##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AclI AflII AgeI AseI AsnI
AvrII BamHI BciVI BclI BfrI
BglII BlnI BsaBI BsiWI BspDI
BspHI BspLU11I BssSI Bst1107I BstBI
BstYI BstZ17I ClaI DraI EcoRV
EcoT22I FbaI HpaI MamI MfeI
MflI MluI MunI NdeI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
SapI SfuI SnaBI SpeI SwaI
XcmI XhoII
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 2023 GGTACC G^GTACC
AccIII 3098 TCCGGA T^CCGGA
AcsI 4190 GAATTC [GA]^AATT[CT]
AlwI 745 GGATC GGATC(4/5)
Aor51HI 2657 AGCGCT AGC^GCT
ApoI 4190 GAATTC [GA]^AATT[CT]
AscI 1386 GGCGCGCC GG^CGCGCC
Asp700I 269 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2023 GGTACC G^GTACC
AviII 3478 TGCGCA TGC^GCA
BbrPI 3909 CACGTG CAC^GTG
BbsI 2613 GTCTTC GAAGAC(2/6)
BpuAI 2613 GTCTTC GAAGAC(2/6)
BsaAI 3909 CACGTG [CT]AC^GT[GA]
BseAI 3098 TCCGGA T^CCGGA
BsmBI 1952 CGTCTC CGTCTC(1/5)
BsmI 56 GAATGC GAATGC(1/-1)
Bsp1407I 4255 TGTACA T^GTACA
BspEI 3098 TCCGGA T^CCGGA
BsrDI 5036 GCAATG GCAATG(2/0)
BsrGI 4255 TGTACA T^GTACA
Eco47III 2657 AGCGCT AGC^GCT
EcoRI 4190 GAATTC G^AATTC
FspI 3478 TGCGCA TGC^GCA
HincII 1608 GTCGAC GT[CT]^[GA]AC
HindII 1608 GTCGAC GT[CT]^[GA]AC
KpnI 2023 GGTACC GGTAC^C
MroI 3098 TCCGGA T^CCGGA
NheI 4217 GCTAGC G^CTAGC
NspI 4344 GCATGC [GA]CATG^[CT]
PaeR7I 2859 CTCGAG C^TCGAG
PmaCI 3909 CACGTG CAC^GTG
PmlI 3909 CACGTG CAC^GTG
PshAI 3223 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1608 GTCGAC G^TCGAC
ScaI 1444 AGTACT AGT^ACT
SexAI 4 ACCAGGT A^CC[AT]GGT
SfiI 1322 GGCCTTCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 3430 CGCCGGCG C[GA]^CCGG[CT]G
SphI 4344 GCATGC GCATG^C
SspBI 4255 TGTACA T^GTACA
SspI 4890 AATATT AAT^ATT
XbaI 4708 TCTAGA T^CTAGA
XhoI 2859 CTCGAG C^TCGAG
XmnI 269 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AatII 1611 GACGTC GACGT^C
3567 GACGTC
AccI 1608 GTCGAC GT^[AC][GT]AC
3576 GTCTAC
AhdI 2577 GACATCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3924 GACGCCGAGTC
Alw44I 606 GTGCAC G^TGCAC
1029 GTGCAC
ApaLI 606 GTGCAC G^TGCAC
1029 GTGCAC
AspEI 2577 GACATCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3924 GACGCCGAGTC
BlpI 1337 GCTGAGC GC^T[ACGT]AGC
1412 GCTCAGC
Bpu1102I 1337 GCTGAGC GC^T[ACGT]AGC
1412 GCTCAGC
CelII 1337 GCTGAGC GC^T[ACGT]AGC
1412 GCTCAGC
CpoI 907 CGGTCCG CG^G[AT]CCG
961 CGGACCG
DraIII 3 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4115 CACGCGGTG
DrdI 129 GACGTGTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3567 GACGTCACCGTC
Eam1105I 2577 GACATCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3924 GACGCCGAGTC
FseI 3235 GGCCGGCC GGCCGG^CC
3324 GGCCGGCC
HindIII 1649 AAGCTT A^AGCTT
2199 AAGCTT
MslI 209 CACAGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3905 CACGCACGTG
RsrII 907 CGGTCCG CG^G[AT]CCG
961 CGGACCG
SbfI 1361 CCTGCAGG CCTGCA^GG
2492 CCTGCAGG
Sse8387I 1361 CCTGCAGG CCTGCA^GG
2492 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI AocI ApaI AspHI
AspI AvaI AvaII BalI BanI
BanII BbeI BbvCI BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XmaCI XmaI