##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Asp718I AspEI AspI AviII BalI
BbeI BbrPI BbvCI BciVI BsaBI
BsiWI BspDI BspLU11I Bst1107I BstBI
BstEII BstPI BstZ17I ClaI DraI
Eam1105I EcoNI EcoO65I EcoRI FseI
FspI HpaI KasI KpnI MamI
MluI MluNI MscI NarI NheI
NotI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI SalI SbfI ScaI SexAI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XcmI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 4355 AAATTC [GA]^AATT[CT]
AflIII 632 ACGTGT A^C[GA][CT]GT
ApoI 4355 AAATTC [GA]^AATT[CT]
AscI 3001 GGCGCGCC GG^CGCGCC
AseI 4498 ATTAAT AT^TAAT
AsnI 4498 ATTAAT AT^TAAT
Asp700I 2518 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 4040 CCTAGG C^CTAGG
BamHI 3452 GGATCC G^GATCC
BanI 3038 GGTGCC G^G[CT][GA]CC
BcgI 2497 GCAATGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5179 TGATCA T^GATCA
BglII 4192 AGATCT A^GATCT
BlnI 4040 CCTAGG C^CTAGG
BlpI 3872 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3872 GCTGAGC GC^T[ACGT]AGC
BssHII 3002 GCGCGC G^CGCGC
CelII 3872 GCTGAGC GC^T[ACGT]AGC
CpoI 1857 CGGACCG CG^G[AT]CCG
DraIII 3726 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3935 GACTCCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 2178 ATGCAT ATGCA^T
FbaI 5179 TGATCA T^GATCA
MfeI 4293 CAATTG C^AATTG
MunI 4293 CAATTG C^AATTG
NdeI 4209 CATATG CA^TATG
NruI 5225 TCGCGA TCG^CGA
NsiI 2178 ATGCAT ATGCA^T
PaeR7I 3095 CTCGAG C^TCGAG
PshBI 4498 ATTAAT AT^TAAT
RsrII 1857 CGGACCG CG^G[AT]CCG
SphI 3796 GCATGC GCATG^C
StuI 2477 AGGCCT AGG^CCT
XbaI 1115 TCTAGA T^CTAGA
XhoI 3095 CTCGAG C^TCGAG
XmnI 2518 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccIII 1040 TCCGGA T^CCGGA
1253 TCCGGA
AcyI 650 GACGCC G[GA]^CG[CT]C
1967 GACGCC
BsaAI 3051 TACGTG [CT]AC^GT[GA]
4713 TACGTG
BsaHI 650 GACGCC G[GA]^CG[CT]C
1967 GACGCC
BseAI 1040 TCCGGA T^CCGGA
1253 TCCGGA
BsmI 2813 GAATGC GAATGC(1/-1)
3313 GCATTC
BspEI 1040 TCCGGA T^CCGGA
1253 TCCGGA
BspHI 2693 TCATGA T^CATGA
4413 TCATGA
BsrDI 2497 GCAATG GCAATG(2/0)
4609 GCAATG
BssSI 2994 CACGAG CACGAG(-5/-1)
3651 CACGAG
EcoRV 1106 GATATC GAT^ATC
3987 GATATC
Hin1I 650 GACGCC G[GA]^CG[CT]C
1967 GACGCC
HindIII 4453 AAGCTT A^AGCTT
5076 AAGCTT
MroI 1040 TCCGGA T^CCGGA
1253 TCCGGA
NcoI 295 CCATGG C^CATGG
317 CCATGG
PflMI 2079 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2330 CCATGACCTGG
RcaI 2693 TCATGA T^CATGA
4413 TCATGA
SacI 1243 GAGCTC GAGCT^C
3152 GAGCTC
SfiI 389 GGCCGGCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1375 GGCCACCCAGGCC
TfiI 873 GAATC G^A[AT]TC
2919 GAATC
Van91I 2079 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2330 CCATGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AccII AciI AfaI AflII
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BfrI BglI BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacII SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspBI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI