##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccIII AclI AcyI AflIII
AgeI Alw44I ApaLI AscI AspHI
AspI AvrII BbeI BbrPI BcgI
BciVI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BssHII BssSI Bst1107I
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI DsaI EagI
EclXI Eco52I EcoT22I FseI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SgrAI
SnaBI SphI Sse8387I SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1329 GGTACC G^GTACC
AflII 447 CTTAAG C^TTAAG
Aor51HI 808 AGCGCT AGC^GCT
ApaI 743 GGGCCC GGGCC^C
AseI 2200 ATTAAT AT^TAAT
AsnI 2200 ATTAAT AT^TAAT
Asp700I 2385 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1329 GGTACC G^GTACC
AviII 10 TGCGCA TGC^GCA
BamHI 2502 GGATCC G^GATCC
BclI 1805 TGATCA T^GATCA
BfrI 447 CTTAAG C^TTAAG
BsaAI 2179 TACGTG [CT]AC^GT[GA]
BsgI 492 CTGCAC GTGCAG(16/14)
Bsp1407I 1898 TGTACA T^GTACA
BspHI 2432 TCATGA T^CATGA
BspMI 2009 ACCTGC ACCTGC(4/8)
BsrBI 1325 GAGCGG CCGCTC(-3/-3)
BsrFI 1886 GCCGGC [GA]^CCGG[CT]
BsrGI 1898 TGTACA T^GTACA
BstBI 2094 TTCGAA TT^CGAA
BstEII 1074 GGTGACC G^GT[ACGT]ACC
BstPI 1074 GGTGACC G^GT[ACGT]ACC
BstYI 2502 GGATCC [GA]^GATC[CT]
Cfr10I 1886 GCCGGC [GA]^CCGG[CT]
Eco47III 808 AGCGCT AGC^GCT
EcoO65I 1074 GGTGACC G^GT[ACGT]ACC
EcoRV 1921 GATATC GAT^ATC
FbaI 1805 TGATCA T^GATCA
FspI 10 TGCGCA TGC^GCA
KpnI 1329 GGTACC GGTAC^C
MflI 2502 GGATCC [GA]^GATC[CT]
MslI 1068 CACTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1886 GCCGGC GCC^GGC
NgoMIV 1886 GCCGGC G^CCGGC
NspV 2094 TTCGAA TT^CGAA
PflMI 463 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2200 ATTAAT AT^TAAT
RcaI 2432 TCATGA T^CATGA
SexAI 1357 ACCAGGT A^CC[AT]GGT
SfuI 2094 TTCGAA TT^CGAA
SmlI 447 CTTAAG C^T[CT][GA]AG
SpeI 2379 ACTAGT A^CTAGT
SspBI 1898 TGTACA T^GTACA
StuI 1782 AGGCCT AGG^CCT
Van91I 463 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 2502 GGATCC [GA]^GATC[CT]
XmnI 2385 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 568 GTCTAC GT^[AC][GT]AC
1408 GTAGAC
AccII 505 CGCG CG^CG
1654 CGCG
AocI 242 CCTTAGG CC^T[ACGT]AGG
871 CCTAAGG
BalI 611 TGGCCA TGG^CCA
1847 TGGCCA
BbsI 1592 GAAGAC GAAGAC(2/6)
306 GTCTTC
BpuAI 1592 GAAGAC GAAGAC(2/6)
306 GTCTTC
BsaI 590 GAGACC GGTCTC(1/5)
1567 GAGACC
BstUI 505 CGCG CG^CG
1654 CGCG
Bsu36I 242 CCTTAGG CC^T[ACGT]AGG
871 CCTAAGG
EaeI 611 TGGCCA [CT]^GGCC[GA]
1847 TGGCCA
Eco81I 242 CCTTAGG CC^T[ACGT]AGG
871 CCTAAGG
EcoNI 219 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1491 CCTGGCAGAGG
EcoRI 229 GAATTC G^AATTC
2266 GAATTC
HaeII 808 AGCGCT [GA]GCGC^[CT]
1613 AGCGCC
MaeII 133 ACGT A^CGT
2180 ACGT
MluNI 611 TGGCCA TGG^CCA
1847 TGGCCA
MscI 611 TGGCCA TGG^CCA
1847 TGGCCA
MvnI 505 CGCG CG^CG
1654 CGCG
SmaI 925 CCCGGG CCC^GGG
1123 CCCGGG
TaqI 686 TCGA T^CGA
2095 TCGA
TthHB8I 686 TCGA T^CGA
2095 TCGA
XcmI 599 CCAAGAAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
843 CCAAGAGGCAGATGG
XmaCI 925 CCCGGG C^CCGGG
1123 CCCGGG
XmaI 925 CCCGGG C^CCGGG
1123 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
AlwI AlwNI ApoI AspEI AvaI
AvaII BanI BanII BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
Eam1105I EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI