##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII Acc65I AclI AcsI ApoI
AscI Asp700I Asp718I AvrII BamHI
BciVI BglII BlnI BsaBI BsiWI
BspDI BspHI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DrdI EcoRI
EcoRV EcoT22I HpaI KpnI MamI
MluI NdeI NotI NruI NsiI
NspV PacI PmeI Psp1406I PvuI
RcaI RsrII ScaI SfuI SgrAI
SnaBI SpeI SspI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 3948 TCCGGA T^CCGGA
AflII 7001 CTTAAG C^TTAAG
AgeI 6424 ACCGGT A^CCGGT
AhdI 5120 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 5120 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 6122 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3495 TGCGCA TGC^GCA
BbeI 1941 GGCGCC GGCGC^C
BbrPI 6519 CACGTG CAC^GTG
BcgI 2808 CGAGGGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 7272 TGATCA T^GATCA
BfrI 7001 CTTAAG C^TTAAG
BseAI 3948 TCCGGA T^CCGGA
BspEI 3948 TCCGGA T^CCGGA
BspLU11I 1560 ACATGT A^CATGT
BstEII 3512 GGTTACC G^GT[ACGT]ACC
BstPI 3512 GGTTACC G^GT[ACGT]ACC
DraI 6827 TTTAAA TTT^AAA
EagI 2448 CGGCCG C^GGCCG
Eam1105I 5120 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2448 CGGCCG C^GGCCG
Eco52I 2448 CGGCCG C^GGCCG
EcoO65I 3512 GGTTACC G^GT[ACGT]ACC
FbaI 7272 TGATCA T^GATCA
FseI 2449 GGCCGGCC GGCCGG^CC
FspI 3495 TGCGCA TGC^GCA
HindIII 3893 AAGCTT A^AGCTT
KasI 1941 GGCGCC G^GCGCC
MfeI 7520 CAATTG C^AATTG
MroI 3948 TCCGGA T^CCGGA
MunI 7520 CAATTG C^AATTG
NaeI 2450 GCCGGC GCC^GGC
NarI 1941 GGCGCC GG^CGCC
NgoMIV 2450 GCCGGC G^CCGGC
PaeR7I 3989 CTCGAG C^TCGAG
PflFI 6122 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 6424 ACCGGT A^CCGGT
PmaCI 6519 CACGTG CAC^GTG
PmlI 6519 CACGTG CAC^GTG
PshAI 7316 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 5103 ACCAGGT A^CC[AT]GGT
SfiI 911 GGCCCCGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1941 GGCGCC GGC^GCC
SphI 3192 GCATGC GCATG^C
SwaI 6826 ATTTAAAT ATTT^AAAT
TfiI 344 GAATC G^A[AT]TC
Tth111I 6122 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3989 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 788 GTCGAC GT^[AC][GT]AC
2543 GTCGAC
AflIII 1485 ACACGT A^C[GA][CT]GT
1560 ACATGT
Alw44I 2421 GTGCAC G^TGCAC
3311 GTGCAC
ApaLI 2421 GTGCAC G^TGCAC
3311 GTGCAC
AseI 1016 ATTAAT AT^TAAT
1826 ATTAAT
AsnI 1016 ATTAAT AT^TAAT
1826 ATTAAT
BsaAI 1486 CACGTA [CT]AC^GT[GA]
6519 CACGTG
BsaI 3411 GAGACC GGTCTC(1/5)
6427 GGTCTC
BsiEI 1393 CGACCG CG[GA][CT]^CG
2448 CGGCCG
BsmI 3049 GAATGC GAATGC(1/-1)
5954 GCATTC
Bsp1407I 1785 TGTACA T^GTACA
3841 TGTACA
BsrGI 1785 TGTACA T^GTACA
3841 TGTACA
BssSI 2651 CACGAG CACGAG(-5/-1)
6771 CTCGTG
KspI 2587 CCGCGG CCGC^GG
3469 CCGCGG
NheI 1472 GCTAGC G^CTAGC
5714 GCTAGC
PshBI 1016 ATTAAT AT^TAAT
1826 ATTAAT
SacII 2587 CCGCGG CCGC^GG
3469 CCGCGG
SalI 788 GTCGAC G^TCGAC
2543 GTCGAC
SmaI 2551 CCCGGG CCC^GGG
5490 CCCGGG
SspBI 1785 TGTACA T^GTACA
3841 TGTACA
XmaCI 2551 CCCGGG C^CCGGG
5490 CCCGGG
XmaI 2551 CCCGGG C^CCGGG
5490 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmlI
Sse8387I StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII