##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccII AccIII AgeI AocI
ApaI AscI AvaI AviII BanII
BseAI BsiEI BsiWI BsoBI BspDI
BspEI BsrBI BsrFI BssHII Bst1107I
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DrdI
EagI EclXI Eco52I Eco81I EcoO65I
FseI FspI KspI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NdeI NgoMIV NotI NruI PacI
PaeR7I PinAI PmeI PshAI PvuI
PvuII RsrII SacI SacII SalI
SbfI SfiI SgrAI SmaI SnaBI
SphI Sse8387I StuI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AclI 3558 AACGTT AA^CGTT
AcyI 5699 GGCGCC G[GA]^CG[CT]C
AhdI 3310 GACTCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3902 AGCGCT AGC^GCT
AseI 6397 ATTAAT AT^TAAT
AsnI 6397 ATTAAT AT^TAAT
AspEI 3310 GACTCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4985 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4969 CCTAGG C^CTAGG
BalI 5578 TGGCCA TGG^CCA
BbeI 5699 GGCGCC GGCGC^C
BbrPI 1939 CACGTG CAC^GTG
BcgI 5268 GCACCCATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1149 GGATAC GTATCC(6/5)
BcnI 742 CCGGG CC^[GC]GG
BlnI 4969 CCTAGG C^CTAGG
BlpI 159 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 159 GCTCAGC GC^T[ACGT]AGC
BsaAI 1939 CACGTG [CT]AC^GT[GA]
BsaBI 166 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5699 GGCGCC G[GA]^CG[CT]C
BsaI 4937 GGTCTC GGTCTC(1/5)
BsaWI 1305 ACCGGA [AT]^CCGG[AT]
BsmBI 3093 GAGACG CGTCTC(1/5)
BsmI 1368 GAATGC GAATGC(1/-1)
BspMI 3933 GCAGGT ACCTGC(4/8)
BssSI 5136 CTCGTG CACGAG(-5/-1)
BstBI 5276 TTCGAA TT^CGAA
CelII 159 GCTCAGC GC^T[ACGT]AGC
DraIII 5939 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3310 GACTCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3902 AGCGCT AGC^GCT
EcoRV 3759 GATATC GAT^ATC
EcoT22I 3485 ATGCAT ATGCA^T
Hin1I 5699 GGCGCC G[GA]^CG[CT]C
HpaI 6454 GTTAAC GTT^AAC
KasI 5699 GGCGCC G^GCGCC
MamI 166 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 5578 TGGCCA TGG^CCA
MscI 5578 TGGCCA TGG^CCA
NarI 5699 GGCGCC GG^CGCC
NciI 742 CCGGG CC^[GC]GG
NheI 2571 GCTAGC G^CTAGC
NsiI 3485 ATGCAT ATGCA^T
NspV 5276 TTCGAA TT^CGAA
PflFI 4985 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1939 CACGTG CAC^GTG
PmlI 1939 CACGTG CAC^GTG
PshBI 6397 ATTAAT AT^TAAT
Psp1406I 3558 AACGTT AA^CGTT
SfoI 5699 GGCGCC GGC^GCC
SfuI 5276 TTCGAA TT^CGAA
SwaI 6161 ATTTAAAT ATTT^AAAT
Tth111I 4985 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3257 CCAATAAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflII 354 CTTAAG C^TTAAG
6776 CTTAAG
AflIII 1866 ACATGT A^C[GA][CT]GT
5323 ACATGT
Alw44I 4124 GTGCAC G^TGCAC
5266 GTGCAC
ApaLI 4124 GTGCAC G^TGCAC
5266 GTGCAC
Asp700I 4270 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6916 GAATTTCTTC
BbvCI 1903 CCTCAGC CCTCAGC(-5/-2)
4252 CCTCAGC
BclI 2121 TGATCA T^GATCA
3036 TGATCA
BfrI 354 CTTAAG C^TTAAG
6776 CTTAAG
BglI 4430 GCCTTTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5691 GCCAGAAAGGC
BglII 385 AGATCT A^GATCT
4864 AGATCT
BpmI 5540 CTCCAG CTGGAG(16/14)
91 CTGGAG
BspHI 765 TCATGA T^CATGA
2700 TCATGA
BspLU11I 1866 ACATGT A^CATGT
5323 ACATGT
BstAPI 2881 GCAAAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3368 GCAAAATCTGC
CfoI 3903 GCGC GCG^C
5700 GCGC
EaeI 3157 CGGCCA [CT]^GGCC[GA]
5578 TGGCCA
EcoNI 175 CCTATGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3717 CCTGACAGAGG
EcoRI 2389 GAATTC G^AATTC
3520 GAATTC
FauI 2030 CCCGC CCCGC(4/6)
5655 CCCGC
FbaI 2121 TGATCA T^GATCA
3036 TGATCA
HaeII 3902 AGCGCT [GA]GCGC^[CT]
5699 GGCGCC
HgaI 1027 GACGC GACGC(5/10)
5855 GACGC
HhaI 3903 GCGC GCG^C
5700 GCGC
HinP1I 3903 GCGC G^CGC
5700 GCGC
PflMI 3694 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5542 CCAGGCTTTGG
RcaI 765 TCATGA T^CATGA
2700 TCATGA
SapI 1948 GAAGAGC GCTCTTC(1/4)
6756 GCTCTTC
SexAI 2691 ACCAGGT A^CC[AT]GGT
5669 ACCAGGT
Van91I 3694 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5542 CCAGGCTTTGG
XmnI 4270 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6916 GAATTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp718I
AspHI AvaII BamHI BanI BbsI
BbvI BfaI BmyI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HindIII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SpeI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII