##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AflII AflIII AgeI AscI AseI
AsnI AviII BbeI BcgI BfrI
BsaI BsiWI BspLU11I BstBI BstEII
BstPI CpoI EcoO65I EcoT22I FspI
KasI KspI MluI NarI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PshBI PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 3055 GACGTC GACGT^C
AclI 4962 AACGTT AA^CGTT
AcyI 3055 GACGTC G[GA]^CG[CT]C
AocI 3748 CCTGAGG CC^T[ACGT]AGG
BamHI 1731 GGATCC G^GATCC
BclI 2472 TGATCA T^GATCA
BsaBI 1732 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3055 GACGTC G[GA]^CG[CT]C
Bsp1407I 4479 TGTACA T^GTACA
BsrGI 4479 TGTACA T^GTACA
BssHII 3270 GCGCGC G^CGCGC
Bst1107I 506 GTATAC GTA^TAC
BstAPI 2581 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 506 GTATAC GTA^TAC
Bsu36I 3748 CCTGAGG CC^T[ACGT]AGG
DrdI 2279 GACCCAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3748 CCTGAGG CC^T[ACGT]AGG
EcoRI 1493 GAATTC G^AATTC
EcoRV 3594 GATATC GAT^ATC
FbaI 2472 TGATCA T^GATCA
FseI 4280 GGCCGGCC GGCCGG^CC
Hin1I 3055 GACGTC G[GA]^CG[CT]C
HindIII 2452 AAGCTT A^AGCTT
HpaI 2480 GTTAAC GTT^AAC
MamI 1732 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4586 CAATTG C^AATTG
MunI 4586 CAATTG C^AATTG
NdeI 20 CATATG CA^TATG
NspI 4603 ACATGC [GA]CATG^[CT]
PmeI 640 GTTTAAAC GTTT^AAAC
Psp1406I 4962 AACGTT AA^CGTT
SapI 2381 GAAGAGC GCTCTTC(1/4)
SmaI 752 CCCGGG CCC^GGG
SspBI 4479 TGTACA T^GTACA
StuI 77 AGGCCT AGG^CCT
SwaI 3856 ATTTAAAT ATTT^AAAT
XbaI 1951 TCTAGA T^CTAGA
XmaCI 752 CCCGGG C^CCGGG
XmaI 752 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 845 GGTACC G^GTACC
2628 GGTACC
AccI 506 GTATAC GT^[AC][GT]AC
4009 GTAGAC
AccIII 2551 TCCGGA T^CCGGA
3284 TCCGGA
AcsI 1493 GAATTC [GA]^AATT[CT]
4557 AAATTC
AhdI 1257 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3320 GACACCCAGTC
Aor51HI 2983 AGCGCT AGC^GCT
4901 AGCGCT
ApaI 2820 GGGCCC GGGCC^C
3355 GGGCCC
ApoI 1493 GAATTC [GA]^AATT[CT]
4557 AAATTC
Asp718I 845 GGTACC G^GTACC
2628 GGTACC
AspEI 1257 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3320 GACACCCAGTC
AspI 1898 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2032 GACTCTGTC
AvrII 889 CCTAGG C^CTAGG
2665 CCTAGG
BbsI 2276 GAAGAC GAAGAC(2/6)
2801 GAAGAC
BbvCI 3360 CCTCAGC CCTCAGC(-5/-2)
5072 GCTGAGG
BciVI 3642 GGATAC GTATCC(6/5)
2565 GTATCC
BglI 2334 GCCACGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3374 GCCAGCCAGGC
BglII 953 AGATCT A^GATCT
1120 AGATCT
BlnI 889 CCTAGG C^CTAGG
2665 CCTAGG
BpuAI 2276 GAAGAC GAAGAC(2/6)
2801 GAAGAC
BseAI 2551 TCCGGA T^CCGGA
3284 TCCGGA
BsiEI 4283 CGGCCG CG[GA][CT]^CG
5188 CGGCCG
BspDI 3810 ATCGAT AT^CGAT
4022 ATCGAT
BspEI 2551 TCCGGA T^CCGGA
3284 TCCGGA
ClaI 3810 ATCGAT AT^CGAT
4022 ATCGAT
EagI 4283 CGGCCG C^GGCCG
5188 CGGCCG
Eam1105I 1257 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3320 GACACCCAGTC
EclXI 4283 CGGCCG C^GGCCG
5188 CGGCCG
Eco47III 2983 AGCGCT AGC^GCT
4901 AGCGCT
Eco52I 4283 CGGCCG C^GGCCG
5188 CGGCCG
HgaI 2493 GCGTC GACGC(5/10)
3339 GCGTC
KpnI 845 GGTACC GGTAC^C
2628 GGTACC
MroI 2551 TCCGGA T^CCGGA
3284 TCCGGA
NaeI 3099 GCCGGC GCC^GGC
4281 GCCGGC
NgoMIV 3099 GCCGGC G^CCGGC
4281 GCCGGC
NheI 1931 GCTAGC G^CTAGC
4904 GCTAGC
PflFI 1898 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2032 GACTCTGTC
SacI 2067 GAGCTC GAGCT^C
3010 GAGCTC
SexAI 2729 ACCTGGT A^CC[AT]GGT
4004 ACCTGGT
Tth111I 1898 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2032 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI AlwNI ApaLI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PmaCI
PmlI PpuMI PshAI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI