##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AclI AgeI AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BalI
BbeI BclI BglI BglII BlnI
BsiWI BsmBI BspDI BspHI BsrBI
BssHII Bst1107I BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DrdI Eam1105I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI FspI HpaI KasI
KpnI KspI MfeI MluNI MscI
MunI NaeI NarI NgoMIV NheI
NotI NruI PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfiI
SfoI SgrAI SpeI Sse8387I SwaI
XbaI XcmI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 6 GACGTC GACGT^C
AccIII 227 TCCGGA T^CCGGA
AcyI 6 GACGTC G[GA]^CG[CT]C
AlwNI 2996 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 12 AGCGCT AGC^GCT
ApaI 3354 GGGCCC GGGCC^C
BamHI 728 GGATCC G^GATCC
BbrPI 1459 CACGTG CAC^GTG
BsaBI 1517 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 6 GACGTC G[GA]^CG[CT]C
BseAI 227 TCCGGA T^CCGGA
BsmI 1944 GCATTC GAATGC(1/-1)
Bsp1407I 2649 TGTACA T^GTACA
BspEI 227 TCCGGA T^CCGGA
BspMI 1616 GCAGGT ACCTGC(4/8)
BsrFI 82 ACCGGC [GA]^CCGG[CT]
BsrGI 2649 TGTACA T^GTACA
BstBI 2730 TTCGAA TT^CGAA
Cfr10I 82 ACCGGC [GA]^CCGG[CT]
DraIII 2139 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 12 AGCGCT AGC^GCT
EcoT14I 96 CCATGG C^C[AT][AT]GG
EcoT22I 302 ATGCAT ATGCA^T
Hin1I 6 GACGTC G[GA]^CG[CT]C
MamI 1517 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 102 ACGCGT A^CGCGT
MroI 227 TCCGGA T^CCGGA
NcoI 96 CCATGG C^CATGG
NsiI 302 ATGCAT ATGCA^T
NspV 2730 TTCGAA TT^CGAA
PflMI 459 CCACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1459 CACGTG CAC^GTG
PmlI 1459 CACGTG CAC^GTG
ScaI 3466 AGTACT AGT^ACT
SexAI 3277 ACCTGGT A^CC[AT]GGT
SfuI 2730 TTCGAA TT^CGAA
SnaBI 3265 TACGTA TAC^GTA
SphI 1171 GCATGC GCATG^C
SspBI 2649 TGTACA T^GTACA
SspI 347 AATATT AAT^ATT
StuI 2391 AGGCCT AGG^CCT
StyI 96 CCATGG C^C[AT][AT]GG
Van91I 459 CCACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
BanI 1414 GGTGCC G^G[CT][GA]CC
3409 GGCACC
BcgI 1045 CGATGACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2907 CGATCAAGTTGC
BciVI 444 GGATAC GTATCC(6/5)
2881 GTATCC
BlpI 1249 GCTCAGC GC^T[ACGT]AGC
1853 GCTGAGC
Bpu1102I 1249 GCTCAGC GC^T[ACGT]AGC
1853 GCTGAGC
BsaAI 1459 CACGTG [CT]AC^GT[GA]
3265 TACGTA
BsgI 2145 GTGCAG GTGCAG(16/14)
3679 GTGCAG
BsiEI 84 CGGCCG CG[GA][CT]^CG
1351 CGGCCG
BspLU11I 2652 ACATGT A^CATGT
3770 ACATGT
BsrDI 1870 CATTGC GCAATG(2/0)
3712 GCAATG
BssSI 1366 CTCGTG CACGAG(-5/-1)
1859 CTCGTG
BstAPI 1408 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2138 GCACAAGGTGC
BstXI 250 CCAGGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
343 CCAAAATATTGG
CelII 1249 GCTCAGC GC^T[ACGT]AGC
1853 GCTGAGC
EagI 84 CGGCCG C^GGCCG
1351 CGGCCG
EclXI 84 CGGCCG C^GGCCG
1351 CGGCCG
Eco52I 84 CGGCCG C^GGCCG
1351 CGGCCG
FauI 36 GCGGG CCCGC(4/6)
43 GCGGG
HaeII 12 AGCGCT [GA]GCGC^[CT]
2295 AGCGCC
HincII 1179 GTCAAC GT[CT]^[GA]AC
1188 GTCAAC
HindII 1179 GTCAAC GT[CT]^[GA]AC
1188 GTCAAC
NdeI 365 CATATG CA^TATG
889 CATATG
PaeR7I 1903 CTCGAG C^TCGAG
2327 CTCGAG
PpuMI 2222 AGGTCCC [GA]G^G[AT]CC[CT]
2406 GGGTCCC
PstI 960 CTGCAG CTGCA^G
1967 CTGCAG
SacI 1247 GAGCTC GAGCT^C
1856 GAGCTC
SmaI 62 CCCGGG CCC^GGG
1884 CCCGGG
XhoI 1903 CTCGAG C^TCGAG
2327 CTCGAG
XmaCI 62 CCCGGG C^CCGGG
1884 CCCGGG
XmaI 62 CCCGGG C^CCGGG
1884 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AflIII AluI AlwI ApoI
Asp700I AspHI AspI AvaI AvaII
BanII BbsI BbvCI BbvI BcnI
BfaI BfrI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PleI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XhoII XmnI