##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccII AclI AflII AgeI Aor51HI
AscI AseI AsnI AviII BamHI
BbrPI BcgI BfrI BglII BlpI
Bpu1102I BsaAI BsiEI BsiWI Bsp1407I
BspDI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I CelII
ClaI DraI DraIII EagI EclXI
Eco47III Eco52I FseI FspI KspI
MfeI MluI MunI MvnI NaeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
StuI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 1044 GACGTC GACGT^C
AccI 1539 GTCTAC GT^[AC][GT]AC
AccIII 3006 TCCGGA T^CCGGA
AflIII 5714 ACATGT A^C[GA][CT]GT
Alw44I 1803 GTGCAC G^TGCAC
AocI 1910 CCTCAGG CC^T[ACGT]AGG
ApaLI 1803 GTGCAC G^TGCAC
AspI 3467 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3551 CCTAGG C^CTAGG
BbeI 3183 GGCGCC GGCGC^C
BbvCI 2 GCTGAGG CCTCAGC(-5/-2)
BciVI 4685 GTATCC GTATCC(6/5)
BclI 2302 TGATCA T^GATCA
BglI 259 GCCTGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3551 CCTAGG C^CTAGG
BsaBI 1821 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3006 TCCGGA [AT]^CCGG[AT]
BseAI 3006 TCCGGA T^CCGGA
BsmBI 4169 GAGACG CGTCTC(1/5)
BspEI 3006 TCCGGA T^CCGGA
BspLU11I 5714 ACATGT A^CATGT
BstEII 1091 GGTTACC G^GT[ACGT]ACC
BstPI 1091 GGTTACC G^GT[ACGT]ACC
Bsu36I 1910 CCTCAGG CC^T[ACGT]AGG
CpoI 38 CGGACCG CG^G[AT]CCG
Eco81I 1910 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1091 GGTTACC G^GT[ACGT]ACC
EcoRV 2033 GATATC GAT^ATC
FbaI 2302 TGATCA T^GATCA
KasI 3183 GGCGCC G^GCGCC
MamI 1821 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3006 TCCGGA T^CCGGA
NarI 3183 GGCGCC GG^CGCC
PaeR7I 4680 CTCGAG C^TCGAG
PflFI 3467 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2345 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII 38 CGGACCG CG^G[AT]CCG
SexAI 1426 ACCAGGT A^CC[AT]GGT
SfoI 3183 GGCGCC GGC^GCC
SmaI 2841 CCCGGG CCC^GGG
SphI 4902 GCATGC GCATG^C
SspI 4997 AATATT AAT^ATT
Tth111I 3467 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2345 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3114 CCACGCTGTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4680 CTCGAG C^TCGAG
XmaCI 2841 CCCGGG C^CCGGG
XmaI 2841 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 469 GGTACC G^GTACC
3278 GGTACC
AcyI 1044 GACGTC G[GA]^CG[CT]C
3183 GGCGCC
ApaI 2838 GGGCCC GGGCC^C
5035 GGGCCC
Asp718I 469 GGTACC G^GTACC
3278 GGTACC
AvaI 2841 CCCGGG C^[CT]CG[GA]G
4680 CTCGAG
BalI 4206 TGGCCA TGG^CCA
4648 TGGCCA
BsaHI 1044 GACGTC G[GA]^CG[CT]C
3183 GGCGCC
BsgI 3398 CTGCAC GTGCAG(16/14)
4061 GTGCAG
BsmI 1205 GCATTC GAATGC(1/-1)
3388 GCATTC
BsoBI 2841 CCCGGG C^[CT]CG[GA]G
4680 CTCGAG
BspHI 2037 TCATGA T^CATGA
4618 TCATGA
BsrFI 2880 ACCGGC [GA]^CCGG[CT]
3615 ACCGGC
BstAPI 2155 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3907 GCAGCAGCTGC
BstXI 2020 CCAACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4197 CCAGTGGACTGG
Cfr10I 2880 ACCGGC [GA]^CCGG[CT]
3615 ACCGGC
DrdI 990 GACCTCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4229 GACTATGGAGTC
DsaI 4667 CCATGG C^C[GA][CT]GG
5297 CCATGG
EcoNI 436 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5594 CCTGAAGAAGG
EcoRI 3415 GAATTC G^AATTC
3556 GAATTC
HaeII 3183 GGCGCC [GA]GCGC^[CT]
3867 AGCGCC
Hin1I 1044 GACGTC G[GA]^CG[CT]C
3183 GGCGCC
HincII 97 GTTAAC GT[CT]^[GA]AC
4636 GTTAAC
HindII 97 GTTAAC GT[CT]^[GA]AC
4636 GTTAAC
HindIII 2014 AAGCTT A^AGCTT
3053 AAGCTT
HpaI 97 GTTAAC GTT^AAC
4636 GTTAAC
KpnI 469 GGTACC GGTAC^C
3278 GGTACC
MluNI 4206 TGGCCA TGG^CCA
4648 TGGCCA
MscI 4206 TGGCCA TGG^CCA
4648 TGGCCA
MslI 2038 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5087 CATATGTATG
NcoI 4667 CCATGG C^CATGG
5297 CCATGG
NdeI 2090 CATATG CA^TATG
5087 CATATG
PpuMI 1927 AGGTCCC [GA]G^G[AT]CC[CT]
3547 GGGACCT
PstI 1017 CTGCAG CTGCA^G
2162 CTGCAG
RcaI 2037 TCATGA T^CATGA
4618 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
AlwI AlwNI ApoI Asp700I AspEI
AspHI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI Eam1105I
EarI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI