##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AviII AvrII BbrPI BcgI
BciVI BlnI BsaAI BsaWI BseAI
BsiEI BsiWI BsmBI BspEI BssHII
BssSI BstBI BstEII BstPI DraI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRV EcoT22I FseI FspI HindIII
HpaI KpnI MluI MroI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
SalI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 3869 GTATAC GT^[AC][GT]AC
Alw44I 2201 GTGCAC G^TGCAC
ApaLI 2201 GTGCAC G^TGCAC
Asp700I 3255 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1494 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4648 TGGCCA TGG^CCA
BbeI 43 GGCGCC GGCGC^C
BbvCI 867 GCTGAGG CCTCAGC(-5/-2)
BclI 182 TGATCA T^GATCA
BsgI 3307 GTGCAG GTGCAG(16/14)
BsmI 4621 GAATGC GAATGC(1/-1)
BspDI 989 ATCGAT AT^CGAT
BspHI 2320 TCATGA T^CATGA
BsrBI 408 GAGCGG CCGCTC(-3/-3)
BsrDI 2361 CATTGC GCAATG(2/0)
Bst1107I 3869 GTATAC GTA^TAC
BstAPI 4469 GCACAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3869 GTATAC GTA^TAC
ClaI 989 ATCGAT AT^CGAT
CpoI 2593 CGGTCCG CG^G[AT]CCG
DrdI 4424 GACATGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1727 GAATTC G^AATTC
FbaI 182 TGATCA T^GATCA
HincII 3230 GTCAAC GT[CT]^[GA]AC
HindII 3230 GTCAAC GT[CT]^[GA]AC
KasI 43 GGCGCC G^GCGCC
KspI 92 CCGCGG CCGC^GG
MfeI 886 CAATTG C^AATTG
MluNI 4648 TGGCCA TGG^CCA
MscI 4648 TGGCCA TGG^CCA
MunI 886 CAATTG C^AATTG
NaeI 505 GCCGGC GCC^GGC
NarI 43 GGCGCC GG^CGCC
NgoMIV 505 GCCGGC G^CCGGC
PflFI 1494 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1546 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2320 TCATGA T^CATGA
RsrII 2593 CGGTCCG CG^G[AT]CCG
SacI 3833 GAGCTC GAGCT^C
SacII 92 CCGCGG CCGC^GG
SapI 1625 GAAGAGC GCTCTTC(1/4)
SbfI 4374 CCTGCAGG CCTGCA^GG
ScaI 2404 AGTACT AGT^ACT
SfoI 43 GGCGCC GGC^GCC
Sse8387I 4374 CCTGCAGG CCTGCA^GG
SspI 4108 AATATT AAT^ATT
Tth111I 1494 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1546 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 785 TCTAGA T^CTAGA
XmnI 3255 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 43 GGCGCC G[GA]^CG[CT]C
401 GGCGTC
AflII 807 CTTAAG C^TTAAG
2144 CTTAAG
AflIII 2416 ACATGT A^C[GA][CT]GT
3051 ACATGT
ApaI 540 GGGCCC GGGCC^C
665 GGGCCC
BfrI 807 CTTAAG C^TTAAG
2144 CTTAAG
BglII 1411 AGATCT A^GATCT
2306 AGATCT
BlpI 71 GCTAAGC GC^T[ACGT]AGC
3651 GCTCAGC
Bpu1102I 71 GCTAAGC GC^T[ACGT]AGC
3651 GCTCAGC
BsaBI 2301 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3332 GATGGAGATC
BsaHI 43 GGCGCC G[GA]^CG[CT]C
401 GGCGTC
BsaI 934 GAGACC GGTCTC(1/5)
3272 GGTCTC
Bsp1407I 1519 TGTACA T^GTACA
3579 TGTACA
BspLU11I 2416 ACATGT A^CATGT
3051 ACATGT
BsrFI 505 GCCGGC [GA]^CCGG[CT]
2370 ACCGGC
BsrGI 1519 TGTACA T^GTACA
3579 TGTACA
BstXI 1690 CCAGAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3291 CCATTGATATGG
CelII 71 GCTAAGC GC^T[ACGT]AGC
3651 GCTCAGC
Cfr10I 505 GCCGGC [GA]^CCGG[CT]
2370 ACCGGC
EaeI 507 CGGCCA [CT]^GGCC[GA]
4648 TGGCCA
Hin1I 43 GGCGCC G[GA]^CG[CT]C
401 GGCGTC
MamI 2301 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3332 GATGGAGATC
NcoI 397 CCATGG C^CATGG
1055 CCATGG
SspBI 1519 TGTACA T^GTACA
3579 TGTACA
StuI 1702 AGGCCT AGG^CCT
3686 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI Aor51HI ApoI
AspHI AvaI AvaII BamHI BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI