##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI AspEI AspI AvrII BanII
BcgI BglI BlnI BsaBI BsaWI
BseAI BsiEI BsiWI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstBI
BstEII BstPI BstXI Bsu36I Cfr10I
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI FseI KspI
MamI MluI MroI NaeI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 3516 GGTACC G^GTACC
AccI 3977 GTATAC GT^[AC][GT]AC
AccII 213 CGCG CG^CG
AflII 3436 CTTAAG C^TTAAG
Alw44I 3096 GTGCAC G^TGCAC
ApaLI 3096 GTGCAC G^TGCAC
Asp700I 3125 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3516 GGTACC G^GTACC
AspHI 3096 GTGCAC G[AT]GC[AT]^C
AviII 2180 TGCGCA TGC^GCA
BamHI 1868 GGATCC G^GATCC
BbeI 1851 GGCGCC GGCGC^C
BbrPI 3082 CACGTG CAC^GTG
BbvCI 1047 GCTGAGG CCTCAGC(-5/-2)
BciVI 1395 GGATAC GTATCC(6/5)
BclI 3314 TGATCA T^GATCA
BfrI 3436 CTTAAG C^TTAAG
BglII 3406 AGATCT A^GATCT
BlpI 1764 GCTCAGC GC^T[ACGT]AGC
BmyI 3096 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 1764 GCTCAGC GC^T[ACGT]AGC
BsaAI 3082 CACGTG [CT]AC^GT[GA]
BsiHKAI 3096 GTGCAC G[AT]GC[AT]^C
BsmBI 1860 GAGACG CGTCTC(1/5)
BsmI 64 GCATTC GAATGC(1/-1)
Bsp1286I 3096 GTGCAC G[AGT]GC[ACT]^C
BspDI 2520 ATCGAT AT^CGAT
Bst1107I 3977 GTATAC GTA^TAC
BstUI 213 CGCG CG^CG
BstZ17I 3977 GTATAC GTA^TAC
CelII 1764 GCTCAGC GC^T[ACGT]AGC
ClaI 2520 ATCGAT AT^CGAT
DraI 3234 TTTAAA TTT^AAA
FbaI 3314 TGATCA T^GATCA
FspI 2180 TGCGCA TGC^GCA
HaeII 1851 GGCGCC [GA]GCGC^[CT]
HindIII 2368 AAGCTT A^AGCTT
HpaI 2478 GTTAAC GTT^AAC
KasI 1851 GGCGCC G^GCGCC
KpnI 3516 GGTACC GGTAC^C
MvnI 213 CGCG CG^CG
NarI 1851 GGCGCC GG^CGCC
NdeI 1651 CATATG CA^TATG
PmaCI 3082 CACGTG CAC^GTG
PmlI 3082 CACGTG CAC^GTG
SapI 3961 GAAGAGC GCTCTTC(1/4)
SfoI 1851 GGCGCC GGC^GCC
XmnI 3125 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 529 GACGCC G[GA]^CG[CT]C
1851 GGCGCC
BcnI 854 CCGGG CC^[GC]GG
3074 CCCGG
BsaHI 529 GACGCC G[GA]^CG[CT]C
1851 GGCGCC
BsaI 1160 GAGACC GGTCTC(1/5)
2554 GAGACC
BseRI 2442 GAGGAG GAGGAG(10/8)
2796 GAGGAG
BsmFI 2202 GGGAC GGGAC(10/14)
1365 GTCCC
Bsp1407I 73 TGTACA T^GTACA
3901 TGTACA
BspLU11I 131 ACATGT A^CATGT
2288 ACATGT
BsrGI 73 TGTACA T^GTACA
3901 TGTACA
BstAPI 1628 GCACACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3207 GCAAATTTTGC
DraII 2202 GGGACCT [GA]G^G[ACGT]CC[CT]
3689 GGGTCCT
EcoO109I 2202 GGGACCT [GA]G^G[ACGT]CC[CT]
3689 GGGTCCT
EcoRV 768 GATATC GAT^ATC
3727 GATATC
EcoT22I 693 ATGCAT ATGCA^T
1075 ATGCAT
FauI 317 CCCGC CCCGC(4/6)
975 CCCGC
HapII 854 CCGG C^CGG
3075 CCGG
HgaI 529 GACGC GACGC(5/10)
2085 GCGTC
Hin1I 529 GACGCC G[GA]^CG[CT]C
1851 GGCGCC
HpaII 854 CCGG C^CGG
3075 CCGG
MspI 854 CCGG C^CGG
3075 CCGG
NciI 854 CCGGG CC^[GC]GG
3074 CCCGG
NsiI 693 ATGCAT ATGCA^T
1075 ATGCAT
PflMI 533 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2699 CCATATCTTGG
PpuMI 2202 GGGACCT [GA]G^G[AT]CC[CT]
3689 GGGTCCT
SphI 1113 GCATGC GCATG^C
3392 GCATGC
SspBI 73 TGTACA T^GTACA
3901 TGTACA
StuI 2333 AGGCCT AGG^CCT
2980 AGGCCT
Van91I 533 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2699 CCATATCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AvaI AvaII
BalI BanI BbsI BbvI BfaI
BpmI BpuAI BsaJI BsgI BsiYI
BslI BsmAI BsoBI BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MunI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII