 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1325 GTGCAG GTGCAG(16/14)
BsmBI 2654 CGTCTC CGTCTC(1/5)
BspHI 2782 TCATGA T^CATGA
BstAPI 1327 GCAGCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3131 GGTCACC G^GT[ACGT]ACC
BstPI 3131 GGTCACC G^GT[ACGT]ACC
Bsu36I 1617 CCTTAGG CC^T[ACGT]AGG
DraIII 2296 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3382 GACACCGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 452 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1617 CCTTAGG CC^T[ACGT]AGG
EcoO65I 3131 GGTCACC G^GT[ACGT]ACC
HindIII 3617 AAGCTT A^AGCTT
KspI 1480 CCGCGG CCGC^GG
MamI 2537 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1351 ACGCGT A^CGCGT
NcoI 63 CCATGG C^CATGG
NdeI 2190 CATATG CA^TATG
PacI 2239 TTAATTAA TTAAT^TAA
PaeR7I 1012 CTCGAG C^TCGAG
PflFI 1934 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 173 CTGCAG CTGCA^G
RcaI 2782 TCATGA T^CATGA
RsaI 3350 GTAC GT^AC
SacII 1480 CCGCGG CCGC^GG
SalI 324 GTCGAC G^TCGAC
SapI 2276 GCTCTTC GCTCTTC(1/4)
SexAI 2427 ACCTGGT A^CC[AT]GGT
Tth111I 1934 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3325 TCTAGA T^CTAGA
XhoI 1012 CTCGAG C^TCGAG
XmnI 1890 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 110 ACGTGT A^C[GA][CT]GT
1351 ACGCGT
AseI 2199 ATTAAT AT^TAAT
2242 ATTAAT
AsnI 2199 ATTAAT AT^TAAT
2242 ATTAAT
BalI 3027 TGGCCA TGG^CCA
3458 TGGCCA
BbeI 406 GGCGCC GGCGC^C
1850 GGCGCC
BbrPI 109 CACGTG CAC^GTG
1273 CACGTG
BbsI 16 GAAGAC GAAGAC(2/6)
1915 GAAGAC
BbvCI 1493 GCTGAGG CCTCAGC(-5/-2)
1793 GCTGAGG
BclI 2441 TGATCA T^GATCA
2785 TGATCA
BglII 1038 AGATCT A^GATCT
2670 AGATCT
BlpI 1801 GCTCAGC GC^T[ACGT]AGC
2309 GCTGAGC
Bpu1102I 1801 GCTCAGC GC^T[ACGT]AGC
2309 GCTGAGC
BpuAI 16 GAAGAC GAAGAC(2/6)
1915 GAAGAC
BsaWI 1130 ACCGGA [AT]^CCGG[AT]
1171 ACCGGA
CelII 1801 GCTCAGC GC^T[ACGT]AGC
2309 GCTGAGC
EcoNI 205 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1876 CCTGCCCGAGG
FbaI 2441 TGATCA T^GATCA
2785 TGATCA
HincII 324 GTCGAC GT[CT]^[GA]AC
2570 GTTGAC
HindII 324 GTCGAC GT[CT]^[GA]AC
2570 GTTGAC
KasI 406 GGCGCC G^GCGCC
1850 GGCGCC
MluNI 3027 TGGCCA TGG^CCA
3458 TGGCCA
MscI 3027 TGGCCA TGG^CCA
3458 TGGCCA
NarI 406 GGCGCC GG^CGCC
1850 GGCGCC
PmaCI 109 CACGTG CAC^GTG
1273 CACGTG
PmlI 109 CACGTG CAC^GTG
1273 CACGTG
PshBI 2199 ATTAAT AT^TAAT
2242 ATTAAT
PvuII 365 CAGCTG CAG^CTG
672 CAGCTG
SfoI 406 GGCGCC GGC^GCC
1850 GGCGCC
SmlI 1004 CTCAAG C^T[CT][GA]AG
1012 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BamHI BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI