##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI AspEI BamHI
BbeI BbvCI BcgI BclI BfrI
BsaBI BsaHI BseAI BsiWI BspEI
BssHII BstAPI BstBI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI FbaI FseI HaeII HgaI
Hin1I KasI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 2316 GGTACC G^GTACC
AflIII 1506 ACATGT A^C[GA][CT]GT
AgeI 1917 ACCGGT A^CCGGT
AocI 1525 CCTGAGG CC^T[ACGT]AGG
Asp718I 2316 GGTACC G^GTACC
AspI 3921 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3092 TGCGCA TGC^GCA
AvrII 3735 CCTAGG C^CTAGG
BbrPI 1951 CACGTG CAC^GTG
BbsI 3954 GAAGAC GAAGAC(2/6)
BlnI 3735 CCTAGG C^CTAGG
BlpI 2240 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2240 GCTGAGC GC^T[ACGT]AGC
BpuAI 3954 GAAGAC GAAGAC(2/6)
BsaAI 1951 CACGTG [CT]AC^GT[GA]
BsaWI 1917 ACCGGT [AT]^CCGG[AT]
BsiEI 2154 CGACCG CG[GA][CT]^CG
BspDI 1945 ATCGAT AT^CGAT
BspHI 555 TCATGA T^CATGA
BspLU11I 1506 ACATGT A^CATGT
BsrBI 1375 CCGCTC CCGCTC(-3/-3)
BsrDI 277 CATTGC GCAATG(2/0)
Bst1107I 4012 GTATAC GTA^TAC
BstEII 97 GGTGACC G^GT[ACGT]ACC
BstPI 97 GGTGACC G^GT[ACGT]ACC
BstZ17I 4012 GTATAC GTA^TAC
Bsu36I 1525 CCTGAGG CC^T[ACGT]AGG
CelII 2240 GCTGAGC GC^T[ACGT]AGC
ClaI 1945 ATCGAT AT^CGAT
DraIII 2756 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1525 CCTGAGG CC^T[ACGT]AGG
EcoO65I 97 GGTGACC G^GT[ACGT]ACC
EcoRV 1545 GATATC GAT^ATC
FspI 3092 TGCGCA TGC^GCA
HpaI 3477 GTTAAC GTT^AAC
KpnI 2316 GGTACC GGTAC^C
KspI 93 CCGCGG CCGC^GG
PaeR7I 446 CTCGAG C^TCGAG
PflFI 3921 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2401 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1917 ACCGGT A^CCGGT
PmaCI 1951 CACGTG CAC^GTG
PmlI 1951 CACGTG CAC^GTG
RcaI 555 TCATGA T^CATGA
SacI 2351 GAGCTC GAGCT^C
SacII 93 CCGCGG CCGC^GG
ScaI 1769 AGTACT AGT^ACT
Tth111I 3921 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2401 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 446 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspHI 2351 GAGCTC G[AT]GC[AT]^C
3288 GTGCTC
AvaI 446 CTCGAG C^[CT]CG[GA]G
1566 CCCGAG
BciVI 3693 GGATAC GTATCC(6/5)
2091 GTATCC
BglI 152 GCCTTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1020 GCCGCAGAGGC
BglII 2289 AGATCT A^GATCT
3971 AGATCT
BsiHKAI 2351 GAGCTC G[AT]GC[AT]^C
3288 GTGCTC
BsmI 2605 GAATGC GAATGC(1/-1)
2508 GCATTC
BsoBI 446 CTCGAG C^[CT]CG[GA]G
1566 CCCGAG
BspMI 2465 ACCTGC ACCTGC(4/8)
3 GCAGGT
BsrFI 1917 ACCGGT [GA]^CCGG[CT]
3340 GCCGGT
BssSI 700 CACGAG CACGAG(-5/-1)
653 CTCGTG
BstXI 1620 CCACAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2105 CCAGGGCCCTGG
Cfr10I 1917 ACCGGT [GA]^CCGG[CT]
3340 GCCGGT
DraI 710 TTTAAA TTT^AAA
3775 TTTAAA
EcoNI 1346 CCTAAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2575 CCTGAGCAAGG
EcoT22I 2306 ATGCAT ATGCA^T
2607 ATGCAT
HindIII 1889 AAGCTT A^AGCTT
3104 AAGCTT
NcoI 132 CCATGG C^CATGG
892 CCATGG
NsiI 2306 ATGCAT ATGCA^T
2607 ATGCAT
PvuII 842 CAGCTG CAG^CTG
3364 CAGCTG
SapI 782 GAAGAGC GCTCTTC(1/4)
387 GCTCTTC
SphI 2038 GCATGC GCATG^C
2308 GCATGC
SspI 2528 AATATT AAT^ATT
2858 AATATT
StuI 105 AGGCCT AGG^CCT
2790 AGGCCT
XcmI 123 CCAATAGAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2641 CCAGGAAGCCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApaI ApoI Asp700I
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI