##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
AscI AspEI AviII AvrII BcgI
BfrI BlnI BsiEI BsiWI Bsp1407I
BspHI BsrGI BssHII Bst1107I BstAPI
BstBI BstZ17I CpoI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV EcoT22I
FseI FspI HpaI KspI MfeI
MluI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI Psp1406I
PvuI RcaI RsrII SacII SalI
SexAI SfuI SgrAI SnaBI SpeI
SphI SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 163 CGCG CG^CG
Alw44I 5220 GTGCAC G^TGCAC
Aor51HI 5115 AGCGCT AGC^GCT
ApaLI 5220 GTGCAC G^TGCAC
Asp700I 5631 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2843 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 5223 CACGTG CAC^GTG
BciVI 5276 GTATCC GTATCC(6/5)
BclI 498 TGATCA T^GATCA
BglII 3737 AGATCT A^GATCT
BsaAI 5223 CACGTG [CT]AC^GT[GA]
BsmBI 1278 GAGACG CGTCTC(1/5)
BspDI 3195 ATCGAT AT^CGAT
BspLU11I 4738 ACATGT A^CATGT
BsrFI 5820 GCCGGT [GA]^CCGG[CT]
BssSI 1674 CACGAG CACGAG(-5/-1)
BstEII 1556 GGTCACC G^GT[ACGT]ACC
BstPI 1556 GGTCACC G^GT[ACGT]ACC
BstUI 163 CGCG CG^CG
Cfr10I 5820 GCCGGT [GA]^CCGG[CT]
ClaI 3195 ATCGAT AT^CGAT
DraI 6158 TTTAAA TTT^AAA
Eco47III 5115 AGCGCT AGC^GCT
EcoO65I 1556 GGTCACC G^GT[ACGT]ACC
FbaI 498 TGATCA T^GATCA
HindIII 4240 AAGCTT A^AGCTT
MvnI 163 CGCG CG^CG
PflFI 2843 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5223 CACGTG CAC^GTG
PmlI 5223 CACGTG CAC^GTG
PshAI 1550 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 545 GAAGAGC GCTCTTC(1/4)
SbfI 1396 CCTGCAGG CCTGCA^GG
SfiI 5312 GGCCCCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1396 CCTGCAGG CCTGCA^GG
SspI 3222 AATATT AAT^ATT
StuI 4947 AGGCCT AGG^CCT
Tth111I 2843 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 5069 CCAGCAGCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 5631 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 1861 GGTACC G^GTACC
6063 GGTACC
AccI 3539 GTAGAC GT^[AC][GT]AC
4158 GTAGAC
AflIII 3176 ACACGT A^C[GA][CT]GT
4738 ACATGT
AlwNI 2060 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
3747 CAGGCCCTG
Asp718I 1861 GGTACC G^GTACC
6063 GGTACC
BamHI 1402 GGATCC G^GATCC
2931 GGATCC
BbvCI 1648 CCTCAGC CCTCAGC(-5/-2)
2355 CCTCAGC
BsaBI 1804 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5271 GATGTGTATC
BsrBI 557 GAGCGG CCGCTC(-3/-3)
2045 GAGCGG
KpnI 1861 GGTACC GGTAC^C
6063 GGTACC
MamI 1804 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5271 GATGTGTATC
MslI 1528 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4384 CATCCTAATG
PflMI 4297 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5683 CCACCACCTGG
ScaI 1539 AGTACT AGT^ACT
3555 AGTACT
SmaI 649 CCCGGG CCC^GGG
2034 CCCGGG
TaqI 3181 TCGA T^CGA
3196 TCGA
TthHB8I 3181 TCGA T^CGA
3196 TCGA
Van91I 4297 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5683 CCACCACCTGG
XmaCI 649 CCCGGG C^CCGGG
2034 CCCGGG
XmaI 649 CCCGGG C^CCGGG
2034 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccIII AciI AcsI AcyI AfaI
AluI AlwI AocI ApaI ApoI
AseI AsnI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspEI
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MspA1I MspI MvaI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PshBI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XbaI XhoII