##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI Aor51HI ApaI AscI AviII
BbeI BbrPI BcgI BglI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I CelII CfoI ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII KasI KspI MamI
MluI MroI MspA1I MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI PacI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 2452 GGTACC G^GTACC
AccI 2324 GTAGAC GT^[AC][GT]AC
AflIII 4296 ACATGT A^C[GA][CT]GT
Alw44I 3180 GTGCAC G^TGCAC
AocI 3938 CCTCAGG CC^T[ACGT]AGG
ApaLI 3180 GTGCAC G^TGCAC
AseI 2602 ATTAAT AT^TAAT
AsnI 2602 ATTAAT AT^TAAT
Asp718I 2452 GGTACC G^GTACC
AspI 811 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 3697 CCTCAGC CCTCAGC(-5/-2)
BciVI 1308 GGATAC GTATCC(6/5)
BclI 2135 TGATCA T^GATCA
BcnI 1866 CCGGG CC^[GC]GG
BsaI 3192 GGTCTC GGTCTC(1/5)
BsgI 2891 CTGCAC GTGCAG(16/14)
BsmBI 254 CGTCTC CGTCTC(1/5)
BsmI 2373 GAATGC GAATGC(1/-1)
BspHI 771 TCATGA T^CATGA
BspLU11I 4296 ACATGT A^CATGT
BsrBI 49 GAGCGG CCGCTC(-3/-3)
BsrFI 652 GCCGGT [GA]^CCGG[CT]
BstBI 4515 TTCGAA TT^CGAA
Bsu36I 3938 CCTCAGG CC^T[ACGT]AGG
Cfr10I 652 GCCGGT [GA]^CCGG[CT]
DraI 1734 TTTAAA TTT^AAA
DraIII 1695 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3938 CCTCAGG CC^T[ACGT]AGG
EcoNI 3675 CCTGAAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2780 ATGCAT ATGCA^T
FauI 1335 GCGGG CCCGC(4/6)
FbaI 2135 TGATCA T^GATCA
HpaI 3523 GTTAAC GTT^AAC
KpnI 2452 GGTACC GGTAC^C
MfeI 562 CAATTG C^AATTG
MslI 1116 CATAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 562 CAATTG C^AATTG
NciI 1866 CCGGG CC^[GC]GG
NsiI 2780 ATGCAT ATGCA^T
NspI 4296 ACATGT [GA]CATG^[CT]
NspV 4515 TTCGAA TT^CGAA
PaeR7I 149 CTCGAG C^TCGAG
PflFI 811 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3364 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2602 ATTAAT AT^TAAT
RcaI 771 TCATGA T^CATGA
SacI 4356 GAGCTC GAGCT^C
SfuI 4515 TTCGAA TT^CGAA
StuI 1705 AGGCCT AGG^CCT
Tth111I 811 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3364 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3620 TCTAGA T^CTAGA
XcmI 3465 CCAACTTTGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 149 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaI 149 CTCGAG C^[CT]CG[GA]G
1920 CTCGGG
BalI 725 TGGCCA TGG^CCA
2808 TGGCCA
BsoBI 149 CTCGAG C^[CT]CG[GA]G
1920 CTCGGG
BspMI 2889 ACCTGC ACCTGC(4/8)
903 GCAGGT
BstXI 1298 CCAGCTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2853 CCAGCCTCCTGG
EaeI 725 TGGCCA [CT]^GGCC[GA]
2808 TGGCCA
EarI 858 CTCTTC CTCTTC(1/4)
2403 GAAGAG
HincII 946 GTTGAC GT[CT]^[GA]AC
3523 GTTAAC
HindII 946 GTTGAC GT[CT]^[GA]AC
3523 GTTAAC
Ksp632I 858 CTCTTC CTCTTC(1/4)
2403 GAAGAG
MluNI 725 TGGCCA TGG^CCA
2808 TGGCCA
MscI 725 TGGCCA TGG^CCA
2808 TGGCCA
PpuMI 579 AGGTCCC [GA]G^G[AT]CC[CT]
2172 GGGTCCC
ScaI 2120 AGTACT AGT^ACT
3804 AGTACT
SspI 232 AATATT AAT^ATT
4290 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AflII AhdI
AluI AlwI AlwNI ApoI Asp700I
AspEI AspHI AvaII AvrII BamHI
BanI BanII BbsI BbvI BfaI
BfrI BglII BlnI BmyI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI Eam1105I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI