##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AccIII AclI AgeI AscI
AseI AsnI AviII BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DrdI EcoRV
FseI FspI HpaI KspI MfeI
MluI MroI MunI NheI NotI
NruI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI ScaI SfuI SgrAI SnaBI
SphI SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 7555 GGTACC G^GTACC
AccI 4936 GTCTAC GT^[AC][GT]AC
AflII 2525 CTTAAG C^TTAAG
Aor51HI 863 AGCGCT AGC^GCT
Asp718I 7555 GGTACC G^GTACC
BbrPI 4464 CACGTG CAC^GTG
BcgI 2951 CGAGGGTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 453 GGATAC GTATCC(6/5)
BfrI 2525 CTTAAG C^TTAAG
BsaAI 4464 CACGTG [CT]AC^GT[GA]
BsaBI 6874 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 7547 CGGCCG CG[GA][CT]^CG
BspHI 4659 TCATGA T^CATGA
BsrBI 4826 GAGCGG CCGCTC(-3/-3)
DraI 2626 TTTAAA TTT^AAA
EagI 7547 CGGCCG C^GGCCG
EclXI 7547 CGGCCG C^GGCCG
Eco47III 863 AGCGCT AGC^GCT
Eco52I 7547 CGGCCG C^GGCCG
HincII 537 GTTGAC GT[CT]^[GA]AC
HindII 537 GTTGAC GT[CT]^[GA]AC
KpnI 7555 GGTACC GGTAC^C
MamI 6874 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 6947 CTCGAG C^TCGAG
PmaCI 4464 CACGTG CAC^GTG
PmlI 4464 CACGTG CAC^GTG
RcaI 4659 TCATGA T^CATGA
SapI 1022 GCTCTTC GCTCTTC(1/4)
SbfI 1456 CCTGCAGG CCTGCA^GG
SfiI 1438 GGCCAGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 1740 ACTAGT A^CTAGT
Sse8387I 1456 CCTGCAGG CCTGCA^GG
XbaI 5096 TCTAGA T^CTAGA
XhoI 6947 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 1196 CGCG CG^CG
2322 CGCG
AocI 1483 CCTGAGG CC^T[ACGT]AGG
7702 CCTGAGG
BamHI 1721 GGATCC G^GATCC
5774 GGATCC
BbsI 2837 GTCTTC GAAGAC(2/6)
5685 GTCTTC
BclI 5819 TGATCA T^GATCA
6009 TGATCA
BglII 5077 AGATCT A^GATCT
6879 AGATCT
BpuAI 2837 GTCTTC GAAGAC(2/6)
5685 GTCTTC
BsmI 4700 GCATTC GAATGC(1/-1)
5400 GCATTC
BspLU11I 2442 ACATGT A^CATGT
4493 ACATGT
BstAPI 2283 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4147 GCAGGACCTGC
BstEII 1261 GGTGACC G^GT[ACGT]ACC
4264 GGTGACC
BstPI 1261 GGTGACC G^GT[ACGT]ACC
4264 GGTGACC
BstUI 1196 CGCG CG^CG
2322 CGCG
Bsu36I 1483 CCTGAGG CC^T[ACGT]AGG
7702 CCTGAGG
DraIII 1086 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
4721 CACTCTGTG
Eco81I 1483 CCTGAGG CC^T[ACGT]AGG
7702 CCTGAGG
EcoO65I 1261 GGTGACC G^GT[ACGT]ACC
4264 GGTGACC
EcoRI 2726 GAATTC G^AATTC
5185 GAATTC
FbaI 5819 TGATCA T^GATCA
6009 TGATCA
HgaI 1747 GCGTC GACGC(5/10)
3811 GCGTC
HindIII 6200 AAGCTT A^AGCTT
7055 AAGCTT
MaeII 3950 ACGT A^CGT
4465 ACGT
MvnI 1196 CGCG CG^CG
2322 CGCG
NaeI 2940 GCCGGC GCC^GGC
7545 GCCGGC
NdeI 806 CATATG CA^TATG
3876 CATATG
NgoMIV 2940 GCCGGC G^CCGGC
7545 GCCGGC
PshAI 1264 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6945 GACTCGAGTC
SexAI 1319 ACCTGGT A^CC[AT]GGT
3708 ACCTGGT
SmaI 4205 CCCGGG CCC^GGG
7481 CCCGGG
TaqI 2732 TCGA T^CGA
6948 TCGA
TthHB8I 2732 TCGA T^CGA
6948 TCGA
XmaCI 4205 CCCGGG C^CCGGG
7481 CCCGGG
XmaI 4205 CCCGGG C^CCGGG
7481 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AhdI AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI Asp700I AspEI
AspHI AspI AvaI AvaII AvrII
BalI BanI BanII BbeI BbvCI
BbvI BcnI BfaI BglI BlnI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I Van91I XcmI
XhoII XmnI