##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
Acc65I AflII AgeI Alw44I ApaLI
AscI AseI AsnI Asp718I AviII
BbrPI BclI BfrI BsaAI BsaBI
BsiWI BsmBI BssHII Bst1107I BstBI
BstZ17I EcoRI EcoRV FbaI FseI
FspI HpaI KpnI MamI MluI
NdeI NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI PvuI SbfI ScaI SfuI
SnaBI SpeI Sse8387I SspI StuI
SwaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AclI 1885 AACGTT AA^CGTT
AhdI 2170 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 59 AGCGCT AGC^GCT
Asp700I 2367 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2170 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4487 CCTAGG C^CTAGG
BglII 252 AGATCT A^GATCT
BlnI 4487 CCTAGG C^CTAGG
BlpI 906 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 906 GCTGAGC GC^T[ACGT]AGC
BsiEI 4 CGGCCG CG[GA][CT]^CG
BspDI 3391 ATCGAT AT^CGAT
BspLU11I 2252 ACATGT A^CATGT
BstXI 2543 CCACGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 906 GCTGAGC GC^T[ACGT]AGC
ClaI 3391 ATCGAT AT^CGAT
CpoI 2878 CGGTCCG CG^G[AT]CCG
EagI 4 CGGCCG C^GGCCG
Eam1105I 2170 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4 CGGCCG C^GGCCG
Eco47III 59 AGCGCT AGC^GCT
Eco52I 4 CGGCCG C^GGCCG
MfeI 422 CAATTG C^AATTG
MunI 422 CAATTG C^AATTG
PaeR7I 529 CTCGAG C^TCGAG
Psp1406I 1885 AACGTT AA^CGTT
RsrII 2878 CGGTCCG CG^G[AT]CCG
SalI 2137 GTCGAC G^TCGAC
SfiI 126 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2227 CACCGGCG C[GA]^CCGG[CT]G
XbaI 456 TCTAGA T^CTAGA
XhoI 529 CTCGAG C^TCGAG
XmnI 2367 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AatII 1894 GACGTC GACGT^C
3852 GACGTC
AccI 2137 GTCGAC GT^[AC][GT]AC
3574 GTCTAC
AccIII 3368 TCCGGA T^CCGGA
5436 TCCGGA
AcsI 381 AAATTT [GA]^AATT[CT]
1759 AAATTT
AocI 1047 CCTCAGG CC^T[ACGT]AGG
1366 CCTCAGG
ApoI 381 AAATTT [GA]^AATT[CT]
1759 AAATTT
BbvCI 4160 CCTCAGC CCTCAGC(-5/-2)
303 GCTGAGG
BcgI 2203 GCAAACCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2675 GCATGTTCTTCG
BseAI 3368 TCCGGA T^CCGGA
5436 TCCGGA
BspEI 3368 TCCGGA T^CCGGA
5436 TCCGGA
BstAPI 201 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2594 GCAACGTGTGC
Bsu36I 1047 CCTCAGG CC^T[ACGT]AGG
1366 CCTCAGG
DrdI 3565 GACTACAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4372 GACGGGCAGGTC
Eco81I 1047 CCTCAGG CC^T[ACGT]AGG
1366 CCTCAGG
EcoNI 2556 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3165 CCTGGGGGAGG
HindIII 3350 AAGCTT A^AGCTT
4397 AAGCTT
MroI 3368 TCCGGA T^CCGGA
5436 TCCGGA
NcoI 50 CCATGG C^CATGG
3485 CCATGG
SacI 3463 GAGCTC GAGCT^C
5463 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 155 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AspI AvaI AvaII BalI BamHI
BanI BanII BbeI BbsI BbvI
BciVI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
BssSI BstEII BstF5I BstNI BstPI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI EcoO109I
EcoO65I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NsiI NspI PflFI PflMI
PleI PpuMI PshAI PstI PvuII
RcaI RsaI SacII SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmaI SmlI SphI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI