##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccIII AclI AflIII AgeI Alw44I
Aor51HI ApaLI AscI AviII BbeI
BbrPI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BspLU11I BsrDI BssHII
BssSI BstBI BstEII BstPI ClaI
CpoI DraI DraIII Eco47III EcoO65I
EcoRI EcoT22I FspI HindIII HpaI
KasI KspI MluI MroI NarI
NdeI NheI NotI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 2103 GACGTC GACGT^C
Acc65I 872 GGTACC G^GTACC
AcsI 1924 GAATTT [GA]^AATT[CT]
AflII 2481 CTTAAG C^TTAAG
AhdI 2905 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1924 GAATTT [GA]^AATT[CT]
AseI 3878 ATTAAT AT^TAAT
AsnI 3878 ATTAAT AT^TAAT
Asp700I 3494 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 872 GGTACC G^GTACC
AspEI 2905 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2181 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1621 CCCGGG C^[CT]CG[GA]G
BamHI 397 GGATCC G^GATCC
BcgI 3446 GCAGATCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3431 GGATAC GTATCC(6/5)
BfrI 2481 CTTAAG C^TTAAG
BsaAI 1485 TACGTG [CT]AC^GT[GA]
BsaBI 2153 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1454 GAATGC GAATGC(1/-1)
BsoBI 1621 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2863 TGTACA T^GTACA
BsrBI 666 GAGCGG CCGCTC(-3/-3)
BsrGI 2863 TGTACA T^GTACA
Bst1107I 1803 GTATAC GTA^TAC
BstZ17I 1803 GTATAC GTA^TAC
DrdI 2226 GACCACCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1 CGGCCG C^GGCCG
Eam1105I 2905 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1 CGGCCG C^GGCCG
Eco52I 1 CGGCCG C^GGCCG
EcoNI 3050 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3321 GATATC GAT^ATC
FseI 2 GGCCGGCC GGCCGG^CC
KpnI 872 GGTACC GGTAC^C
MamI 2153 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2950 CAATTG C^AATTG
MunI 2950 CAATTG C^AATTG
NaeI 3 GCCGGC GCC^GGC
NgoMIV 3 GCCGGC G^CCGGC
NruI 3455 TCGCGA TCG^CGA
PflFI 2181 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2616 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3878 ATTAAT AT^TAAT
ScaI 886 AGTACT AGT^ACT
SexAI 3392 ACCTGGT A^CC[AT]GGT
SmaI 1621 CCCGGG CCC^GGG
SphI 2267 GCATGC GCATG^C
SspBI 2863 TGTACA T^GTACA
Tth111I 2181 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1621 CCCGGG C^CCGGG
XmaI 1621 CCCGGG C^CCGGG
XmnI 3494 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1803 GTATAC GT^[AC][GT]AC
1827 GTCTAC
AcyI 177 GACGCC G[GA]^CG[CT]C
2103 GACGTC
AocI 1182 CCTGAGG CC^T[ACGT]AGG
3147 CCTGAGG
AvrII 2273 CCTAGG C^CTAGG
3792 CCTAGG
BbvCI 3413 CCTCAGC CCTCAGC(-5/-2)
1116 GCTGAGG
BclI 2239 TGATCA T^GATCA
2636 TGATCA
BglII 916 AGATCT A^GATCT
2320 AGATCT
BlnI 2273 CCTAGG C^CTAGG
3792 CCTAGG
BlpI 1988 GCTCAGC GC^T[ACGT]AGC
2735 GCTGAGC
Bpu1102I 1988 GCTCAGC GC^T[ACGT]AGC
2735 GCTGAGC
BsaHI 177 GACGCC G[GA]^CG[CT]C
2103 GACGTC
BsiEI 1 CGGCCG CG[GA][CT]^CG
3245 CGGTCG
BsrFI 3 GCCGGC [GA]^CCGG[CT]
1819 ACCGGC
BstAPI 851 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1544 GCAGGCAGTGC
Bsu36I 1182 CCTGAGG CC^T[ACGT]AGG
3147 CCTGAGG
CelII 1988 GCTCAGC GC^T[ACGT]AGC
2735 GCTGAGC
Cfr10I 3 GCCGGC [GA]^CCGG[CT]
1819 ACCGGC
Eco81I 1182 CCTGAGG CC^T[ACGT]AGG
3147 CCTGAGG
FbaI 2239 TGATCA T^GATCA
2636 TGATCA
Hin1I 177 GACGCC G[GA]^CG[CT]C
2103 GACGTC
HincII 1671 GTCAAC GT[CT]^[GA]AC
2106 GTCAAC
HindII 1671 GTCAAC GT[CT]^[GA]AC
2106 GTCAAC
PpuMI 102 GGGACCT [GA]G^G[AT]CC[CT]
2678 GGGACCC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII