##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AocI ApaI AscI AseI
AsnI AspI AvaI AviII BamHI
BbeI BbrPI BbvCI BclI BcnI
BfrI BglI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Bsu36I
CelII ClaI CpoI EagI EclXI
Eco52I Eco81I EcoRI FbaI FseI
FspI Hin1I KasI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 27 GGTACC G^GTACC
AflIII 4226 ACATGT A^C[GA][CT]GT
AgeI 2508 ACCGGT A^CCGGT
Alw44I 5014 GTGCAC G^TGCAC
Aor51HI 5310 AGCGCT AGC^GCT
ApaLI 5014 GTGCAC G^TGCAC
Asp718I 27 GGTACC G^GTACC
BalI 965 TGGCCA TGG^CCA
BbsI 4552 GAAGAC GAAGAC(2/6)
BcgI 4813 GCAAAAATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2382 GTATCC GTATCC(6/5)
BglII 4706 AGATCT A^GATCT
BpuAI 4552 GAAGAC GAAGAC(2/6)
BsaWI 2508 ACCGGT [AT]^CCGG[AT]
BsgI 2665 CTGCAC GTGCAG(16/14)
BsmBI 1031 CGTCTC CGTCTC(1/5)
Bsp1407I 5414 TGTACA T^GTACA
BspLU11I 4226 ACATGT A^CATGT
BsrBI 2545 GAGCGG CCGCTC(-3/-3)
BsrGI 5414 TGTACA T^GTACA
CfoI 5311 GCGC GCG^C
DrdI 2728 GACTTTTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5310 AGCGCT AGC^GCT
EcoNI 3372 CCTCCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 5310 AGCGCT [GA]GCGC^[CT]
HgaI 2735 GCGTC GACGC(5/10)
HhaI 5311 GCGC GCG^C
HinP1I 5311 GCGC G^CGC
KpnI 27 GGTACC GGTAC^C
MluNI 965 TGGCCA TGG^CCA
MscI 965 TGGCCA TGG^CCA
PinAI 2508 ACCGGT A^CCGGT
ScaI 4612 AGTACT AGT^ACT
SphI 4996 GCATGC GCATG^C
SspBI 5414 TGTACA T^GTACA
StuI 2655 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AhdI 763 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1392 GACAAAAAGTC
AspEI 763 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1392 GACAAAAAGTC
BspHI 441 TCATGA T^CATGA
3813 TCATGA
BsrDI 335 CATTGC GCAATG(2/0)
2821 CATTGC
BsrFI 1073 ACCGGC [GA]^CCGG[CT]
2508 ACCGGT
BstAPI 3591 GCATTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4783 GCATTAGATGC
BstEII 1549 GGTGACC G^GT[ACGT]ACC
2041 GGTTACC
BstPI 1549 GGTGACC G^GT[ACGT]ACC
2041 GGTTACC
Cfr10I 1073 ACCGGC [GA]^CCGG[CT]
2508 ACCGGT
DraIII 175 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
969 CACAAGGTG
Eam1105I 763 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1392 GACAAAAAGTC
EcoO65I 1549 GGTGACC G^GT[ACGT]ACC
2041 GGTTACC
EcoRV 4146 GATATC GAT^ATC
4319 GATATC
EcoT22I 3266 ATGCAT ATGCA^T
4994 ATGCAT
HapII 1074 CCGG C^CGG
2509 CCGG
HindIII 2207 AAGCTT A^AGCTT
2924 AAGCTT
HpaII 1074 CCGG C^CGG
2509 CCGG
MspI 1074 CCGG C^CGG
2509 CCGG
NdeI 1124 CATATG CA^TATG
2196 CATATG
NsiI 3266 ATGCAT ATGCA^T
4994 ATGCAT
PvuII 1079 CAGCTG CAG^CTG
5140 CAGCTG
RcaI 441 TCATGA T^CATGA
3813 TCATGA
SexAI 23 ACCTGGT A^CC[AT]GGT
4623 ACCTGGT
SpeI 1701 ACTAGT A^CTAGT
5508 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII AvrII BanI BanII BbvI
BfaI BlnI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HincII HindII HinfI
HpaI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XcmI
XhoII XmnI