##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccIII AflII AgeI Aor51HI AscI
Asp700I BamHI BbeI BciVI BclI
BfrI BglII BsaBI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspEI BsrGI
BssHII Bst1107I BstBI BstZ17I EagI
EclXI Eco47III Eco52I EcoRI FbaI
FseI HindIII HpaI KasI KspI
MamI MfeI MluI MroI MunI
NarI NheI NotI NruI NspV
PacI PinAI PmeI PvuI SacII
ScaI SexAI SfoI SfuI SmaI
SnaBI SpeI SspBI SwaI XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AclI 2501 AACGTT AA^CGTT
Alw44I 471 GTGCAC G^TGCAC
ApaI 4888 GGGCCC GGGCC^C
ApaLI 471 GTGCAC G^TGCAC
AseI 4159 ATTAAT AT^TAAT
AsnI 4159 ATTAAT AT^TAAT
AspI 1599 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4531 CCTAGG C^CTAGG
BlnI 4531 CCTAGG C^CTAGG
BsiEI 2007 CGGTCG CG[GA][CT]^CG
BspDI 1914 ATCGAT AT^CGAT
BspHI 344 TCATGA T^CATGA
ClaI 1914 ATCGAT AT^CGAT
CpoI 5142 CGGACCG CG^G[AT]CCG
EcoRV 582 GATATC GAT^ATC
HaeII 5433 GGCGCT [GA]GCGC^[CT]
NaeI 4268 GCCGGC GCC^GGC
NdeI 1693 CATATG CA^TATG
NgoMIV 4268 GCCGGC G^CCGGC
PaeR7I 1941 CTCGAG C^TCGAG
PflFI 1599 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 4159 ATTAAT AT^TAAT
Psp1406I 2501 AACGTT AA^CGTT
RcaI 344 TCATGA T^CATGA
RsrII 5142 CGGACCG CG^G[AT]CCG
SalI 2009 GTCGAC G^TCGAC
SbfI 1592 CCTGCAGG CCTGCA^GG
SfiI 524 GGCCAAGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 498 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 1592 CCTGCAGG CCTGCA^GG
Tth111I 1599 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1941 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 693 GACGTC GACGT^C
5070 GACGTC
Acc65I 373 GGTACC G^GTACC
3623 GGTACC
AccI 1281 GTCTAC GT^[AC][GT]AC
2009 GTCGAC
AlwNI 360 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
2535 CAGAGCCTG
Asp718I 373 GGTACC G^GTACC
3623 GGTACC
BbrPI 2753 CACGTG CAC^GTG
4306 CACGTG
BbvCI 4605 CCTCAGC CCTCAGC(-5/-2)
4926 CCTCAGC
BlpI 3155 GCTGAGC GC^T[ACGT]AGC
4319 GCTCAGC
Bpu1102I 3155 GCTGAGC GC^T[ACGT]AGC
4319 GCTCAGC
BsaI 1548 GAGACC GGTCTC(1/5)
2961 GAGACC
BsmI 1739 GAATGC GAATGC(1/-1)
4010 GCATTC
BspLU11I 3795 ACATGT A^CATGT
5479 ACATGT
BssSI 1908 CTCGTG CACGAG(-5/-1)
2400 CTCGTG
BstEII 2802 GGTGACC G^GT[ACGT]ACC
3260 GGTGACC
BstPI 2802 GGTGACC G^GT[ACGT]ACC
3260 GGTGACC
CelII 3155 GCTGAGC GC^T[ACGT]AGC
4319 GCTCAGC
DraI 3647 TTTAAA TTT^AAA
3707 TTTAAA
DraIII 1421 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
4303 CACCACGTG
EcoO65I 2802 GGTGACC G^GT[ACGT]ACC
3260 GGTGACC
EcoT22I 4862 ATGCAT ATGCA^T
5451 ATGCAT
KpnI 373 GGTACC GGTAC^C
3623 GGTACC
NcoI 571 CCATGG C^CATGG
4276 CCATGG
NsiI 4862 ATGCAT ATGCA^T
5451 ATGCAT
PmaCI 2753 CACGTG CAC^GTG
4306 CACGTG
PmlI 2753 CACGTG CAC^GTG
4306 CACGTG
PshAI 2834 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5154 GACAGCCGTC
SspI 337 AATATT AAT^ATT
3537 AATATT
StuI 1412 AGGCCT AGG^CCT
1805 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AocI
ApoI AspEI AspHI AvaI AvaII
AviII BalI BanI BanII BbsI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII