##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI AspEI AspI AviII
BalI BamHI BbrPI BcgI BclI
BfrI BglI BlpI Bpu1102I BsaAI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BsrFI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraIII DrdI Eam1105I
EarI Eco47III EcoNI EcoO65I EcoRV
FbaI FseI FspI HgaI HindIII
Ksp632I KspI MluI MluNI MroI
MscI NaeI NdeI NgoMIV NheI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SexAI SfiI SgrAI SnaBI
SwaI Tth111I XcmI
###################################
# ONE CUT ENZYMES ( 66 enzymes) #
###################################
Acc65I 798 GGTACC G^GTACC
AccII 6 CGCG CG^CG
AcyI 35 GGCGCC G[GA]^CG[CT]C
Alw44I 780 GTGCAC G^TGCAC
ApaLI 780 GTGCAC G^TGCAC
Asp700I 624 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 798 GGTACC G^GTACC
AvrII 2737 CCTAGG C^CTAGG
BbeI 35 GGCGCC GGCGC^C
BbsI 2209 GTCTTC GAAGAC(2/6)
BbvCI 467 CCTCAGC CCTCAGC(-5/-2)
BglII 905 AGATCT A^GATCT
BlnI 2737 CCTAGG C^CTAGG
BpmI 1064 CTCCAG CTGGAG(16/14)
BpuAI 2209 GTCTTC GAAGAC(2/6)
BsaBI 2783 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 35 GGCGCC G[GA]^CG[CT]C
BsaWI 910 TCCGGT [AT]^CCGG[AT]
BsiEI 2 CGGCCG CG[GA][CT]^CG
BsmI 3251 GAATGC GAATGC(1/-1)
Bsp1407I 1774 TGTACA T^GTACA
BsrBI 1433 GAGCGG CCGCTC(-3/-3)
BsrDI 1892 GCAATG GCAATG(2/0)
BsrGI 1774 TGTACA T^GTACA
BstAPI 515 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 6 CGCG CG^CG
BstXI 1105 CCAAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 262 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 2 CGGCCG [CT]^GGCC[GA]
EagI 2 CGGCCG C^GGCCG
EclXI 2 CGGCCG C^GGCCG
Eco52I 2 CGGCCG C^GGCCG
EcoO109I 262 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1744 ATGCAT ATGCA^T
FauI 929 GCGGG CCCGC(4/6)
HaeII 35 GGCGCC [GA]GCGC^[CT]
Hin1I 35 GGCGCC G[GA]^CG[CT]C
KasI 35 GGCGCC G^GCGCC
KpnI 798 GGTACC GGTAC^C
MamI 2783 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2891 CAATTG C^AATTG
MslI 1159 CACTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1473 CAGCTG C[AC]G^C[GT]G
MunI 2891 CAATTG C^AATTG
MvnI 6 CGCG CG^CG
NarI 35 GGCGCC GG^CGCC
NcoI 3025 CCATGG C^CATGG
NotI 1 GCGGCCGC GC^GGCCGC
NsiI 1744 ATGCAT ATGCA^T
PaeR7I 19 CTCGAG C^TCGAG
PpuMI 262 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 1473 CAGCTG CAG^CTG
SbfI 764 CCTGCAGG CCTGCA^GG
ScaI 2812 AGTACT AGT^ACT
SfoI 35 GGCGCC GGC^GCC
SmaI 857 CCCGGG CCC^GGG
SpeI 3364 ACTAGT A^CTAGT
SphI 472 GCATGC GCATG^C
Sse8387I 764 CCTGCAGG CCTGCA^GG
SspBI 1774 TGTACA T^GTACA
StuI 126 AGGCCT AGG^CCT
XbaI 343 TCTAGA T^CTAGA
XhoI 19 CTCGAG C^TCGAG
XmaCI 857 CCCGGG C^CCGGG
XmaI 857 CCCGGG C^CCGGG
XmnI 624 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 228 GGATC GGATC(4/5)
1033 GGATC
AocI 699 CCTTAGG CC^T[ACGT]AGG
3344 CCTAAGG
AvaII 263 GGTCC G^G[AT]CC
1177 GGACC
BanII 14 GAGCTC G[GA]GC[CT]^C
1022 GAGCTC
BciVI 614 GTATCC GTATCC(6/5)
790 GTATCC
BsaI 695 GAGACC GGTCTC(1/5)
2667 GGTCTC
BsmFI 1486 GGGAC GGGAC(10/14)
2488 GTCCC
BstBI 804 TTCGAA TT^CGAA
2775 TTCGAA
BstYI 905 AGATCT [GA]^GATC[CT]
1033 GGATCT
Bsu36I 699 CCTTAGG CC^T[ACGT]AGG
3344 CCTAAGG
Cac8I 472 GCATGC GC[ACGT]^[ACGT]GC
1504 GCAAGC
Cfr13I 263 GGTCC G^G[ACGT]CC
1177 GGACC
DsaI 1458 CCGTGG C^C[GA][CT]GG
3025 CCATGG
Eco81I 699 CCTTAGG CC^T[ACGT]AGG
3344 CCTAAGG
MflI 905 AGATCT [GA]^GATC[CT]
1033 GGATCT
NspV 804 TTCGAA TT^CGAA
2775 TTCGAA
PflMI 74 CCACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1825 CCATGCGGTGG
PleI 248 GAGTC GAGTC(4/5)
1258 GAGTC
PstI 368 CTGCAG CTGCA^G
765 CTGCAG
SacI 14 GAGCTC GAGCT^C
1022 GAGCTC
Sau96I 263 GGTCC G^G[ACGT]CC
1177 GGACC
SfuI 804 TTCGAA TT^CGAA
2775 TTCGAA
Van91I 74 CCACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1825 CCATGCGGTGG
XhoII 905 AGATCT [GA]^GATC[CT]
1033 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwNI ApoI AspHI AvaI BanI
BbvI BcnI BfaI BmyI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsoBI Bsp1286I BspLU11I BspMI
BsrI BssKI BstF5I BstNI CfoI
DdeI DpnII DraI EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I