##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AatII AccII AccIII AscI BsaBI
BseAI BsiWI BspDI BspEI BssHII
BssSI BstBI BstUI ClaI CpoI
DraIII DrdI FseI KspI MamI
MluI MroI MvnI NotI NruI
NspV PacI PaeR7I PmeI PshAI
PvuI RsrII SacI SacII SalI
SapI SbfI SfuI SgrAI SphI
Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 1820 GGTACC G^GTACC
AclI 1313 AACGTT AA^CGTT
AhdI 516 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 5429 CCTAAGG CC^T[ACGT]AGG
Aor51HI 285 AGCGCT AGC^GCT
AseI 2951 ATTAAT AT^TAAT
AsnI 2951 ATTAAT AT^TAAT
Asp700I 1384 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1820 GGTACC G^GTACC
AspEI 516 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2572 TGCGCA TGC^GCA
BamHI 1283 GGATCC G^GATCC
BcgI 3575 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4642 TGATCA T^GATCA
BglII 3113 AGATCT A^GATCT
BlpI 6281 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 6281 GCTTAGC GC^T[ACGT]AGC
BsiEI 192 CGGCCG CG[GA][CT]^CG
BsmI 2999 GAATGC GAATGC(1/-1)
BspLU11I 6485 ACATGT A^CATGT
BsrBI 115 GAGCGG CCGCTC(-3/-3)
Bst1107I 671 GTATAC GTA^TAC
BstEII 2507 GGTCACC G^GT[ACGT]ACC
BstPI 2507 GGTCACC G^GT[ACGT]ACC
BstZ17I 671 GTATAC GTA^TAC
Bsu36I 5429 CCTAAGG CC^T[ACGT]AGG
CelII 6281 GCTTAGC GC^T[ACGT]AGC
EagI 192 CGGCCG C^GGCCG
Eam1105I 516 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 192 CGGCCG C^GGCCG
Eco47III 285 AGCGCT AGC^GCT
Eco52I 192 CGGCCG C^GGCCG
Eco81I 5429 CCTAAGG CC^T[ACGT]AGG
EcoO65I 2507 GGTCACC G^GT[ACGT]ACC
EcoRV 442 GATATC GAT^ATC
FbaI 4642 TGATCA T^GATCA
FspI 2572 TGCGCA TGC^GCA
HpaI 4621 GTTAAC GTT^AAC
KpnI 1820 GGTACC GGTAC^C
MfeI 3035 CAATTG C^AATTG
MunI 3035 CAATTG C^AATTG
NaeI 312 GCCGGC GCC^GGC
NdeI 4804 CATATG CA^TATG
NgoMIV 312 GCCGGC G^CCGGC
NheI 5379 GCTAGC G^CTAGC
PflMI 5272 CCAATATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2951 ATTAAT AT^TAAT
Psp1406I 1313 AACGTT AA^CGTT
SfiI 1245 GGCCCCACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 55 CCCGGG CCC^GGG
SnaBI 3791 TACGTA TAC^GTA
StuI 2959 AGGCCT AGG^CCT
Van91I 5272 CCAATATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5261 TCTAGA T^CTAGA
XcmI 4803 CCATATGAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 55 CCCGGG C^CCGGG
XmaI 55 CCCGGG C^CCGGG
XmnI 1384 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AflII 2411 CTTAAG C^TTAAG
5374 CTTAAG
AflIII 2608 ACACGT A^C[GA][CT]GT
6485 ACATGT
AgeI 12 ACCGGT A^CCGGT
4617 ACCGGT
Alw44I 2725 GTGCAC G^TGCAC
3783 GTGCAC
ApaLI 2725 GTGCAC G^TGCAC
3783 GTGCAC
AspI 5950 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6804 GACTCTGTC
AvaI 55 CCCGGG C^[CT]CG[GA]G
1287 CCCGAG
AvrII 2295 CCTAGG C^CTAGG
4466 CCTAGG
BbeI 1236 GGCGCC GGCGC^C
1626 GGCGCC
BbrPI 1974 CACGTG CAC^GTG
6409 CACGTG
BciVI 2273 GGATAC GTATCC(6/5)
3317 GTATCC
BfrI 2411 CTTAAG C^TTAAG
5374 CTTAAG
BglI 1246 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6624 GCCAACATGGC
BlnI 2295 CCTAGG C^CTAGG
4466 CCTAGG
BsoBI 55 CCCGGG C^[CT]CG[GA]G
1287 CCCGAG
DsaI 5542 CCATGG C^C[GA][CT]GG
6530 CCATGG
EcoRI 911 GAATTC G^AATTC
3462 GAATTC
EcoT22I 976 ATGCAT ATGCA^T
4777 ATGCAT
HgaI 1540 GACGC GACGC(5/10)
5312 GACGC
HincII 4061 GTCAAC GT[CT]^[GA]AC
4621 GTTAAC
HindII 4061 GTCAAC GT[CT]^[GA]AC
4621 GTTAAC
KasI 1236 GGCGCC G^GCGCC
1626 GGCGCC
NarI 1236 GGCGCC GG^CGCC
1626 GGCGCC
NcoI 5542 CCATGG C^CATGG
6530 CCATGG
NsiI 976 ATGCAT ATGCA^T
4777 ATGCAT
PflFI 5950 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6804 GACTCTGTC
PinAI 12 ACCGGT A^CCGGT
4617 ACCGGT
PmaCI 1974 CACGTG CAC^GTG
6409 CACGTG
PmlI 1974 CACGTG CAC^GTG
6409 CACGTG
PpuMI 1783 GGGACCT [GA]G^G[AT]CC[CT]
4364 AGGACCT
PvuII 1794 CAGCTG CAG^CTG
3767 CAGCTG
SexAI 1524 ACCAGGT A^CC[AT]GGT
3164 ACCAGGT
SfoI 1236 GGCGCC GGC^GCC
1626 GGCGCC
SpeI 4672 ACTAGT A^CTAGT
5458 ACTAGT
SspI 2985 AATATT AAT^ATT
3814 AATATT
Tth111I 5950 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6804 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I Bsp1407I BspHI BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII