##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AviII       
AvrII       BamHI       BbrPI       BclI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaI        BseAI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstXI       BstZ17I     CelII       
ClaI        CpoI        DrdI        Eco47III    EcoT22I     
FbaI        FseI        FspI        HindIII     KpnI        
KspI        MfeI        MluI        MroI        MunI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI         3149  GTAGAC               GT^[AC][GT]AC
AccII         219  CGCG                 CG^CG
AflIII       4079  ACATGT               A^C[GA][CT]GT
AspI         1872  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         3267  TGGCCA               TGG^CCA
BbeI         3250  GGCGCC               GGCGC^C
BcgI         2583  CGATCCTGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI         107  ACCGGA               [AT]^CCGG[AT]
BspHI        4486  TCATGA               T^CATGA
BspLU11I     4079  ACATGT               A^CATGT
BsrBI         157  GAGCGG               CCGCTC(-3/-3)
BssSI         461  CACGAG               CACGAG(-5/-1)
BstEII       1647  GGTTACC              G^GT[ACGT]ACC
BstPI        1647  GGTTACC              G^GT[ACGT]ACC
BstUI         219  CGCG                 CG^CG
CfoI         3251  GCGC                 GCG^C
DraI         4280  TTTAAA               TTT^AAA
DraIII       4009  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      1647  GGTTACC              G^GT[ACGT]ACC
HaeII        3250  GGCGCC               [GA]GCGC^[CT]
HhaI         3251  GCGC                 GCG^C
HinP1I       3251  GCGC                 G^CGC
HpaI         2827  GTTAAC               GTT^AAC
KasI         3250  GGCGCC               G^GCGCC
MaeII        1801  ACGT                 A^CGT
MluNI        3267  TGGCCA               TGG^CCA
MscI         3267  TGGCCA               TGG^CCA
MvnI          219  CGCG                 CG^CG
NaeI          135  GCCGGC               GCC^GGC
NarI         3250  GGCGCC               GG^CGCC
NdeI         2886  CATATG               CA^TATG
NgoMIV        135  GCCGGC               G^CCGGC
NheI         4171  GCTAGC               G^CTAGC
PflFI        1872  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI         4089  CTGCAG               CTGCA^G
RcaI         4486  TCATGA               T^CATGA
SacI         1918  GAGCTC               GAGCT^C
SapI         2626  GAAGAGC              GCTCTTC(1/4)
ScaI          420  AGTACT               AGT^ACT
SfoI         3250  GGCGCC               GGC^GCC
TaqI         1008  TCGA                 T^CGA
Tth111I      1872  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1008  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AcyI          138  GGCGTC               G[GA]^CG[CT]C
             3250  GGCGCC              
AhdI         2011  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4403  GACCAGAAGTC         
AlwNI         124  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1316  CAGGAGCTG           
AocI         1037  CCTCAGG              CC^T[ACGT]AGG
             2905  CCTCAGG             
Asp700I      1088  GAACTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2857  GAATCCATTC          
AspEI        2011  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4403  GACCAGAAGTC         
AvaI            8  CCCGGG               C^[CT]CG[GA]G
             3052  CCCGGG              
BbvCI         430  GCTGAGG              CCTCAGC(-5/-2)
             3202  GCTGAGG             
BciVI        3941  GTATCC               GTATCC(6/5)
             4073  GTATCC              
BsaBI        2749  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3577  GATGATGATC          
BsaHI         138  GGCGTC               G[GA]^CG[CT]C
             3250  GGCGCC              
BsiEI         160  CGGCCG               CG[GA][CT]^CG
              212  CGGCCG              
BsmI         4041  GAATGC               GAATGC(1/-1)
             2433  GCATTC              
BsoBI           8  CCCGGG               C^[CT]CG[GA]G
             3052  CCCGGG              
BspMI        2015  GCAGGT               ACCTGC(4/8)
             4509  GCAGGT              
BsrDI        2124  CATTGC               GCAATG(2/0)
             3231  CATTGC              
BsrFI         135  GCCGGC               [GA]^CCGG[CT]
             3067  GCCGGT              
Bsu36I       1037  CCTCAGG              CC^T[ACGT]AGG
             2905  CCTCAGG             
Cfr10I        135  GCCGGC               [GA]^CCGG[CT]
             3067  GCCGGT              
EagI          160  CGGCCG               C^GGCCG
              212  CGGCCG              
Eam1105I     2011  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4403  GACCAGAAGTC         
EclXI         160  CGGCCG               C^GGCCG
              212  CGGCCG              
Eco52I        160  CGGCCG               C^GGCCG
              212  CGGCCG              
Eco81I       1037  CCTCAGG              CC^T[ACGT]AGG
             2905  CCTCAGG             
EcoRI         244  GAATTC               G^AATTC
             4522  GAATTC              
HgaI          217  GACGC                GACGC(5/10)
              139  GCGTC               
Hin1I         138  GGCGTC               G[GA]^CG[CT]C
             3250  GGCGCC              
HincII       2674  GTTGAC               GT[CT]^[GA]AC
             2827  GTTAAC              
HindII       2674  GTTGAC               GT[CT]^[GA]AC
             2827  GTTAAC              
MamI         2749  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3577  GATGATGATC          
NcoI         1001  CCATGG               C^CATGG
             2461  CCATGG              
NspI         4079  ACATGT               [GA]CATG^[CT]
             4541  GCATGT              
PvuII        1856  CAGCTG               CAG^CTG
             3326  CAGCTG              
SmaI            8  CCCGGG               CCC^GGG
             3052  CCCGGG              
SmlI          424  CTCAAG               C^T[CT][GA]AG
             1672  CTCAAG              
SspI         1978  AATATT               AAT^ATT
             4250  AATATT              
XmaCI           8  CCCGGG               C^CCGGG
             3052  CCCGGG              
XmaI            8  CCCGGG               C^CCGGG
             3052  CCCGGG              
XmnI         1088  GAACTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2857  GAATCCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BglII       
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoRV       EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PpuMI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Van91I      XcmI        XhoII