##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AclI AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI AviII BbrPI
BsaAI BsiWI Bsp1407I BspDI BsrGI
BstBI ClaI CpoI DraIII EagI
EclXI Eco47III Eco52I FseI FspI
HpaI KspI MfeI MunI NaeI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SapI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 1907 GACGTC GACGT^C
AspI 1178 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 97 CCTAGG C^CTAGG
BamHI 2037 GGATCC G^GATCC
BciVI 5442 GGATAC GTATCC(6/5)
BclI 2673 TGATCA T^GATCA
BlnI 97 CCTAGG C^CTAGG
BsaBI 2674 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1473 GAGACC GGTCTC(1/5)
BsiEI 448 CGACCG CG[GA][CT]^CG
BsmBI 503 GAGACG CGTCTC(1/5)
BspLU11I 715 ACATGT A^CATGT
BsrDI 3740 GCAATG GCAATG(2/0)
BssHII 3104 GCGCGC G^CGCGC
BssSI 821 CTCGTG CACGAG(-5/-1)
Bst1107I 1062 GTATAC GTA^TAC
BstEII 3609 GGTAACC G^GT[ACGT]ACC
BstPI 3609 GGTAACC G^GT[ACGT]ACC
BstZ17I 1062 GTATAC GTA^TAC
DraI 3423 TTTAAA TTT^AAA
DrdI 2858 GACAAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3609 GGTAACC G^GT[ACGT]ACC
EcoRV 4598 GATATC GAT^ATC
FbaI 2673 TGATCA T^GATCA
HindIII 2204 AAGCTT A^AGCTT
MamI 2674 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 486 ACGCGT A^CGCGT
NheI 1162 GCTAGC G^CTAGC
PflFI 1178 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 920 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 4405 CCTGCAGG CCTGCA^GG
Sse8387I 4405 CCTGCAGG CCTGCA^GG
SspI 6151 AATATT AAT^ATT
Tth111I 1178 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Acc65I 936 GGTACC G^GTACC
4669 GGTACC
AccI 1062 GTATAC GT^[AC][GT]AC
4053 GTCTAC
AflII 3515 CTTAAG C^TTAAG
4010 CTTAAG
AflIII 486 ACGCGT A^C[GA][CT]GT
715 ACATGT
Asp718I 936 GGTACC G^GTACC
4669 GGTACC
BbvCI 4274 CCTCAGC CCTCAGC(-5/-2)
2456 GCTGAGG
BcgI 2026 GCAGGTATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3176 GCAGGCAGCTCG
BfrI 3515 CTTAAG C^TTAAG
4010 CTTAAG
BglI 159 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4174 GCCTTTTGGGC
BsgI 860 CTGCAC GTGCAG(16/14)
1277 CTGCAC
BsrFI 933 GCCGGT [GA]^CCGG[CT]
3330 ACCGGC
BstAPI 850 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2407 GCAGCGACTGC
Cfr10I 933 GCCGGT [GA]^CCGG[CT]
3330 ACCGGC
EcoRI 1046 GAATTC G^AATTC
3005 GAATTC
EcoT22I 4155 ATGCAT ATGCA^T
5255 ATGCAT
KpnI 936 GGTACC GGTAC^C
4669 GGTACC
NsiI 4155 ATGCAT ATGCA^T
5255 ATGCAT
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AhdI AluI AlwI AlwNI
AocI ApaI ApoI Asp700I AspEI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BsaWI
BseAI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XcmI
XhoII XmaCI XmaI XmnI