##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
AlwNI Aor51HI ApaLI AscI AviII
BamHI BbeI BbrPI BcgI BglII
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
BstYI Cfr10I ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoT22I FseI FspI HaeII HpaI
KasI KspI MflI MluI MroI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SacII SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SpeI Sse8387I SspBI SwaI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccII 3 CGCG CG^CG
AcyI 5243 GACGCC G[GA]^CG[CT]C
AflIII 3362 ACATGT A^C[GA][CT]GT
ApaI 4422 GGGCCC GGGCC^C
Asp700I 3004 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BsaAI 3046 TACGTA [CT]AC^GT[GA]
BsaBI 3100 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5243 GACGCC G[GA]^CG[CT]C
BsaWI 762 ACCGGA [AT]^CCGG[AT]
BspLU11I 3362 ACATGT A^CATGT
BssSI 1973 CTCGTG CACGAG(-5/-1)
Bst1107I 3049 GTATAC GTA^TAC
BstUI 3 CGCG CG^CG
BstZ17I 3049 GTATAC GTA^TAC
CfoI 5236 GCGC GCG^C
DraII 4421 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 4421 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRV 556 GATATC GAT^ATC
HgaI 5243 GACGC GACGC(5/10)
HhaI 5236 GCGC GCG^C
Hin1I 5243 GACGCC G[GA]^CG[CT]C
HinP1I 5236 GCGC G^CGC
HincII 4201 GTCGAC GT[CT]^[GA]AC
HindII 4201 GTCGAC GT[CT]^[GA]AC
MamI 3100 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1891 CAATTG C^AATTG
MunI 1891 CAATTG C^AATTG
MvnI 3 CGCG CG^CG
PshAI 4194 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 4201 GTCGAC G^TCGAC
SnaBI 3046 TACGTA TAC^GTA
SphI 5232 GCATGC GCATG^C
SspI 3007 AATATT AAT^ATT
XbaI 2768 TCTAGA T^CTAGA
XcmI 3490 CCAGGAAAACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3004 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AhdI 1627 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5534 GACAAGCAGTC
AseI 2839 ATTAAT AT^TAAT
5013 ATTAAT
AsnI 2839 ATTAAT AT^TAAT
5013 ATTAAT
AspEI 1627 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5534 GACAAGCAGTC
AvaI 3161 CTCGGG C^[CT]CG[GA]G
5211 CTCGGG
BbvCI 5337 CCTCAGC CCTCAGC(-5/-2)
4268 GCTGAGG
BclI 4320 TGATCA T^GATCA
4685 TGATCA
BglI 2396 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5207 GCCTCTCGGGC
BlpI 4442 GCTCAGC GC^T[ACGT]AGC
4586 GCTCAGC
Bpu1102I 4442 GCTCAGC GC^T[ACGT]AGC
4586 GCTCAGC
BsmBI 1598 CGTCTC CGTCTC(1/5)
5273 GAGACG
BsoBI 3161 CTCGGG C^[CT]CG[GA]G
5211 CTCGGG
BsrDI 2147 CATTGC GCAATG(2/0)
5142 GCAATG
BstAPI 2301 GCAAAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5166 GCAACCTCTGC
BstBI 382 TTCGAA TT^CGAA
777 TTCGAA
BstEII 1449 GGTGACC G^GT[ACGT]ACC
4610 GGTGACC
BstPI 1449 GGTGACC G^GT[ACGT]ACC
4610 GGTGACC
CelII 4442 GCTCAGC GC^T[ACGT]AGC
4586 GCTCAGC
DsaI 3996 CCACGG C^C[GA][CT]GG
4606 CCACGG
Eam1105I 1627 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5534 GACAAGCAGTC
EcoNI 4553 CCTTGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5423 CCTACAGGAGG
EcoO65I 1449 GGTGACC G^GT[ACGT]ACC
4610 GGTGACC
EcoRI 647 GAATTC G^AATTC
716 GAATTC
FbaI 4320 TGATCA T^GATCA
4685 TGATCA
NdeI 921 CATATG CA^TATG
1987 CATATG
NspV 382 TTCGAA TT^CGAA
777 TTCGAA
PflMI 411 CCATACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4159 CCAGGAGTTGG
PshBI 2839 ATTAAT AT^TAAT
5013 ATTAAT
PstI 1787 CTGCAG CTGCA^G
4307 CTGCAG
SapI 404 GAAGAGC GCTCTTC(1/4)
4034 GCTCTTC
SfuI 382 TTCGAA TT^CGAA
777 TTCGAA
StuI 1825 AGGCCT AGG^CCT
2403 AGGCCT
Van91I 411 CCATACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4159 CCAGGAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AflII AluI AlwI AocI ApoI
Asp718I AspHI AspI AvaII AvrII
BalI BanI BanII BbsI BbvI
BciVI BcnI BfaI BfrI BlnI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BspMI BsrI BssKI BstF5I BstNI
BstXI Bsu36I Cac8I Cfr13I DdeI
DpnII DraI EaeI EarI Eco81I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PleI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I