##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AgeI AhdI ApoI AscI AseI
AsnI Asp718I AspEI BciVI BsaWI
BseAI BsiWI BspEI Bst1107I BstZ17I
CpoI Eam1105I EcoRI EcoT22I HpaI
KpnI MfeI MluI MroI MunI
NdeI NotI NsiI PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SgrAI SnaBI SpeI SphI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 22 enzymes) #
###################################
AatII 3956 GACGTC GACGT^C
BbrPI 8729 CACGTG CAC^GTG
BcgI 523 GCAAGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1234 TGATCA T^GATCA
BsaBI 2243 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 1650 ATCGAT AT^CGAT
BspLU11I 6742 ACATGT A^CATGT
BssHII 114 GCGCGC G^CGCGC
BstBI 8604 TTCGAA TT^CGAA
ClaI 1650 ATCGAT AT^CGAT
DraI 9005 TTTAAA TTT^AAA
EcoRV 3789 GATATC GAT^ATC
FbaI 1234 TGATCA T^GATCA
HincII 3117 GTCAAC GT[CT]^[GA]AC
HindII 3117 GTCAAC GT[CT]^[GA]AC
MamI 2243 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NruI 6758 TCGCGA TCG^CGA
NspV 8604 TTCGAA TT^CGAA
PmaCI 8729 CACGTG CAC^GTG
PmlI 8729 CACGTG CAC^GTG
ScaI 4342 AGTACT AGT^ACT
SfuI 8604 TTCGAA TT^CGAA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflII 2520 CTTAAG C^TTAAG
8415 CTTAAG
AflIII 6742 ACATGT A^C[GA][CT]GT
7810 ACACGT
Aor51HI 4109 AGCGCT AGC^GCT
8480 AGCGCT
AviII 1024 TGCGCA TGC^GCA
4904 TGCGCA
BamHI 358 GGATCC G^GATCC
8267 GGATCC
BfrI 2520 CTTAAG C^TTAAG
8415 CTTAAG
BglII 502 AGATCT A^GATCT
3643 AGATCT
BsmI 1586 GAATGC GAATGC(1/-1)
4423 GCATTC
Bsp1407I 9791 TGTACA T^GTACA
9827 TGTACA
BsrGI 9791 TGTACA T^GTACA
9827 TGTACA
BssSI 846 CACGAG CACGAG(-5/-1)
4686 CTCGTG
BstEII 4658 GGTCACC G^GT[ACGT]ACC
5741 GGTTACC
BstPI 4658 GGTCACC G^GT[ACGT]ACC
5741 GGTTACC
EagI 139 CGGCCG C^GGCCG
5840 CGGCCG
EclXI 139 CGGCCG C^GGCCG
5840 CGGCCG
Eco47III 4109 AGCGCT AGC^GCT
8480 AGCGCT
Eco52I 139 CGGCCG C^GGCCG
5840 CGGCCG
EcoO65I 4658 GGTCACC G^GT[ACGT]ACC
5741 GGTTACC
FseI 136 GGCCGGCC GGCCGG^CC
320 GGCCGGCC
FspI 1024 TGCGCA TGC^GCA
4904 TGCGCA
HindIII 1788 AAGCTT A^AGCTT
8608 AAGCTT
KspI 142 CCGCGG CCGC^GG
6732 CCGCGG
PaeR7I 1091 CTCGAG C^TCGAG
3943 CTCGAG
PshAI 3110 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
7517 GACGGCTGTC
SacII 142 CCGCGG CCGC^GG
6732 CCGCGG
SbfI 3173 CCTGCAGG CCTGCA^GG
9251 CCTGCAGG
SexAI 1045 ACCTGGT A^CC[AT]GGT
6248 ACCAGGT
Sse8387I 3173 CCTGCAGG CCTGCA^GG
9251 CCTGCAGG
SspBI 9791 TGTACA T^GTACA
9827 TGTACA
XbaI 1793 TCTAGA T^CTAGA
6188 TCTAGA
XhoI 1091 CTCGAG C^TCGAG
3943 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 158 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI Asp700I AspHI AspI AvaI
AvaII AvrII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DrdI DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NheI NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SfoI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI