##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccIII AflII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AviII BbeI BcgI BfrI BglI
BlpI Bpu1102I BsaWI BseAI BsiWI
BspDI BspEI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI FauI FseI FspI
HpaI KasI MluI MroI NaeI
NarI NcoI NgoMIV NheI NotI
PacI PinAI PmeI RsrII SalI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 67 enzymes) #
###################################
Acc65I 6592 GGTACC G^GTACC
AclI 2118 AACGTT AA^CGTT
AcyI 4715 GACGCC G[GA]^CG[CT]C
AhdI 974 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 6592 GGTACC G^GTACC
AspEI 974 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1311 CTCGAG C^[CT]CG[GA]G
AvrII 3482 CCTAGG C^CTAGG
BalI 3448 TGGCCA TGG^CCA
BamHI 4542 GGATCC G^GATCC
BbrPI 1140 CACGTG CAC^GTG
BbvCI 5440 GCTGAGG CCTCAGC(-5/-2)
BciVI 6300 GTATCC GTATCC(6/5)
BcnI 21 CCGGG CC^[GC]GG
BlnI 3482 CCTAGG C^CTAGG
BsaHI 4715 GACGCC G[GA]^CG[CT]C
BsiEI 922 CGATCG CG[GA][CT]^CG
BsmBI 1282 GAGACG CGTCTC(1/5)
BsoBI 1311 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2853 TGTACA T^GTACA
BspHI 514 TCATGA T^CATGA
BsrGI 2853 TGTACA T^GTACA
BstBI 2802 TTCGAA TT^CGAA
BstEII 1083 GGTCACC G^GT[ACGT]ACC
BstPI 1083 GGTCACC G^GT[ACGT]ACC
DraIII 1524 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 72 CCGCGG C^C[GA][CT]GG
Eam1105I 974 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1083 GGTCACC G^GT[ACGT]ACC
EcoRI 3706 GAATTC G^AATTC
EcoT22I 4341 ATGCAT ATGCA^T
HaeII 3828 AGCGCC [GA]GCGC^[CT]
HapII 21 CCGG C^CGG
Hin1I 4715 GACGCC G[GA]^CG[CT]C
HpaII 21 CCGG C^CGG
KpnI 6592 GGTACC GGTAC^C
KspI 72 CCGCGG CCGC^GG
MluNI 3448 TGGCCA TGG^CCA
MscI 3448 TGGCCA TGG^CCA
MspI 21 CCGG C^CGG
NciI 21 CCGGG CC^[GC]GG
NdeI 2750 CATATG CA^TATG
NruI 919 TCGCGA TCG^CGA
NsiI 4341 ATGCAT ATGCA^T
NspV 2802 TTCGAA TT^CGAA
PaeR7I 1311 CTCGAG C^TCGAG
PflMI 2197 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1140 CACGTG CAC^GTG
PmlI 1140 CACGTG CAC^GTG
PpuMI 2922 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 2118 AACGTT AA^CGTT
PstI 3564 CTGCAG CTGCA^G
PvuI 922 CGATCG CGAT^CG
PvuII 4154 CAGCTG CAG^CTG
RcaI 514 TCATGA T^CATGA
SacI 2231 GAGCTC GAGCT^C
SacII 72 CCGCGG CCGC^GG
SapI 1233 GAAGAGC GCTCTTC(1/4)
SbfI 3563 CCTGCAGG CCTGCA^GG
SexAI 1099 ACCTGGT A^CC[AT]GGT
SfuI 2802 TTCGAA TT^CGAA
SphI 3510 GCATGC GCATG^C
Sse8387I 3563 CCTGCAGG CCTGCA^GG
SspBI 2853 TGTACA T^GTACA
Van91I 2197 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5363 TCTAGA T^CTAGA
XhoI 1311 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 1965 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
3008 CAGATTCTG
BsaAI 1140 CACGTG [CT]AC^GT[GA]
3271 TACGTG
BsmFI 1476 GGGAC GGGAC(10/14)
6504 GGGAC
BsrDI 3750 CATTGC GCAATG(2/0)
2765 GCAATG
CfoI 3813 GCGC GCG^C
3829 GCGC
DraII 2922 AGGACCT [GA]G^G[ACGT]CC[CT]
3051 AGGCCCT
EaeI 66 TGGCCG [CT]^GGCC[GA]
3448 TGGCCA
EcoO109I 2922 AGGACCT [GA]G^G[ACGT]CC[CT]
3051 AGGCCCT
HgaI 4715 GACGC GACGC(5/10)
4947 GCGTC
HhaI 3813 GCGC GCG^C
3829 GCGC
HinP1I 3813 GCGC G^CGC
3829 GCGC
HincII 4261 GTTGAC GT[CT]^[GA]AC
5027 GTCAAC
HindII 4261 GTTGAC GT[CT]^[GA]AC
5027 GTCAAC
MfeI 1704 CAATTG C^AATTG
6265 CAATTG
MunI 1704 CAATTG C^AATTG
6265 CAATTG
PshAI 642 GACATAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1478 GACAGAGGTC
ScaI 545 AGTACT AGT^ACT
5947 AGTACT
StuI 1875 AGGCCT AGG^CCT
3968 AGGCCT
XcmI 2193 CCAACCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5068 CCACATAACACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI AseI
AsnI Asp700I AspHI AspI AvaII
BanI BanII BbsI BbvI BclI
BfaI BglII BmyI BpmI BpuAI
BsaBI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmI
Bsp1286I BspLU11I BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII EcoRV EcoT14I FbaI Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PflFI PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XhoII XmnI