##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AccII AccIII AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BbeI BbvCI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiWI BsmBI
BsoBI BspEI BsrBI BssHII BstAPI
BstBI BstEII BstPI BstUI Bsu36I
CelII CfoI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I FbaI FseI FspI
HaeII HgaI HhaI HinP1I HindIII
KasI KpnI KspI MamI MluI
MroI MvnI NarI NcoI NdeI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PvuI
RsrII SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 1177 GACGTC GACGT^C
AccI 3284 GTATAC GT^[AC][GT]AC
AciI 565 GCGG CCGC(-3/-1)
AclI 4468 AACGTT AA^CGTT
AcyI 1177 GACGTC G[GA]^CG[CT]C
ApaI 568 GGGCCC GGGCC^C
Asp700I 2315 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1352 GGTCC G^G[AT]CC
BalI 4809 TGGCCA TGG^CCA
BamHI 4385 GGATCC G^GATCC
BanI 1034 GGTGCC G^G[CT][GA]CC
BbrPI 3877 CACGTG CAC^GTG
BcgI 3323 GCAACCCGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3676 GTATCC GTATCC(6/5)
BsaAI 3877 CACGTG [CT]AC^GT[GA]
BsaHI 1177 GACGTC G[GA]^CG[CT]C
BsiEI 3329 CGGTCG CG[GA][CT]^CG
BspDI 2862 ATCGAT AT^CGAT
BsrFI 2512 GCCGGC [GA]^CCGG[CT]
BssSI 1332 CTCGTG CACGAG(-5/-1)
Bst1107I 3284 GTATAC GTA^TAC
BstZ17I 3284 GTATAC GTA^TAC
Cfr10I 2512 GCCGGC [GA]^CCGG[CT]
ClaI 2862 ATCGAT AT^CGAT
DraIII 687 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 4809 TGGCCA [CT]^GGCC[GA]
EcoT14I 2239 CCAAGG C^C[AT][AT]GG
EcoT22I 1077 ATGCAT ATGCA^T
FauI 565 GCGGG CCCGC(4/6)
Hin1I 1177 GACGTC G[GA]^CG[CT]C
HpaI 1598 GTTAAC GTT^AAC
MfeI 2342 CAATTG C^AATTG
MluNI 4809 TGGCCA TGG^CCA
MscI 4809 TGGCCA TGG^CCA
MspA1I 4185 CAGCTG C[AC]G^C[GT]G
MunI 2342 CAATTG C^AATTG
NaeI 2512 GCCGGC GCC^GGC
NgoMIV 2512 GCCGGC G^CCGGC
NheI 2545 GCTAGC G^CTAGC
NsiI 1077 ATGCAT ATGCA^T
PmaCI 3877 CACGTG CAC^GTG
PmlI 3877 CACGTG CAC^GTG
PshAI 4840 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4468 AACGTT AA^CGTT
PvuII 4185 CAGCTG CAG^CTG
SacI 4270 GAGCTC GAGCT^C
SexAI 4996 ACCAGGT A^CC[AT]GGT
SphI 4752 GCATGC GCATG^C
StuI 2477 AGGCCT AGG^CCT
StyI 2239 CCAAGG C^C[AT][AT]GG
SwaI 1377 ATTTAAAT ATTT^AAAT
XbaI 3229 TCTAGA T^CTAGA
XmnI 2315 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 2175 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
4182 CAGCAGCTG
AseI 3994 ATTAAT AT^TAAT
4696 ATTAAT
AsnI 3994 ATTAAT AT^TAAT
4696 ATTAAT
AspHI 1273 GTGCTC G[AT]GC[AT]^C
4270 GAGCTC
BcnI 2413 CCGGG CC^[GC]GG
3327 CCCGG
BglII 253 AGATCT A^GATCT
2944 AGATCT
BsaI 2603 GAGACC GGTCTC(1/5)
4022 GAGACC
BsiHKAI 1273 GTGCTC G[AT]GC[AT]^C
4270 GAGCTC
BspHI 3109 TCATGA T^CATGA
4957 TCATGA
BspLU11I 2378 ACATGT A^CATGT
5640 ACATGT
BsrDI 5414 CATTGC GCAATG(2/0)
3894 GCAATG
DraII 567 GGGGCCC [GA]G^G[ACGT]CC[CT]
6435 AGGGCCT
DsaI 559 CCGTGG C^C[GA][CT]GG
3241 CCACGG
EcoO109I 567 GGGGCCC [GA]G^G[ACGT]CC[CT]
6435 AGGGCCT
EcoRI 1720 GAATTC G^AATTC
1819 GAATTC
NciI 2413 CCGGG CC^[GC]GG
3327 CCCGG
PshBI 3994 ATTAAT AT^TAAT
4696 ATTAAT
RcaI 3109 TCATGA T^CATGA
4957 TCATGA
SpeI 4430 ACTAGT A^CTAGT
5544 ACTAGT
XcmI 3070 CCAAGCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4360 CCATCCGAATACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII EcoRV Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspBI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII