##################################
# NO CUT ENZYMES ( 35 enzymes) #
##################################
AgeI AscI AviII BbrPI BsiEI
BsiWI BssHII Bst1107I BstBI BstZ17I
CpoI DrdI EagI EclXI Eco52I
FseI FspI MluI NheI NotI
NruI NspV PinAI PmaCI PmlI
PshAI PvuI RsrII SalI SbfI
SfiI SfuI SgrAI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 6231 GACGTC GACGT^C
AccI 5031 GTCTAC GT^[AC][GT]AC
AflII 8490 CTTAAG C^TTAAG
AhdI 4953 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2010 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2518 AGCGCT AGC^GCT
AspEI 4953 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2810 CCTAGG C^CTAGG
BfrI 8490 CTTAAG C^TTAAG
BlnI 2810 CCTAGG C^CTAGG
BlpI 8385 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 8385 GCTGAGC GC^T[ACGT]AGC
BspDI 4614 ATCGAT AT^CGAT
BstEII 9431 GGTGACC G^GT[ACGT]ACC
BstPI 9431 GGTGACC G^GT[ACGT]ACC
Bsu36I 2010 CCTGAGG CC^T[ACGT]AGG
CelII 8385 GCTGAGC GC^T[ACGT]AGC
ClaI 4614 ATCGAT AT^CGAT
Eam1105I 4953 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2518 AGCGCT AGC^GCT
Eco81I 2010 CCTGAGG CC^T[ACGT]AGG
EcoO65I 9431 GGTGACC G^GT[ACGT]ACC
EcoT22I 4135 ATGCAT ATGCA^T
KspI 3209 CCGCGG CCGC^GG
NdeI 7870 CATATG CA^TATG
NsiI 4135 ATGCAT ATGCA^T
PacI 7986 TTAATTAA TTAAT^TAA
PaeR7I 1540 CTCGAG C^TCGAG
PmeI 950 GTTTAAAC GTTT^AAAC
SacI 7329 GAGCTC GAGCT^C
SacII 3209 CCGCGG CCGC^GG
ScaI 449 AGTACT AGT^ACT
SexAI 7775 ACCTGGT A^CC[AT]GGT
SmaI 10594 CCCGGG CCC^GGG
SnaBI 6968 TACGTA TAC^GTA
SpeI 10372 ACTAGT A^CTAGT
SphI 6502 GCATGC GCATG^C
SspI 5511 AATATT AAT^ATT
XhoI 1540 CTCGAG C^TCGAG
XmaCI 10594 CCCGGG C^CCGGG
XmaI 10594 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 8118 GGTACC G^GTACC
8872 GGTACC
AflIII 9877 ACATGT A^C[GA][CT]GT
9925 ACATGT
ApaI 1381 GGGCCC GGGCC^C
9168 GGGCCC
Asp718I 8118 GGTACC G^GTACC
8872 GGTACC
AspI 2910 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5427 GACAAAGTC
BalI 2885 TGGCCA TGG^CCA
4779 TGGCCA
BbeI 2470 GGCGCC GGCGC^C
9060 GGCGCC
BbvCI 7474 CCTCAGC CCTCAGC(-5/-2)
8636 CCTCAGC
BcgI 3920 CGACACCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
7493 CGAGGGGTCTGC
BsaAI 6968 TACGTA [CT]AC^GT[GA]
9339 TACGTG
BsaBI 680 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6470 GATGAGAATC
BsmBI 568 CGTCTC CGTCTC(1/5)
4818 CGTCTC
BspHI 1033 TCATGA T^CATGA
9771 TCATGA
BspLU11I 9877 ACATGT A^CATGT
9925 ACATGT
BsrBI 7484 CCGCTC CCGCTC(-3/-3)
8856 GAGCGG
BssSI 9452 CACGAG CACGAG(-5/-1)
1122 CTCGTG
BstAPI 1472 GCATTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2390 GCAGTATGTGC
EaeI 2885 TGGCCA [CT]^GGCC[GA]
4779 TGGCCA
EcoNI 8636 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
10251 CCTTCAGAAGG
KasI 2470 GGCGCC G^GCGCC
9060 GGCGCC
KpnI 8118 GGTACC GGTAC^C
8872 GGTACC
MamI 680 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6470 GATGAGAATC
MluNI 2885 TGGCCA TGG^CCA
4779 TGGCCA
MscI 2885 TGGCCA TGG^CCA
4779 TGGCCA
NaeI 5113 GCCGGC GCC^GGC
8907 GCCGGC
NarI 2470 GGCGCC GG^CGCC
9060 GGCGCC
NgoMIV 5113 GCCGGC G^CCGGC
8907 GCCGGC
PflFI 2910 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5427 GACAAAGTC
RcaI 1033 TCATGA T^CATGA
9771 TCATGA
SapI 4987 GCTCTTC GCTCTTC(1/4)
9224 GCTCTTC
SfoI 2470 GGCGCC GGC^GCC
9060 GGCGCC
StuI 7293 AGGCCT AGG^CCT
10815 AGGCCT
Tth111I 2910 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5427 GACAAAGTC
XbaI 8552 TCTAGA T^CTAGA
10055 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AccIII AciI AclI AcsI
AcyI AfaI AluI Alw44I AlwI
AlwNI ApaLI ApoI AseI AsnI
Asp700I AspHI AvaI AvaII BamHI
BanI BanII BbsI BbvI BciVI
BclI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspEI
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EarI EcoO109I EcoRI EcoRII
EcoRV EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MroI MseI MslI MspA1I MspI
MunI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI Psp1406I
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI