##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AgeI        AhdI        Aor51HI     ApaI        AscI        
Asp718I     AspEI       AspI        AviII       AvrII       
BbeI        BbrPI       BglII       BlnI        BsaHI       
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspHI       BsrBI       BsrFI       BsrGI       BssHII      
BstBI       BstEII      BstPI       BstUI       Cfr10I      
ClaI        CpoI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
EcoT22I     FseI        FspI        Hin1I       HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SspBI       StuI        SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AccIII        803  TCCGGA               T^CCGGA
AflII        1204  CTTAAG               C^TTAAG
AflIII        256  ACATGT               A^C[GA][CT]GT
Alw44I       1618  GTGCAC               G^TGCAC
ApaLI        1618  GTGCAC               G^TGCAC
AseI          682  ATTAAT               AT^TAAT
AsnI          682  ATTAAT               AT^TAAT
AvaI           16  CCCGGG               C^[CT]CG[GA]G
BalI         3856  TGGCCA               TGG^CCA
BamHI        2586  GGATCC               G^GATCC
BanI         2030  GGCACC               G^G[CT][GA]CC
BbvCI         610  CCTCAGC              CCTCAGC(-5/-2)
BcgI          352  CGACAGGTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1494  GGATAC               GTATCC(6/5)
BfrI         1204  CTTAAG               C^TTAAG
BglI         2302  GCCAAAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         3181  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3181  GCTGAGC              GC^T[ACGT]AGC
BsaAI        3951  CACGTA               [CT]AC^GT[GA]
BsaBI         709  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         803  TCCGGA               [AT]^CCGG[AT]
BseAI         803  TCCGGA               T^CCGGA
BsmBI        1700  CGTCTC               CGTCTC(1/5)
BsoBI          16  CCCGGG               C^[CT]CG[GA]G
BspEI         803  TCCGGA               T^CCGGA
BspLU11I      256  ACATGT               A^CATGT
BsrDI        2250  CATTGC               GCAATG(2/0)
BstAPI       1611  GCAGGAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        2681  CCAAAAAACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        3181  GCTGAGC              GC^T[ACGT]AGC
DsaI         4150  CCATGG               C^C[GA][CT]GG
EaeI         3856  TGGCCA               [CT]^GGCC[GA]
EcoRI        3395  GAATTC               G^AATTC
FauI         2328  CCCGC                CCCGC(4/6)
HaeII         341  GGCGCT               [GA]GCGC^[CT]
HgaI         1629  GACGC                GACGC(5/10)
HincII       1858  GTTGAC               GT[CT]^[GA]AC
HindII       1858  GTTGAC               GT[CT]^[GA]AC
HindIII      3167  AAGCTT               A^AGCTT
MamI          709  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        3856  TGGCCA               TGG^CCA
MroI          803  TCCGGA               T^CCGGA
MscI         3856  TGGCCA               TGG^CCA
MslI         2007  CACTGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI         4150  CCATGG               C^CATGG
NheI          556  GCTAGC               G^CTAGC
PflMI        2064  CCAGAAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         795  GACTTCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         682  ATTAAT               AT^TAAT
SacI         1674  GAGCTC               GAGCT^C
SapI         3262  GAAGAGC              GCTCTTC(1/4)
SbfI         1588  CCTGCAGG             CCTGCA^GG
ScaI         3435  AGTACT               AGT^ACT
SmaI           16  CCCGGG               CCC^GGG
SphI          212  GCATGC               GCATG^C
Sse8387I     1588  CCTGCAGG             CCTGCA^GG
TaqI          351  TCGA                 T^CGA
TthHB8I       351  TCGA                 T^CGA
Van91I       2064  CCAGAAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2742  TCTAGA               T^CTAGA
XcmI         3525  CCAGTATTTGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          16  CCCGGG               C^CCGGG
XmaI           16  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AccI         2766  GTATAC               GT^[AC][GT]AC
             3662  GTATAC              
AlwNI         962  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2351  CAGTCACTG           
BanII         952  GAGCCC               G[GA]GC[CT]^C
             1674  GAGCTC              
BsaI         1846  GAGACC               GGTCTC(1/5)
             1891  GAGACC              
BseRI        1404  CTCCTC               GAGGAG(10/8)
             1582  GAGGAG              
BssSI         129  CACGAG               CACGAG(-5/-1)
             1708  CACGAG              
Bst1107I     2766  GTATAC               GTA^TAC
             3662  GTATAC              
BstYI        1277  AGATCC               [GA]^GATC[CT]
             2586  GGATCC              
BstZ17I      2766  GTATAC               GTA^TAC
             3662  GTATAC              
CfoI          342  GCGC                 GCG^C
              452  GCGC                
DrdI         2344  GACAAATCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3886  GACCCACCTGTC        
EcoNI          35  CCTACCTTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              217  CCTAATCAAGG         
HhaI          342  GCGC                 GCG^C
              452  GCGC                
HinP1I        342  GCGC                 G^CGC
              452  GCGC                
MflI         1277  AGATCC               [GA]^GATC[CT]
             2586  GGATCC              
SspI         3031  AATATT               AAT^ATT
             3586  AATATT              
XhoII        1277  AGATCC               [GA]^GATC[CT]
             2586  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AocI        ApoI        Asp700I     AspHI       AvaII       
BbsI        BbvI        BclI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       Bsu36I      Cac8I       Cfr13I      DdeI        
DpnII       DraI        DraII       EarI        Eco81I      
EcoO109I    EcoRII      EcoT14I     FbaI        Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XmnI