##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
Aor51HI AscI AseI AsnI Asp718I
AspI BclI BfrI BsiWI BspDI
BspLU11I BstBI ClaI CpoI DraI
Eco47III EcoRV EcoT22I FbaI HpaI
KpnI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PmeI PshBI Psp1406I
PvuI RsrII SalI SfuI SgrAI
SpeI SphI SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 15 enzymes) #
###################################
AccIII 6505 TCCGGA T^CCGGA
AgeI 6917 ACCGGT A^CCGGT
BseAI 6505 TCCGGA T^CCGGA
BspEI 6505 TCCGGA T^CCGGA
EcoRI 4897 GAATTC G^AATTC
HincII 4255 GTCAAC GT[CT]^[GA]AC
HindII 4255 GTCAAC GT[CT]^[GA]AC
HindIII 5212 AAGCTT A^AGCTT
MroI 6505 TCCGGA T^CCGGA
PinAI 6917 ACCGGT A^CCGGT
SbfI 978 CCTGCAGG CCTGCA^GG
SfiI 668 GGCCAGAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 2083 TACGTA TAC^GTA
Sse8387I 978 CCTGCAGG CCTGCA^GG
XbaI 991 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 4330 GAATTT [GA]^AATT[CT]
4897 GAATTC
ApoI 4330 GAATTT [GA]^AATT[CT]
4897 GAATTC
Asp700I 4893 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6123 GAAGTACTTC
AviII 2184 TGCGCA TGC^GCA
5297 TGCGCA
AvrII 7128 CCTAGG C^CTAGG
7154 CCTAGG
BamHI 3750 GGATCC G^GATCC
6837 GGATCC
BbsI 4551 GAAGAC GAAGAC(2/6)
6198 GAAGAC
BciVI 781 GGATAC GTATCC(6/5)
2086 GTATCC
BglII 6017 AGATCT A^GATCT
6669 AGATCT
BlnI 7128 CCTAGG C^CTAGG
7154 CCTAGG
BpuAI 4551 GAAGAC GAAGAC(2/6)
6198 GAAGAC
BsaBI 1387 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5340 GATGTCCATC
BsaWI 6505 TCCGGA [AT]^CCGG[AT]
6917 ACCGGT
Bsp1407I 4799 TGTACA T^GTACA
5390 TGTACA
BspHI 1673 TCATGA T^CATGA
4910 TCATGA
BsrDI 6303 CATTGC GCAATG(2/0)
4498 GCAATG
BsrGI 4799 TGTACA T^GTACA
5390 TGTACA
Bst1107I 4011 GTATAC GTA^TAC
5355 GTATAC
BstEII 264 GGTGACC G^GT[ACGT]ACC
4128 GGTGACC
BstPI 264 GGTGACC G^GT[ACGT]ACC
4128 GGTGACC
BstZ17I 4011 GTATAC GTA^TAC
5355 GTATAC
EcoO65I 264 GGTGACC G^GT[ACGT]ACC
4128 GGTGACC
FseI 1186 GGCCGGCC GGCCGG^CC
3279 GGCCGGCC
FspI 2184 TGCGCA TGC^GCA
5297 TGCGCA
KspI 192 CCGCGG CCGC^GG
3780 CCGCGG
MamI 1387 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5340 GATGTCCATC
MseI 5411 TTAA T^TAA
7291 TTAA
PshAI 4131 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6914 GACACCGGTC
RcaI 1673 TCATGA T^CATGA
4910 TCATGA
SacI 2101 GAGCTC GAGCT^C
3250 GAGCTC
SacII 192 CCGCGG CCGC^GG
3780 CCGCGG
ScaI 6125 AGTACT AGT^ACT
6560 AGTACT
SspBI 4799 TGTACA T^GTACA
5390 TGTACA
Tru9I 5411 TTAA T^TAA
7291 TTAA
XmnI 4893 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6123 GAAGTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 158 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AhdI AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI AspEI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbrPI BbvCI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PaeR7I
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoI
XhoII XmaCI XmaI