##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AgeI AhdI AocI
AscI Asp718I AspEI AviII AvrII
BcgI BciVI BglII BlnI BlpI
Bpu1102I BsaBI BsiWI BsmI Bsu36I
CelII CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I FseI
FspI HindIII KpnI MamI NheI
NotI NruI PacI PaeR7I PflMI
PinAI PmeI PshAI PvuI RsrII
SalI SapI SbfI SexAI SfiI
SnaBI SpeI SphI Sse8387I StuI
Van91I XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 1339 TCCGGA T^CCGGA
Alw44I 2252 GTGCAC G^TGCAC
ApaI 2785 GGGCCC GGGCC^C
ApaLI 2252 GTGCAC G^TGCAC
AseI 5469 ATTAAT AT^TAAT
AsnI 5469 ATTAAT AT^TAAT
BalI 4711 TGGCCA TGG^CCA
BamHI 5588 GGATCC G^GATCC
BbrPI 522 CACGTG CAC^GTG
BsaWI 1339 TCCGGA [AT]^CCGG[AT]
BseAI 1339 TCCGGA T^CCGGA
BsmBI 4525 GAGACG CGTCTC(1/5)
BspDI 3174 ATCGAT AT^CGAT
BspEI 1339 TCCGGA T^CCGGA
BsrDI 788 GCAATG GCAATG(2/0)
BssSI 2297 CACGAG CACGAG(-5/-1)
BstAPI 4584 GCAGCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3941 TTCGAA TT^CGAA
ClaI 3174 ATCGAT AT^CGAT
EarI 5649 GAAGAG CTCTTC(1/4)
EcoNI 943 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1205 GATATC GAT^ATC
HpaI 5700 GTTAAC GTT^AAC
Ksp632I 5649 GAAGAG CTCTTC(1/4)
KspI 934 CCGCGG CCGC^GG
MfeI 3533 CAATTG C^AATTG
MluNI 4711 TGGCCA TGG^CCA
MroI 1339 TCCGGA T^CCGGA
MscI 4711 TGGCCA TGG^CCA
MunI 3533 CAATTG C^AATTG
NcoI 583 CCATGG C^CATGG
NspV 3941 TTCGAA TT^CGAA
PmaCI 522 CACGTG CAC^GTG
PmlI 522 CACGTG CAC^GTG
PshBI 5469 ATTAAT AT^TAAT
PvuII 6348 CAGCTG CAG^CTG
SacII 934 CCGCGG CCGC^GG
ScaI 5070 AGTACT AGT^ACT
SfuI 3941 TTCGAA TT^CGAA
SwaI 3348 ATTTAAAT ATTT^AAAT
XbaI 3217 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AclI 1484 AACGTT AA^CGTT
4422 AACGTT
Aor51HI 2226 AGCGCT AGC^GCT
2695 AGCGCT
Asp700I 1138 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3876 GAAGACATTC
AspI 3273 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4527 GACGGAGTC
BclI 2823 TGATCA T^GATCA
3923 TGATCA
BglI 4543 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5170 GCCATCTTGGC
BsaAI 522 CACGTG [CT]AC^GT[GA]
2243 TACGTG
BsgI 1027 GTGCAG GTGCAG(16/14)
5160 GTGCAG
BsiEI 994 CGACCG CG[GA][CT]^CG
2471 CGGTCG
BspLU11I 4141 ACATGT A^CATGT
4498 ACATGT
BsrFI 2410 GCCGGC [GA]^CCGG[CT]
2483 GCCGGC
Bst1107I 1462 GTATAC GTA^TAC
5849 GTATAC
BstEII 1876 GGTGACC G^GT[ACGT]ACC
1996 GGTTACC
BstPI 1876 GGTGACC G^GT[ACGT]ACC
1996 GGTTACC
BstZ17I 1462 GTATAC GTA^TAC
5849 GTATAC
Cfr10I 2410 GCCGGC [GA]^CCGG[CT]
2483 GCCGGC
Eco47III 2226 AGCGCT AGC^GCT
2695 AGCGCT
EcoO65I 1876 GGTGACC G^GT[ACGT]ACC
1996 GGTTACC
EcoT14I 583 CCATGG C^C[AT][AT]GG
4798 CCAAGG
EcoT22I 3474 ATGCAT ATGCA^T
5689 ATGCAT
FbaI 2823 TGATCA T^GATCA
3923 TGATCA
MluI 2535 ACGCGT A^CGCGT
2624 ACGCGT
MslI 850 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1511 CACCCCAATG
NaeI 2410 GCCGGC GCC^GGC
2483 GCCGGC
NgoMIV 2410 GCCGGC G^CCGGC
2483 GCCGGC
NsiI 3474 ATGCAT ATGCA^T
5689 ATGCAT
PflFI 3273 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4527 GACGGAGTC
Psp1406I 1484 AACGTT AA^CGTT
4422 AACGTT
SacI 1956 GAGCTC GAGCT^C
4856 GAGCTC
SgrAI 2409 CGCCGGCG C[GA]^CCGG[CT]G
2482 CGCCGGCG
StyI 583 CCATGG C^C[AT][AT]GG
4798 CCAAGG
Tth111I 3273 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4527 GACGGAGTC
XcmI 1596 CCAAGTTCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2040 CCAGGGTGTCTGTGG
XmnI 1138 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3876 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AflIII AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BfrI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspHI
BspMI BsrBI BsrGI BsrI BssHII
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EcoO109I EcoRI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI SspBI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI