##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AclI AflII AhdI Alw44I
ApaLI AscI AspEI AviII AvrII
BamHI BbeI BbvCI BclI BfrI
BglI BglII BlnI BsaBI BsmI
BspLU11I BssHII BstBI BstEII BstPI
CpoI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HpaI
KasI MamI MluI NarI NheI
NotI NruI NsiI NspV PacI
PmeI PshAI Psp1406I PvuI RsrII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1685 GGTACC G^GTACC
AccIII 4736 TCCGGA T^CCGGA
AcyI 156 GACGCC G[GA]^CG[CT]C
AgeI 884 ACCGGT A^CCGGT
Aor51HI 1804 AGCGCT AGC^GCT
ApaI 1830 GGGCCC GGGCC^C
Asp700I 2672 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1685 GGTACC G^GTACC
BbrPI 1959 CACGTG CAC^GTG
BcgI 534 GCAAAGCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 156 GACGCC G[GA]^CG[CT]C
BseAI 4736 TCCGGA T^CCGGA
BsiWI 5412 CGTACG C^GTACG
BspEI 4736 TCCGGA T^CCGGA
BspHI 1295 TCATGA T^CATGA
Bst1107I 2164 GTATAC GTA^TAC
BstAPI 5142 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2164 GTATAC GTA^TAC
DrdI 469 GACGGAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1804 AGCGCT AGC^GCT
EcoNI 2868 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 156 GACGCC G[GA]^CG[CT]C
KpnI 1685 GGTACC GGTAC^C
KspI 2686 CCGCGG CCGC^GG
MroI 4736 TCCGGA T^CCGGA
NaeI 2807 GCCGGC GCC^GGC
NdeI 5307 CATATG CA^TATG
NgoMIV 2807 GCCGGC G^CCGGC
PaeR7I 397 CTCGAG C^TCGAG
PinAI 884 ACCGGT A^CCGGT
PmaCI 1959 CACGTG CAC^GTG
PmlI 1959 CACGTG CAC^GTG
RcaI 1295 TCATGA T^CATGA
SacII 2686 CCGCGG CCGC^GG
ScaI 6231 AGTACT AGT^ACT
SexAI 821 ACCAGGT A^CC[AT]GGT
StuI 139 AGGCCT AGG^CCT
XbaI 3131 TCTAGA T^CTAGA
XcmI 1864 CCAAAACCAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 397 CTCGAG C^TCGAG
XmnI 2672 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 2164 GTATAC GT^[AC][GT]AC
5661 GTCTAC
AflIII 5768 ACACGT A^C[GA][CT]GT
6000 ACACGT
AocI 4047 CCTGAGG CC^T[ACGT]AGG
4684 CCTCAGG
AseI 873 ATTAAT AT^TAAT
6336 ATTAAT
AsnI 873 ATTAAT AT^TAAT
6336 ATTAAT
BciVI 2034 GGATAC GTATCC(6/5)
4728 GTATCC
BlpI 2333 GCTGAGC GC^T[ACGT]AGC
2382 GCTCAGC
Bpu1102I 2333 GCTGAGC GC^T[ACGT]AGC
2382 GCTCAGC
BsaAI 1959 CACGTG [CT]AC^GT[GA]
6001 CACGTA
BsiEI 319 CGGCCG CG[GA][CT]^CG
366 CGGCCG
Bsp1407I 1519 TGTACA T^GTACA
2290 TGTACA
BsrGI 1519 TGTACA T^GTACA
2290 TGTACA
Bsu36I 4047 CCTGAGG CC^T[ACGT]AGG
4684 CCTCAGG
CelII 2333 GCTGAGC GC^T[ACGT]AGC
2382 GCTCAGC
DraIII 820 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2261 CACCATGTG
EagI 319 CGGCCG C^GGCCG
366 CGGCCG
EclXI 319 CGGCCG C^GGCCG
366 CGGCCG
Eco52I 319 CGGCCG C^GGCCG
366 CGGCCG
Eco81I 4047 CCTGAGG CC^T[ACGT]AGG
4684 CCTCAGG
HincII 1905 GTCAAC GT[CT]^[GA]AC
3724 GTCAAC
HindII 1905 GTCAAC GT[CT]^[GA]AC
3724 GTCAAC
MfeI 1387 CAATTG C^AATTG
4993 CAATTG
MunI 1387 CAATTG C^AATTG
4993 CAATTG
NcoI 426 CCATGG C^CATGG
5742 CCATGG
PflMI 2505 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2630 CCAGTTTGTGG
PshBI 873 ATTAAT AT^TAAT
6336 ATTAAT
SphI 4509 GCATGC GCATG^C
5574 GCATGC
SspBI 1519 TGTACA T^GTACA
2290 TGTACA
Van91I 2505 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2630 CCAGTTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspDI BspMI BsrBI BsrDI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I ClaI DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII
XmaCI XmaI