##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccIII AcyI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AspI AvaI AviII BamHI
BbeI BbrPI BcgI BclI BglII
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BsrDI BsrFI BssHII BstAPI
BstBI BstEII BstPI BstXI CfoI
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KspI MamI MluI MroI NaeI
NarI NcoI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PstI
PvuI PvuII RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI Sse8387I SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1827 GGTACC G^GTACC
AclI 1748 AACGTT AA^CGTT
Asp700I 2105 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1827 GGTACC G^GTACC
AvrII 2270 CCTAGG C^CTAGG
BbvCI 1777 CCTCAGC CCTCAGC(-5/-2)
BciVI 1071 GGATAC GTATCC(6/5)
BglI 1359 GCCTGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2270 CCTAGG C^CTAGG
BlpI 450 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 450 GCTCAGC GC^T[ACGT]AGC
BsaAI 3248 TACGTA [CT]AC^GT[GA]
BsaI 1031 GGTCTC GGTCTC(1/5)
BseRI 3149 GAGGAG GAGGAG(10/8)
BsmI 2077 GCATTC GAATGC(1/-1)
BspMI 22 ACCTGC ACCTGC(4/8)
BsrBI 1616 GAGCGG CCGCTC(-3/-3)
BssSI 2790 CACGAG CACGAG(-5/-1)
Bst1107I 2796 GTATAC GTA^TAC
BstYI 2043 GGATCT [GA]^GATC[CT]
BstZ17I 2796 GTATAC GTA^TAC
CelII 450 GCTCAGC GC^T[ACGT]AGC
HgaI 1506 GACGC GACGC(5/10)
HindIII 473 AAGCTT A^AGCTT
KpnI 1827 GGTACC GGTAC^C
MfeI 2128 CAATTG C^AATTG
MflI 2043 GGATCT [GA]^GATC[CT]
MspA1I 866 CAGCGG C[AC]G^C[GT]G
MunI 2128 CAATTG C^AATTG
NdeI 1808 CATATG CA^TATG
NheI 2410 GCTAGC G^CTAGC
PflMI 4242 CCAATATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1748 AACGTT AA^CGTT
SacI 2345 GAGCTC GAGCT^C
ScaI 753 AGTACT AGT^ACT
SnaBI 3248 TACGTA TAC^GTA
SpeI 1204 ACTAGT A^CTAGT
Van91I 4242 CCAATATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1407 CCATGTTCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2043 GGATCT [GA]^GATC[CT]
XmnI 2105 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 1210 CGCG CG^CG
1878 CGCG
AlwI 954 GGATC GGATC(4/5)
2043 GGATC
AlwNI 866 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
3138 CAGCCCCTG
AocI 3765 CCTAAGG CC^T[ACGT]AGG
4364 CCTTAGG
AspHI 2345 GAGCTC G[AT]GC[AT]^C
3455 GTGCTC
BalI 1515 TGGCCA TGG^CCA
3588 TGGCCA
BanII 735 GAGCCC G[GA]GC[CT]^C
2345 GAGCTC
BcnI 3432 CCGGG CC^[GC]GG
4416 CCCGG
BsiHKAI 2345 GAGCTC G[AT]GC[AT]^C
3455 GTGCTC
Bsp1407I 2714 TGTACA T^GTACA
3097 TGTACA
BspHI 567 TCATGA T^CATGA
4552 TCATGA
BsrGI 2714 TGTACA T^GTACA
3097 TGTACA
BstUI 1210 CGCG CG^CG
1878 CGCG
Bsu36I 3765 CCTAAGG CC^T[ACGT]AGG
4364 CCTTAGG
EarI 2770 CTCTTC CTCTTC(1/4)
915 GAAGAG
Eco81I 3765 CCTAAGG CC^T[ACGT]AGG
4364 CCTTAGG
FauI 1368 GCGGG CCCGC(4/6)
2997 GCGGG
Ksp632I 2770 CTCTTC CTCTTC(1/4)
915 GAAGAG
MluNI 1515 TGGCCA TGG^CCA
3588 TGGCCA
MscI 1515 TGGCCA TGG^CCA
3588 TGGCCA
MvnI 1210 CGCG CG^CG
1878 CGCG
NciI 3432 CCGGG CC^[GC]GG
4416 CCCGG
RcaI 567 TCATGA T^CATGA
4552 TCATGA
SspBI 2714 TGTACA T^GTACA
3097 TGTACA
Tsp45I 17 GTGAC ^GT[GC]AC
1621 GTGAC
XbaI 1221 TCTAGA T^CTAGA
1929 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI ApoI AseI AsnI
AvaII BanI BbsI BbvI BfaI
BfrI BmyI BpmI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI EcoNI EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I