##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AcsI AflII AgeI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BclI
BfrI BlnI BsiWI BspDI BspLU11I
BstBI BstEII BstPI ClaI CpoI
DraI DrdI Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI KpnI MfeI MluI MunI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
PvuI RsrII SalI SapI ScaI
SfuI SgrAI SnaBI SpeI SspI
SwaI TfiI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 1821 TCCGGA T^CCGGA
AclI 4457 AACGTT AA^CGTT
AhdI 1260 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1260 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2868 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 165 GCAGCATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3372 GGATAC GTATCC(6/5)
BsaBI 1182 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1821 TCCGGA T^CCGGA
Bsp1407I 4294 TGTACA T^GTACA
BspEI 1821 TCCGGA T^CCGGA
BspHI 4939 TCATGA T^CATGA
BsrDI 836 CATTGC GCAATG(2/0)
BsrGI 4294 TGTACA T^GTACA
Bst1107I 4481 GTATAC GTA^TAC
BstAPI 496 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 4481 GTATAC GTA^TAC
Eam1105I 1260 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 4234 GTTGAC GT[CT]^[GA]AC
HindII 4234 GTTGAC GT[CT]^[GA]AC
HindIII 5430 AAGCTT A^AGCTT
MamI 1182 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1821 TCCGGA T^CCGGA
NaeI 3760 GCCGGC GCC^GGC
NgoMIV 3760 GCCGGC G^CCGGC
NheI 433 GCTAGC G^CTAGC
NspI 5062 GCATGC [GA]CATG^[CT]
PflFI 2868 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 801 CCACTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 4457 AACGTT AA^CGTT
RcaI 4939 TCATGA T^CATGA
SbfI 2703 CCTGCAGG CCTGCA^GG
SfiI 1929 GGCCCTGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 5062 GCATGC GCATG^C
Sse8387I 2703 CCTGCAGG CCTGCA^GG
SspBI 4294 TGTACA T^GTACA
Tth111I 2868 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 801 CCACTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5085 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 4481 GTATAC GT^[AC][GT]AC
5181 GTCTAC
Alw44I 2416 GTGCAC G^TGCAC
4333 GTGCAC
AocI 3365 CCTGAGG CC^T[ACGT]AGG
3693 CCTGAGG
ApaLI 2416 GTGCAC G^TGCAC
4333 GTGCAC
BalI 1238 TGGCCA TGG^CCA
1472 TGGCCA
BbvCI 3934 CCTCAGC CCTCAGC(-5/-2)
1465 GCTGAGG
BglII 1376 AGATCT A^GATCT
2486 AGATCT
BsaWI 1821 TCCGGA [AT]^CCGG[AT]
3301 TCCGGT
BsmI 840 GCATTC GAATGC(1/-1)
4509 GCATTC
BssSI 2419 CACGAG CACGAG(-5/-1)
1585 CTCGTG
Bsu36I 3365 CCTGAGG CC^T[ACGT]AGG
3693 CCTGAGG
DraIII 802 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
1174 CACAAAGTG
EarI 1159 CTCTTC CTCTTC(1/4)
1356 CTCTTC
Eco81I 3365 CCTGAGG CC^T[ACGT]AGG
3693 CCTGAGG
Ksp632I 1159 CTCTTC CTCTTC(1/4)
1356 CTCTTC
KspI 9 CCGCGG CCGC^GG
3500 CCGCGG
MluNI 1238 TGGCCA TGG^CCA
1472 TGGCCA
MscI 1238 TGGCCA TGG^CCA
1472 TGGCCA
MseI 3824 TTAA T^TAA
5075 TTAA
NcoI 383 CCATGG C^CATGG
1241 CCATGG
PshAI 4203 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5253 GACGGGTGTC
SacI 1753 GAGCTC GAGCT^C
1783 GAGCTC
SacII 9 CCGCGG CCGC^GG
3500 CCGCGG
SexAI 1126 ACCAGGT A^CC[AT]GGT
4950 ACCTGGT
Tru9I 3824 TTAA T^TAA
5075 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BamHI BanI BanII
BbeI BbrPI BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI