##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AhdI Alw44I Aor51HI
ApaLI AscI AspEI AspI AviII
AvrII BbeI BbrPI BcgI BlnI
BsaAI BseAI BsiEI BsiWI BspDI
BspEI BsrBI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FseI FspI HaeII KasI KspI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PpuMI PvuI RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 3352 CGCG CG^CG
AcyI 2358 GACGCC G[GA]^CG[CT]C
AgeI 1492 ACCGGT A^CCGGT
AocI 3747 CCTAAGG CC^T[ACGT]AGG
ApaI 5231 GGGCCC GGGCC^C
AvaI 3857 CTCGGG C^[CT]CG[GA]G
BamHI 6282 GGATCC G^GATCC
BbvCI 3729 GCTGAGG CCTCAGC(-5/-2)
BciVI 394 GGATAC GTATCC(6/5)
BcnI 3822 CCGGG CC^[GC]GG
BglI 6788 GCCCCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 446 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 446 GCTAAGC GC^T[ACGT]AGC
BsaHI 2358 GACGCC G[GA]^CG[CT]C
BsaWI 1492 ACCGGT [AT]^CCGG[AT]
BsoBI 3857 CTCGGG C^[CT]CG[GA]G
BspHI 6180 TCATGA T^CATGA
BspMI 5215 ACCTGC ACCTGC(4/8)
BsrFI 1492 ACCGGT [GA]^CCGG[CT]
BstEII 1495 GGTCACC G^GT[ACGT]ACC
BstPI 1495 GGTCACC G^GT[ACGT]ACC
BstUI 3352 CGCG CG^CG
BstXI 3714 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3747 CCTAAGG CC^T[ACGT]AGG
CelII 446 GCTAAGC GC^T[ACGT]AGC
CfoI 3353 GCGC GCG^C
Cfr10I 1492 ACCGGT [GA]^CCGG[CT]
DraIII 1980 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 6170 GACTCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3747 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1495 GGTCACC G^GT[ACGT]ACC
EcoRI 6400 GAATTC G^AATTC
FauI 3834 GCGGG CCCGC(4/6)
HgaI 2358 GACGC GACGC(5/10)
HhaI 3353 GCGC GCG^C
Hin1I 2358 GACGCC G[GA]^CG[CT]C
HinP1I 3353 GCGC G^CGC
HincII 6062 GTTAAC GT[CT]^[GA]AC
HindII 6062 GTTAAC GT[CT]^[GA]AC
HpaI 6062 GTTAAC GTT^AAC
MvnI 3352 CGCG CG^CG
NciI 3822 CCGGG CC^[GC]GG
NcoI 6417 CCATGG C^CATGG
NheI 4658 GCTAGC G^CTAGC
PinAI 1492 ACCGGT A^CCGGT
PshAI 5826 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 6180 TCATGA T^CATGA
SacI 944 GAGCTC GAGCT^C
SexAI 3980 ACCAGGT A^CC[AT]GGT
SphI 1187 GCATGC GCATG^C
StuI 2804 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 217 GTCTAC GT^[AC][GT]AC
5172 GTCTAC
AciI 348 CCGC CCGC(-3/-1)
3834 GCGG
AclI 2435 AACGTT AA^CGTT
6495 AACGTT
AlwNI 962 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1728 CAGCTTCTG
Asp700I 590 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5397 GAAGAAATTC
AvaII 1255 GGACC G^G[AT]CC
6210 GGTCC
BclI 4506 TGATCA T^GATCA
6503 TGATCA
BglII 1101 AGATCT A^GATCT
1908 AGATCT
BsaI 322 GAGACC GGTCTC(1/5)
3764 GAGACC
BsgI 5758 CTGCAC GTGCAG(16/14)
3429 GTGCAG
BsmBI 3966 CGTCTC CGTCTC(1/5)
2356 GAGACG
BstAPI 1191 GCAAACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3897 GCAGAGGTTGC
DraII 5230 GGGGCCC [GA]G^G[ACGT]CC[CT]
5231 GGGCCCC
DsaI 1464 CCACGG C^C[GA][CT]GG
6417 CCATGG
EcoO109I 5230 GGGGCCC [GA]G^G[ACGT]CC[CT]
5231 GGGCCCC
FbaI 4506 TGATCA T^GATCA
6503 TGATCA
HapII 1493 CCGG C^CGG
3822 CCGG
HindIII 5886 AAGCTT A^AGCTT
5990 AAGCTT
HpaII 1493 CCGG C^CGG
3822 CCGG
MspI 1493 CCGG C^CGG
3822 CCGG
NdeI 4649 CATATG CA^TATG
5878 CATATG
Psp1406I 2435 AACGTT AA^CGTT
6495 AACGTT
SpeI 871 ACTAGT A^CTAGT
5006 ACTAGT
XbaI 3287 TCTAGA T^CTAGA
6443 TCTAGA
XmnI 590 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5397 GAAGAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
Acc65I AcsI AfaI AflII AflIII
AluI AlwI ApoI AseI AsnI
Asp718I AspHI BalI BanI BanII
BbsI BbvI BfaI BfrI BmyI
BpmI BpuAI BsaBI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspLU11I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EaeI EarI EcoNI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HinfI HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII