##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        AspEI       AspI        
AviII       BbeI        BbrPI       BcgI        BglI        
BglII       BsaAI       BsaHI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BspDI       BspEI       
BspMI       BssHII      BstEII      BstPI       BstUI       
CfoI        ClaI        CpoI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoT22I     FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      KasI        KspI        
MluI        MroI        MvnI        NarI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        SfiI        SfoI        
SgrAI       SnaBI       SphI        Sse8387I    StuI        
SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I       4223  GGTACC               G^GTACC
AccI         2417  GTATAC               GT^[AC][GT]AC
AflII        4155  CTTAAG               C^TTAAG
ApaI          884  GGGCCC               GGGCC^C
Asp718I      4223  GGTACC               G^GTACC
AvaI         2623  CCCGGG               C^[CT]CG[GA]G
BamHI        2395  GGATCC               G^GATCC
BbvCI        2288  CCTCAGC              CCTCAGC(-5/-2)
BciVI         456  GTATCC               GTATCC(6/5)
BclI          863  TGATCA               T^GATCA
BfrI         4155  CTTAAG               C^TTAAG
BsaBI         858  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        3935  ACCGGA               [AT]^CCGG[AT]
BseRI          36  GAGGAG               GAGGAG(10/8)
BsoBI        2623  CCCGGG               C^[CT]CG[GA]G
BspHI        4616  TCATGA               T^CATGA
BsrDI        1574  CATTGC               GCAATG(2/0)
BsrFI          98  GCCGGC               [GA]^CCGG[CT]
Bst1107I     2417  GTATAC               GTA^TAC
BstAPI       1613  GCAGTTTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2869  TTCGAA               TT^CGAA
BstZ17I      2417  GTATAC               GTA^TAC
Cfr10I         98  GCCGGC               [GA]^CCGG[CT]
DraII         755  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DrdI         3774  GACTGATTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      755  AGGTCCC              [GA]G^G[ACGT]CC[CT]
FbaI          863  TGATCA               T^GATCA
HpaI         4480  GTTAAC               GTT^AAC
KpnI         4223  GGTACC               GGTAC^C
MamI          858  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI           98  GCCGGC               GCC^GGC
NcoI         1396  CCATGG               C^CATGG
NdeI         2330  CATATG               CA^TATG
NgoMIV         98  GCCGGC               G^CCGGC
NheI         1148  GCTAGC               G^CTAGC
NspV         2869  TTCGAA               TT^CGAA
PflMI        2085  CCAATTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         755  AGGTCCC              [GA]G^G[AT]CC[CT]
PstI          163  CTGCAG               CTGCA^G
RcaI         4616  TCATGA               T^CATGA
SfuI         2869  TTCGAA               TT^CGAA
SmaI         2623  CCCGGG               CCC^GGG
Van91I       2085  CCAATTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2839  TCTAGA               T^CTAGA
XmaCI        2623  CCCGGG               C^CCGGG
XmaI         2623  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AflIII       1921  ACATGT               A^C[GA][CT]GT
             3753  ACATGT              
AlwNI        3770  CAGGGACTG            CAG[ACGT][ACGT][ACGT]^CTG
             4695  CAGTTCCTG           
AocI         2169  CCTAAGG              CC^T[ACGT]AGG
             3794  CCTCAGG             
Asp700I       720  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2712  GAAGGATTTC          
AvrII         620  CCTAGG               C^CTAGG
             3626  CCTAGG              
BalI         2572  TGGCCA               TGG^CCA
             3309  TGGCCA              
BanI          110  GGCACC               G^G[CT][GA]CC
             4223  GGTACC              
BlnI          620  CCTAGG               C^CTAGG
             3626  CCTAGG              
BlpI           53  GCTCAGC              GC^T[ACGT]AGC
              351  GCTGAGC             
Bpu1102I       53  GCTCAGC              GC^T[ACGT]AGC
              351  GCTGAGC             
BspLU11I     1921  ACATGT               A^CATGT
             3753  ACATGT              
BsrBI          39  GAGCGG               CCGCTC(-3/-3)
              145  GAGCGG              
BssSI         382  CACGAG               CACGAG(-5/-1)
             1831  CACGAG              
BstYI         900  AGATCC               [GA]^GATC[CT]
             2395  GGATCC              
Bsu36I       2169  CCTAAGG              CC^T[ACGT]AGG
             3794  CCTCAGG             
CelII          53  GCTCAGC              GC^T[ACGT]AGC
              351  GCTGAGC             
DraI         1271  TTTAAA               TTT^AAA
             3610  TTTAAA              
DraIII       1213  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2798  CACCTTGTG           
Eco81I       2169  CCTAAGG              CC^T[ACGT]AGG
             3794  CCTCAGG             
EcoRI        2662  GAATTC               G^AATTC
             4492  GAATTC              
EcoRV         429  GATATC               GAT^ATC
             1941  GATATC              
HincII       2762  GTTGAC               GT[CT]^[GA]AC
             4480  GTTAAC              
HindII       2762  GTTGAC               GT[CT]^[GA]AC
             4480  GTTAAC              
HindIII       723  AAGCTT               A^AGCTT
             3947  AAGCTT              
MflI          900  AGATCC               [GA]^GATC[CT]
             2395  GGATCC              
MluNI        2572  TGGCCA               TGG^CCA
             3309  TGGCCA              
MscI         2572  TGGCCA               TGG^CCA
             3309  TGGCCA              
MspA1I       4104  CAGCTG               C[AC]G^C[GT]G
             4406  CAGCTG              
PvuII        4104  CAGCTG               CAG^CTG
             4406  CAGCTG              
ScaI         1056  AGTACT               AGT^ACT
             2492  AGTACT              
SexAI         481  ACCAGGT              A^CC[AT]GGT
              993  ACCTGGT             
SpeI         1862  ACTAGT               A^CTAGT
             4439  ACTAGT              
SspI         3720  AATATT               AAT^ATT
             4647  AATATT              
XcmI         1396  CCATGGACATGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2575  CCAAAAGCATGATGG     
XhoII         900  AGATCC               [GA]^GATC[CT]
             2395  GGATCC              
XmnI          720  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2712  GAAGGATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
Bsp1407I    BsrGI       BsrI        BssKI       BstF5I      
BstNI       BstXI       Cac8I       Cfr13I      DdeI        
DpnII       DsaI        EaeI        EarI        EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MfeI        MnlI        MseI        MslI        
MspI        MunI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspBI       StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I