##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      Aor51HI     AscI        AseI        AsnI        
Asp718I     BamHI       BcgI        BfrI        BglII       
BseAI       BsiWI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DraI        
Eco47III    EcoRV       EcoT22I     HincII      HindII      
HpaI        KpnI        KspI        MluI        MroI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PmeI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SexAI       SfuI        SnaBI       SpeI        
SspBI       SspI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AgeI         4508  ACCGGT               A^CCGGT
Alw44I        548  GTGCAC               G^TGCAC
ApaLI         548  GTGCAC               G^TGCAC
AviII        2663  TGCGCA               TGC^GCA
BbrPI        3874  CACGTG               CAC^GTG
BciVI         734  GGATAC               GTATCC(6/5)
BclI           49  TGATCA               T^GATCA
BsaAI        3874  CACGTG               [CT]AC^GT[GA]
BsaBI        3449  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        4508  ACCGGT               [AT]^CCGG[AT]
BsrDI        3831  CATTGC               GCAATG(2/0)
BssSI        4520  CACGAG               CACGAG(-5/-1)
BstEII       1447  GGTGACC              G^GT[ACGT]ACC
BstPI        1447  GGTGACC              G^GT[ACGT]ACC
EcoO65I      1447  GGTGACC              G^GT[ACGT]ACC
EcoRI        5406  GAATTC               G^AATTC
FbaI           49  TGATCA               T^GATCA
FseI         2805  GGCCGGCC             GGCCGG^CC
FspI         2663  TGCGCA               TGC^GCA
MamI         3449  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         5222  CAATTG               C^AATTG
MunI         5222  CAATTG               C^AATTG
PinAI        4508  ACCGGT               A^CCGGT
PmaCI        3874  CACGTG               CAC^GTG
PmlI         3874  CACGTG               CAC^GTG
SapI         2551  GCTCTTC              GCTCTTC(1/4)
SbfI            8  CCTGCAGG             CCTGCA^GG
ScaI         1268  AGTACT               AGT^ACT
SgrAI        4507  CACCGGTG             C[GA]^CCGG[CT]G
Sse8387I        8  CCTGCAGG             CCTGCA^GG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcsI         4278  GAATTT               [GA]^AATT[CT]
             5406  GAATTC              
AhdI         1795  GACCCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3743  GACCCTGTGTC         
ApoI         4278  GAATTT               [GA]^AATT[CT]
             5406  GAATTC              
Asp700I      1266  GAAGTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2370  GAACTGTTTC          
AspEI        1795  GACCCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3743  GACCCTGTGTC         
AspI         1222  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4198  GACATAGTC           
AvrII        3491  CCTAGG               C^CTAGG
             5345  CCTAGG              
BlnI         3491  CCTAGG               C^CTAGG
             5345  CCTAGG              
BsmI         2814  GCATTC               GAATGC(1/-1)
             5374  GCATTC              
DraIII        948  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1153  CACCGCGTG           
DrdI         1107  GACGCAGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1432  GACCCGGCTGTC        
Eam1105I     1795  GACCCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3743  GACCCTGTGTC         
PflFI        1222  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4198  GACATAGTC           
PshAI         913  GACAGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             5449  GACTCTTGTC          
SacI         2856  GAGCTC               GAGCT^C
             4848  GAGCTC              
SfiI         1164  GGCCCAGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2524  GGCCAACCCGGCC       
Tth111I      1222  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4198  GACATAGTC           
XmnI         1266  GAAGTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2370  GAACTGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  145 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApaI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbeI        BbsI        BbvCI       BbvI        
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EagI        
EarI        EclXI       Eco52I      Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        SmlI        SphI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmaCI       XmaI