##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AflII AscI Asp718I
AspI AvrII BamHI BcgI BfrI
BglII BlnI BsaBI BsiWI Bsp1407I
BspDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI EagI EclXI Eco52I EcoO65I
EcoRV EcoT22I FseI HpaI KpnI
MamI MluI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmeI RsrII SalI SbfI
ScaI SfiI SfuI SmaI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1466 GTCTAC GT^[AC][GT]AC
AclI 1845 AACGTT AA^CGTT
AflIII 1006 ACATGT A^C[GA][CT]GT
AgeI 4448 ACCGGT A^CCGGT
AhdI 1005 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 5263 ATTAAT AT^TAAT
AsnI 5263 ATTAAT AT^TAAT
AspEI 1005 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3793 TGCGCA TGC^GCA
BbeI 3770 GGCGCC GGCGC^C
BbsI 1013 GTCTTC GAAGAC(2/6)
BciVI 1210 GTATCC GTATCC(6/5)
BpuAI 1013 GTCTTC GAAGAC(2/6)
BsmBI 3137 GAGACG CGTCTC(1/5)
BspLU11I 1006 ACATGT A^CATGT
BssSI 2954 CTCGTG CACGAG(-5/-1)
BstAPI 4808 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 2303 GACCCAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1005 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 5099 GAATTC G^AATTC
FspI 3793 TGCGCA TGC^GCA
HgaI 204 GCGTC GACGC(5/10)
HincII 3582 GTCAAC GT[CT]^[GA]AC
HindII 3582 GTCAAC GT[CT]^[GA]AC
HindIII 1201 AAGCTT A^AGCTT
KasI 3770 GGCGCC G^GCGCC
KspI 1114 CCGCGG CCGC^GG
NarI 3770 GGCGCC GG^CGCC
PinAI 4448 ACCGGT A^CCGGT
PshAI 1825 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5263 ATTAAT AT^TAAT
Psp1406I 1845 AACGTT AA^CGTT
PvuI 1906 CGATCG CGAT^CG
SacII 1114 CCGCGG CCGC^GG
SapI 1790 GCTCTTC GCTCTTC(1/4)
SexAI 2349 ACCTGGT A^CC[AT]GGT
SfoI 3770 GGCGCC GGC^GCC
SgrAI 4447 CACCGGTG C[GA]^CCGG[CT]G
SnaBI 3809 TACGTA TAC^GTA
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 3537 TCCGGA T^CCGGA
3826 TCCGGA
AcyI 203 GGCGTC G[GA]^CG[CT]C
3770 GGCGCC
Aor51HI 714 AGCGCT AGC^GCT
2130 AGCGCT
Asp700I 2014 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5261 GAATTAATTC
BanI 1868 GGCACC G^G[CT][GA]CC
3770 GGCGCC
BbrPI 818 CACGTG CAC^GTG
1124 CACGTG
BfaI 698 CTAG C^TAG
5073 CTAG
BlpI 2074 GCTGAGC GC^T[ACGT]AGC
3521 GCTGAGC
Bpu1102I 2074 GCTGAGC GC^T[ACGT]AGC
3521 GCTGAGC
BsaHI 203 GGCGTC G[GA]^CG[CT]C
3770 GGCGCC
BseAI 3537 TCCGGA T^CCGGA
3826 TCCGGA
BsiEI 538 CGACCG CG[GA][CT]^CG
1906 CGATCG
BsmI 1747 GAATGC GAATGC(1/-1)
4698 GCATTC
BspEI 3537 TCCGGA T^CCGGA
3826 TCCGGA
BspHI 861 TCATGA T^CATGA
1642 TCATGA
BstYI 1195 GGATCT [GA]^GATC[CT]
5075 AGATCC
CelII 2074 GCTGAGC GC^T[ACGT]AGC
3521 GCTGAGC
DraIII 1124 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
4436 CACCCAGTG
EarI 1791 CTCTTC CTCTTC(1/4)
5107 CTCTTC
Eco47III 714 AGCGCT AGC^GCT
2130 AGCGCT
EcoNI 2980 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3127 CCTCTGGGAGG
Hin1I 203 GGCGTC G[GA]^CG[CT]C
3770 GGCGCC
Ksp632I 1791 CTCTTC CTCTTC(1/4)
5107 CTCTTC
MaeI 698 CTAG C^TAG
5073 CTAG
MfeI 3405 CAATTG C^AATTG
4457 CAATTG
MflI 1195 GGATCT [GA]^GATC[CT]
5075 AGATCC
MroI 3537 TCCGGA T^CCGGA
3826 TCCGGA
MseI 5193 TTAA T^TAA
5264 TTAA
MunI 3405 CAATTG C^AATTG
4457 CAATTG
NaeI 639 GCCGGC GCC^GGC
1142 GCCGGC
NgoMIV 639 GCCGGC G^CCGGC
1142 GCCGGC
PmaCI 818 CACGTG CAC^GTG
1124 CACGTG
PmlI 818 CACGTG CAC^GTG
1124 CACGTG
RcaI 861 TCATGA T^CATGA
1642 TCATGA
SacI 1982 GAGCTC GAGCT^C
2249 GAGCTC
SphI 594 GCATGC GCATG^C
4326 GCATGC
Tru9I 5193 TTAA T^TAA
5264 TTAA
XhoII 1195 GGATCT [GA]^GATC[CT]
5075 AGATCC
XmnI 2014 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5261 GAATTAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI ApoI AspHI AvaI AvaII
BalI BanII BbvCI BbvI BclI
BcnI BglI BmyI BpmI BsaAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI