##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AcyI AflII AgeI AhdI
Alw44I ApaLI AscI AspEI AspI
AviII BbeI BbrPI BclI BfrI
BlpI Bpu1102I BsaAI BsaHI BsiWI
BspDI BspHI BssHII BssSI BstBI
CelII ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV FbaI FseI FspI HgaI
Hin1I KasI MfeI MluI MunI
NaeI NarI NgoMIV NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PvuI RcaI RsrII
SalI SapI SbfI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 541 GGTACC G^GTACC
AccIII 429 TCCGGA T^CCGGA
AclI 4244 AACGTT AA^CGTT
AocI 2042 CCTCAGG CC^T[ACGT]AGG
Aor51HI 55 AGCGCT AGC^GCT
Asp700I 1685 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 541 GGTACC G^GTACC
AvaI 2344 CTCGAG C^[CT]CG[GA]G
AvrII 4727 CCTAGG C^CTAGG
BalI 2115 TGGCCA TGG^CCA
BamHI 1143 GGATCC G^GATCC
BciVI 2074 GTATCC GTATCC(6/5)
BlnI 4727 CCTAGG C^CTAGG
BsaBI 788 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 429 TCCGGA T^CCGGA
BsiEI 214 CGACCG CG[GA][CT]^CG
BsmBI 1356 GAGACG CGTCTC(1/5)
BsmI 1689 GCATTC GAATGC(1/-1)
BsoBI 2344 CTCGAG C^[CT]CG[GA]G
Bsp1407I 4349 TGTACA T^GTACA
BspEI 429 TCCGGA T^CCGGA
BsrBI 187 GAGCGG CCGCTC(-3/-3)
BsrFI 915 GCCGGT [GA]^CCGG[CT]
BsrGI 4349 TGTACA T^GTACA
Bst1107I 3636 GTATAC GTA^TAC
BstAPI 1233 GCAACTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 203 GGTTACC G^GT[ACGT]ACC
BstPI 203 GGTTACC G^GT[ACGT]ACC
BstXI 2295 CCAATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3636 GTATAC GTA^TAC
Bsu36I 2042 CCTCAGG CC^T[ACGT]AGG
Cfr10I 915 GCCGGT [GA]^CCGG[CT]
Eco47III 55 AGCGCT AGC^GCT
Eco81I 2042 CCTCAGG CC^T[ACGT]AGG
EcoNI 1345 CCTAGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 203 GGTTACC G^GT[ACGT]ACC
EcoT22I 1715 ATGCAT ATGCA^T
HaeII 55 AGCGCT [GA]GCGC^[CT]
HincII 3098 GTTAAC GT[CT]^[GA]AC
HindII 3098 GTTAAC GT[CT]^[GA]AC
HpaI 3098 GTTAAC GTT^AAC
KpnI 541 GGTACC GGTAC^C
KspI 243 CCGCGG CCGC^GG
MamI 788 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2115 TGGCCA TGG^CCA
MroI 429 TCCGGA T^CCGGA
MscI 2115 TGGCCA TGG^CCA
NheI 1570 GCTAGC G^CTAGC
NsiI 1715 ATGCAT ATGCA^T
PaeR7I 2344 CTCGAG C^TCGAG
PshAI 338 GACGAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4244 AACGTT AA^CGTT
PstI 2562 CTGCAG CTGCA^G
SacII 243 CCGCGG CCGC^GG
ScaI 3471 AGTACT AGT^ACT
SexAI 3569 ACCAGGT A^CC[AT]GGT
SfiI 63 GGCCTGACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 4349 TGTACA T^GTACA
SwaI 3967 ATTTAAAT ATTT^AAAT
XbaI 2705 TCTAGA T^CTAGA
XhoI 2344 CTCGAG C^TCGAG
XmnI 1685 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 2541 GTAGAC GT^[AC][GT]AC
3636 GTATAC
AflIII 3952 ACATGT A^C[GA][CT]GT
4352 ACATGT
ApaI 3007 GGGCCC GGGCC^C
4181 GGGCCC
BcgI 270 CGAGCCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1745 CGAAATCTGTGC
BglI 64 GCCTGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2301 GCCTGGAGGGC
BglII 1194 AGATCT A^GATCT
1812 AGATCT
BsgI 3294 CTGCAC GTGCAG(16/14)
1670 GTGCAG
BspLU11I 3952 ACATGT A^CATGT
4352 ACATGT
EaeI 1545 TGGCCG [CT]^GGCC[GA]
2115 TGGCCA
HindIII 1493 AAGCTT A^AGCTT
4125 AAGCTT
NcoI 2236 CCATGG C^CATGG
2719 CCATGG
NdeI 2850 CATATG CA^TATG
4646 CATATG
SacI 2571 GAGCTC GAGCT^C
3177 GAGCTC
TaqI 1699 TCGA T^CGA
2345 TCGA
TthHB8I 1699 TCGA T^CGA
2345 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PshBI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII