##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AclI AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI AspEI AvrII BbeI BcgI
BfrI BlnI BsaBI BsiEI BsiWI
Bsp1407I BspDI BspLU11I BsrGI BssHII
Bst1107I BstAPI BstEII BstPI BstZ17I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoT22I FseI
HincII HindII HpaI KasI KspI
MamI MluI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI ScaI SfoI SgrAI SnaBI
SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 2215 GACGTC GACGT^C
AccI 2319 GTCTAC GT^[AC][GT]AC
AccIII 5499 TCCGGA T^CCGGA
Aor51HI 3240 AGCGCT AGC^GCT
Asp700I 3215 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 534 TGCGCA TGC^GCA
BamHI 5597 GGATCC G^GATCC
BbrPI 7396 CACGTG CAC^GTG
BclI 2602 TGATCA T^GATCA
BsaAI 7396 CACGTG [CT]AC^GT[GA]
BseAI 5499 TCCGGA T^CCGGA
BspEI 5499 TCCGGA T^CCGGA
BsrFI 5568 ACCGGC [GA]^CCGG[CT]
BstBI 1749 TTCGAA TT^CGAA
Cfr10I 5568 ACCGGC [GA]^CCGG[CT]
DraI 6919 TTTAAA TTT^AAA
DraIII 6083 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 7121 CCACGG C^C[GA][CT]GG
Eco47III 3240 AGCGCT AGC^GCT
EcoRV 7653 GATATC GAT^ATC
FbaI 2602 TGATCA T^GATCA
FspI 534 TGCGCA TGC^GCA
MfeI 6142 CAATTG C^AATTG
MroI 5499 TCCGGA T^CCGGA
MunI 6142 CAATTG C^AATTG
NspV 1749 TTCGAA TT^CGAA
PmaCI 7396 CACGTG CAC^GTG
PmlI 7396 CACGTG CAC^GTG
SbfI 4879 CCTGCAGG CCTGCA^GG
SfiI 5194 GGCCAACCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1749 TTCGAA TT^CGAA
SpeI 3058 ACTAGT A^CTAGT
Sse8387I 4879 CCTGCAGG CCTGCA^GG
SspI 2508 AATATT AAT^ATT
XbaI 1957 TCTAGA T^CTAGA
XmnI 3215 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 316 GGTACC G^GTACC
5355 GGTACC
AccII 834 CGCG CG^CG
2033 CGCG
Asp718I 316 GGTACC G^GTACC
5355 GGTACC
AspI 932 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5003 GACATAGTC
BalI 6490 TGGCCA TGG^CCA
7736 TGGCCA
BglI 1721 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5195 GCCAACCAGGC
BsaWI 4269 TCCGGT [AT]^CCGG[AT]
5499 TCCGGA
BstUI 834 CGCG CG^CG
2033 CGCG
EcoRI 1135 GAATTC G^AATTC
6310 GAATTC
KpnI 316 GGTACC GGTAC^C
5355 GGTACC
MluNI 6490 TGGCCA TGG^CCA
7736 TGGCCA
MscI 6490 TGGCCA TGG^CCA
7736 TGGCCA
MvnI 834 CGCG CG^CG
2033 CGCG
NheI 59 GCTAGC G^CTAGC
814 GCTAGC
PflFI 932 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5003 GACATAGTC
SmaI 223 CCCGGG CCC^GGG
4578 CCCGGG
SphI 558 GCATGC GCATG^C
4645 GCATGC
Tth111I 932 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5003 GACATAGTC
XmaCI 223 CCCGGG C^CCGGG
4578 CCCGGG
XmaI 223 CCCGGG C^CCGGG
4578 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrI
BssKI BssSI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII