##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AccI AccIII AcyI AhdI
AscI AseI AsnI AspEI AvrII
BbeI BcgI BlnI BsaBI BsaHI
BseAI BspDI BspEI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI Eam1105I EcoRV FseI Hin1I
HpaI KasI MamI MfeI MluI
MroI MunI NarI NotI NruI
NspV PacI PmeI PshAI PshBI
PvuI RsrII SalI SexAI SfoI
SfuI SnaBI SpeI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 5693 AACGTT AA^CGTT
AcsI 1217 GAATTC [GA]^AATT[CT]
ApoI 1217 GAATTC [GA]^AATT[CT]
Asp700I 1027 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 5175 TGCGCA TGC^GCA
BbrPI 3566 CACGTG CAC^GTG
BbsI 2433 GAAGAC GAAGAC(2/6)
BciVI 3709 GGATAC GTATCC(6/5)
BclI 1927 TGATCA T^GATCA
BglII 5782 AGATCT A^GATCT
BpuAI 2433 GAAGAC GAAGAC(2/6)
BsiWI 5353 CGTACG C^GTACG
BsmI 1129 GAATGC GAATGC(1/-1)
Bsp1407I 5712 TGTACA T^GTACA
BspHI 1624 TCATGA T^CATGA
BsrGI 5712 TGTACA T^GTACA
BssSI 4016 CACGAG CACGAG(-5/-1)
BstEII 2465 GGTGACC G^GT[ACGT]ACC
BstPI 2465 GGTGACC G^GT[ACGT]ACC
BstXI 4138 CCAGACGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 126 CGGCCG C^GGCCG
EclXI 126 CGGCCG C^GGCCG
Eco52I 126 CGGCCG C^GGCCG
EcoO65I 2465 GGTGACC G^GT[ACGT]ACC
EcoRI 1217 GAATTC G^AATTC
FbaI 1927 TGATCA T^GATCA
FspI 5175 TGCGCA TGC^GCA
HindIII 367 AAGCTT A^AGCTT
KspI 123 CCGCGG CCGC^GG
NdeI 923 CATATG CA^TATG
PaeR7I 3821 CTCGAG C^TCGAG
PmaCI 3566 CACGTG CAC^GTG
PmlI 3566 CACGTG CAC^GTG
Psp1406I 5693 AACGTT AA^CGTT
RcaI 1624 TCATGA T^CATGA
SacI 5575 GAGCTC GAGCT^C
SacII 123 CCGCGG CCGC^GG
SfiI 103 GGCCCCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 4001 CACCGGCG C[GA]^CCGG[CT]G
SspBI 5712 TGTACA T^GTACA
SspI 395 AATATT AAT^ATT
XhoI 3821 CTCGAG C^TCGAG
XmnI 1027 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 3883 CTTAAG C^TTAAG
4337 CTTAAG
AflIII 1806 ACATGT A^C[GA][CT]GT
3758 ACATGT
AgeI 4074 ACCGGT A^CCGGT
4173 ACCGGT
Aor51HI 942 AGCGCT AGC^GCT
5561 AGCGCT
BalI 1149 TGGCCA TGG^CCA
4693 TGGCCA
BamHI 4935 GGATCC G^GATCC
5449 GGATCC
BfrI 3883 CTTAAG C^TTAAG
4337 CTTAAG
BsaAI 3566 CACGTG [CT]AC^GT[GA]
5351 CACGTA
BspLU11I 1806 ACATGT A^CATGT
3758 ACATGT
BsrBI 5472 CCGCTC CCGCTC(-3/-3)
4607 GAGCGG
Eco47III 942 AGCGCT AGC^GCT
5561 AGCGCT
EcoT22I 1157 ATGCAT ATGCA^T
1616 ATGCAT
HaeII 942 AGCGCT [GA]GCGC^[CT]
5561 AGCGCT
HgaI 5161 GACGC GACGC(5/10)
329 GCGTC
MluNI 1149 TGGCCA TGG^CCA
4693 TGGCCA
MscI 1149 TGGCCA TGG^CCA
4693 TGGCCA
NaeI 23 GCCGGC GCC^GGC
152 GCCGGC
NgoMIV 23 GCCGGC G^CCGGC
152 GCCGGC
NheI 3053 GCTAGC G^CTAGC
5564 GCTAGC
NsiI 1157 ATGCAT ATGCA^T
1616 ATGCAT
PinAI 4074 ACCGGT A^CCGGT
4173 ACCGGT
SapI 1544 GAAGAGC GCTCTTC(1/4)
3293 GAAGAGC
ScaI 5061 AGTACT AGT^ACT
5648 AGTACT
XcmI 2035 CCAGCACCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3943 CCATCTACAGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
Acc65I AccII AciI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI Asp718I AspHI AspI AvaI
AvaII BanI BanII BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SmaI SmlI SphI
Sse8387I StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XhoII XmaCI
XmaI