##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII AgeI Aor51HI ApaI AscI
BamHI BbrPI BglI BsiWI BssHII
BstEII BstPI CpoI DraII DrdI
Eco47III EcoNI EcoO109I EcoO65I FseI
MfeI MluI MunI NdeI NotI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PvuI RsrII SalI
SbfI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 5606 TCCGGA T^CCGGA
AclI 3218 AACGTT AA^CGTT
AflII 3711 CTTAAG C^TTAAG
AhdI 7747 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 6107 GTGCAC G^TGCAC
AocI 6029 CCTCAGG CC^T[ACGT]AGG
ApaLI 6107 GTGCAC G^TGCAC
AspEI 7747 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 845 TGCGCA TGC^GCA
BbeI 7628 GGCGCC GGCGC^C
BbvCI 7656 GCTGAGG CCTCAGC(-5/-2)
BcgI 6248 GCAGACGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3711 CTTAAG C^TTAAG
BsaAI 2770 TACGTG [CT]AC^GT[GA]
BseAI 5606 TCCGGA T^CCGGA
BsiEI 16 CGGCCG CG[GA][CT]^CG
BspDI 3760 ATCGAT AT^CGAT
BspEI 5606 TCCGGA T^CCGGA
BsrBI 3414 GAGCGG CCGCTC(-3/-3)
Bst1107I 3531 GTATAC GTA^TAC
BstAPI 4771 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3531 GTATAC GTA^TAC
Bsu36I 6029 CCTCAGG CC^T[ACGT]AGG
ClaI 3760 ATCGAT AT^CGAT
EagI 16 CGGCCG C^GGCCG
Eam1105I 7747 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 16 CGGCCG C^GGCCG
Eco52I 16 CGGCCG C^GGCCG
Eco81I 6029 CCTCAGG CC^T[ACGT]AGG
EcoT22I 6420 ATGCAT ATGCA^T
FspI 845 TGCGCA TGC^GCA
HpaI 6729 GTTAAC GTT^AAC
KasI 7628 GGCGCC G^GCGCC
KspI 6290 CCGCGG CCGC^GG
MroI 5606 TCCGGA T^CCGGA
NaeI 18 GCCGGC GCC^GGC
NarI 7628 GGCGCC GG^CGCC
NgoMIV 18 GCCGGC G^CCGGC
NheI 4728 GCTAGC G^CTAGC
NruI 6389 TCGCGA TCG^CGA
NsiI 6420 ATGCAT ATGCA^T
Psp1406I 3218 AACGTT AA^CGTT
SacII 6290 CCGCGG CCGC^GG
SexAI 3458 ACCTGGT A^CC[AT]GGT
SfoI 7628 GGCGCC GGC^GCC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 568 GGTACC G^GTACC
4090 GGTACC
AccII 6291 CGCG CG^CG
6390 CGCG
AcyI 104 GGCGTC G[GA]^CG[CT]C
7628 GGCGCC
AseI 1519 ATTAAT AT^TAAT
3798 ATTAAT
AsnI 1519 ATTAAT AT^TAAT
3798 ATTAAT
Asp718I 568 GGTACC G^GTACC
4090 GGTACC
AspI 1330 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5893 GACTATGTC
AvaI 3374 CTCGGG C^[CT]CG[GA]G
7648 CTCGGG
AvaII 2751 GGACC G^G[AT]CC
2758 GGTCC
AvrII 286 CCTAGG C^CTAGG
6093 CCTAGG
BclI 6780 TGATCA T^GATCA
7328 TGATCA
BcnI 1538 CCCGG CC^[GC]GG
7613 CCGGG
BglII 1936 AGATCT A^GATCT
4596 AGATCT
BlnI 286 CCTAGG C^CTAGG
6093 CCTAGG
BlpI 1776 GCTTAGC GC^T[ACGT]AGC
5932 GCTCAGC
Bpu1102I 1776 GCTTAGC GC^T[ACGT]AGC
5932 GCTCAGC
BsaHI 104 GGCGTC G[GA]^CG[CT]C
7628 GGCGCC
BsaWI 5606 TCCGGA [AT]^CCGG[AT]
6801 TCCGGT
BsmBI 6717 CGTCTC CGTCTC(1/5)
915 GAGACG
BsoBI 3374 CTCGGG C^[CT]CG[GA]G
7648 CTCGGG
BspLU11I 902 ACATGT A^CATGT
7196 ACATGT
BsrFI 18 GCCGGC [GA]^CCGG[CT]
4019 ACCGGC
BstUI 6291 CGCG CG^CG
6390 CGCG
BstXI 6155 CCAACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7506 CCAGCACTTTGG
CelII 1776 GCTTAGC GC^T[ACGT]AGC
5932 GCTCAGC
Cfr10I 18 GCCGGC [GA]^CCGG[CT]
4019 ACCGGC
EcoRV 114 GATATC GAT^ATC
3478 GATATC
FbaI 6780 TGATCA T^GATCA
7328 TGATCA
HaeII 2870 GGCGCT [GA]GCGC^[CT]
7628 GGCGCC
Hin1I 104 GGCGTC G[GA]^CG[CT]C
7628 GGCGCC
HindIII 2469 AAGCTT A^AGCTT
2721 AAGCTT
KpnI 568 GGTACC GGTAC^C
4090 GGTACC
MvnI 6291 CGCG CG^CG
6390 CGCG
NciI 1538 CCCGG CC^[GC]GG
7613 CCGGG
PflFI 1330 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5893 GACTATGTC
PshAI 2752 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5056 GACACTGGTC
PshBI 1519 ATTAAT AT^TAAT
3798 ATTAAT
SspI 198 AATATT AAT^ATT
643 AATATT
StuI 2225 AGGCCT AGG^CCT
3704 AGGCCT
Tth111I 1330 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5893 GACTATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI BalI BanI BanII BbsI
BbvI BciVI BfaI BmyI BpmI
BpuAI BsaBI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BspMI BsrDI BsrGI BsrI BssKI
BssSI BstBI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraIII DsaI EaeI EarI
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI NspV
PflMI PleI PstI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SfuI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII
XmnI