##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

Acc65I      AccIII      AflII       AgeI        AhdI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       AvrII       BbeI        BclI        
BfrI        BlnI        BseAI       BsiEI       BsiWI       
BsmI        BspEI       BspLU11I    BssHII      Bst1107I    
BstZ17I     CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      FbaI        FseI        
FspI        HpaI        KasI        KpnI        MfeI        
MluI        MroI        MunI        NarI        NotI        
NruI        PacI        PaeR7I      PflFI       PinAI       
PmeI        PshBI       PvuI        RsrII       SbfI        
ScaI        SfiI        SfoI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SwaI        Tth111I     
XhoI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII        1942  GACGTC               GACGT^C
AclI         4296  AACGTT               AA^CGTT
AflIII        778  ACGTGT               A^C[GA][CT]GT
Alw44I       1905  GTGCAC               G^TGCAC
Aor51HI      2842  AGCGCT               AGC^GCT
ApaI           42  GGGCCC               GGGCC^C
ApaLI        1905  GTGCAC               G^TGCAC
Asp700I      1175  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         2806  CCCGGG               C^[CT]CG[GA]G
BamHI          76  GGATCC               G^GATCC
BbrPI        2045  CACGTG               CAC^GTG
BbvCI        2553  GCTGAGG              CCTCAGC(-5/-2)
BcgI         3386  GCAGCTGCGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         433  AGATCT               A^GATCT
BsaBI        1884  GATCTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         3322  GAGACC               GGTCTC(1/5)
BsoBI        2806  CCCGGG               C^[CT]CG[GA]G
BspDI           4  ATCGAT               AT^CGAT
BsrBI         426  GAGCGG               CCGCTC(-3/-3)
BstBI         844  TTCGAA               TT^CGAA
ClaI            4  ATCGAT               AT^CGAT
DraI          407  TTTAAA               TTT^AAA
Eco47III     2842  AGCGCT               AGC^GCT
EcoRV        4682  GATATC               GAT^ATC
EcoT22I      2499  ATGCAT               ATGCA^T
MamI         1884  GATCTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         1810  GCCGGC               GCC^GGC
NdeI         1149  CATATG               CA^TATG
NgoMIV       1810  GCCGGC               G^CCGGC
NsiI         2499  ATGCAT               ATGCA^T
NspV          844  TTCGAA               TT^CGAA
PmaCI        2045  CACGTG               CAC^GTG
PmlI         2045  CACGTG               CAC^GTG
PshAI        3507  GACCTGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     4296  AACGTT               AA^CGTT
SalI          568  GTCGAC               G^TCGAC
SexAI        1506  ACCTGGT              A^CC[AT]GGT
SfuI          844  TTCGAA               TT^CGAA
SmaI         2806  CCCGGG               CCC^GGG
XbaI         3041  TCTAGA               T^CTAGA
XmaCI        2806  CCCGGG               C^CCGGG
XmaI         2806  CCCGGG               C^CCGGG
XmnI         1175  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

BalI         2419  TGGCCA               TGG^CCA
             2572  TGGCCA              
BciVI        5128  GGATAC               GTATCC(6/5)
             3379  GTATCC              
BsaAI        1416  TACGTG               [CT]AC^GT[GA]
             2045  CACGTG              
BsaWI         143  ACCGGA               [AT]^CCGG[AT]
             2149  TCCGGT              
BsmBI          19  GAGACG               CGTCTC(1/5)
             1875  GAGACG              
Bsp1407I     1145  TGTACA               T^GTACA
             4966  TGTACA              
BsrFI        1810  GCCGGC               [GA]^CCGG[CT]
             3247  GCCGGT              
BsrGI        1145  TGTACA               T^GTACA
             4966  TGTACA              
BstEII       3024  GGTGACC              G^GT[ACGT]ACC
             4121  GGTGACC             
BstPI        3024  GGTGACC              G^GT[ACGT]ACC
             4121  GGTGACC             
BstXI         667  CCAAAATGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3415  CCAGTCCACTGG        
Cfr10I       1810  GCCGGC               [GA]^CCGG[CT]
             3247  GCCGGT              
EcoO65I      3024  GGTGACC              G^GT[ACGT]ACC
             4121  GGTGACC             
FauI          188  CCCGC                CCCGC(4/6)
              255  CCCGC               
KspI           10  CCGCGG               CCGC^GG
              256  CCGCGG              
MluNI        2419  TGGCCA               TGG^CCA
             2572  TGGCCA              
MscI         2419  TGGCCA               TGG^CCA
             2572  TGGCCA              
NcoI         1643  CCATGG               C^CATGG
             3527  CCATGG              
NheI         3596  GCTAGC               G^CTAGC
             4771  GCTAGC              
NspI          729  ACATGC               [GA]CATG^[CT]
             1138  ACATGC              
PflMI        1321  CCAGTGGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3754  CCATTGGCTGG         
SacII          10  CCGCGG               CCGC^GG
              256  CCGCGG              
SspBI        1145  TGTACA               T^GTACA
             4966  TGTACA              
SspI         3927  AATATT               AAT^ATT
             4933  AATATT              
Van91I       1321  CCAGTGGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3754  CCATTGGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AluI        AlwI        AlwNI       AocI        
ApoI        AspHI       AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaHI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspHI       BspMI       BsrDI       BsrI        BssKI       
BssSI       BstAPI      BstF5I      BstNI       BstUI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRI       EcoRII      EcoT14I     Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       RcaI        RsaI        SacI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII