##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AclI AgeI AhdI Aor51HI AscI
AseI AsnI AspEI AspI AvrII
BclI BglI BlnI BlpI Bpu1102I
BsiEI BsiWI BsmBI Bsp1407I BspDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FbaI
FseI HgaI HindIII KspI MfeI
MluI MunI NaeI NgoMIV NotI
NruI NsiI NspV PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccII 376 CGCG CG^CG
AccIII 1203 TCCGGA T^CCGGA
AflII 3532 CTTAAG C^TTAAG
AviII 1731 TGCGCA TGC^GCA
BbrPI 3450 CACGTG CAC^GTG
BbvCI 1318 CCTCAGC CCTCAGC(-5/-2)
BfrI 3532 CTTAAG C^TTAAG
BsaBI 1360 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1203 TCCGGA T^CCGGA
BspEI 1203 TCCGGA T^CCGGA
BspLU11I 2175 ACATGT A^CATGT
BssSI 647 CTCGTG CACGAG(-5/-1)
BstUI 376 CGCG CG^CG
EcoRV 1067 GATATC GAT^ATC
FspI 1731 TGCGCA TGC^GCA
HpaI 2060 GTTAAC GTT^AAC
MamI 1360 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1203 TCCGGA T^CCGGA
MvnI 376 CGCG CG^CG
NdeI 1716 CATATG CA^TATG
NheI 3164 GCTAGC G^CTAGC
PacI 3965 TTAATTAA TTAAT^TAA
PflMI 300 CCACTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3450 CACGTG CAC^GTG
PmlI 3450 CACGTG CAC^GTG
SapI 1840 GCTCTTC GCTCTTC(1/4)
ScaI 2902 AGTACT AGT^ACT
SmlI 3532 CTTAAG C^T[CT][GA]AG
Van91I 300 CCACTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 278 CCATTCTACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1112 GACGTC GACGT^C
1286 GACGTC
AccI 1679 GTCTAC GT^[AC][GT]AC
3473 GTAGAC
AcsI 1999 AAATTT [GA]^AATT[CT]
3723 GAATTT
AflIII 888 ACACGT A^C[GA][CT]GT
2175 ACATGT
Alw44I 967 GTGCAC G^TGCAC
1277 GTGCAC
AlwNI 2760 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
3744 CAGTGACTG
ApaI 58 GGGCCC GGGCC^C
3173 GGGCCC
ApaLI 967 GTGCAC G^TGCAC
1277 GTGCAC
ApoI 1999 AAATTT [GA]^AATT[CT]
3723 GAATTT
Asp700I 1582 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1870 GAATCAGTTC
BamHI 2346 GGATCC G^GATCC
2554 GGATCC
BbeI 233 GGCGCC GGCGC^C
1823 GGCGCC
BcgI 1566 GCATCTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1650 GCATGTGGTTCG
BglII 816 AGATCT A^GATCT
2190 AGATCT
BsaAI 889 CACGTA [CT]AC^GT[GA]
3450 CACGTG
BsaWI 537 ACCGGA [AT]^CCGG[AT]
1203 TCCGGA
BspHI 567 TCATGA T^CATGA
1422 TCATGA
BspMI 699 ACCTGC ACCTGC(4/8)
822 ACCTGC
BsrDI 688 CATTGC GCAATG(2/0)
1213 CATTGC
BsrFI 270 GCCGGT [GA]^CCGG[CT]
789 ACCGGC
BstAPI 88 GCACCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2929 GCAGGAATTGC
Cfr10I 270 GCCGGT [GA]^CCGG[CT]
789 ACCGGC
DraI 1498 TTTAAA TTT^AAA
3941 TTTAAA
DrdI 536 GACCGGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
671 GACTACTACGTC
KasI 233 GGCGCC G^GCGCC
1823 GGCGCC
NarI 233 GGCGCC GG^CGCC
1823 GGCGCC
NcoI 1251 CCATGG C^CATGG
1632 CCATGG
PstI 2281 CTGCAG CTGCA^G
3750 CTGCAG
RcaI 567 TCATGA T^CATGA
1422 TCATGA
SfoI 233 GGCGCC GGC^GCC
1823 GGCGCC
StuI 132 AGGCCT AGG^CCT
2834 AGGCCT
XmnI 1582 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1870 GAATCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
Acc65I AciI AcyI AfaI AluI
AlwI AocI Asp718I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII